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公开(公告)号：US20210207122A1
公开(公告)日：2021-07-08
申请号：US15777577
申请日：2016-11-21
申请人： Robi David Mitra , Jeffrey Milbrandt , Ezra S. Abrams , Todd J. Barbera , T. Christian Boles , SAGE SCIENCE, INC. , WASHINGTON UNIVERSITY
发明人： Robi David MITRA , Jeffrey MILBRANDT , Ezra Solomon ABRAMS , Todd J. BARBERA , T. Christian BOLES
IPC分类号： C12N15/10 , G01N27/447
摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.
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公开(公告)号：US08361299B2
公开(公告)日：2013-01-29
申请号：US12760548
申请日：2010-04-14
IPC分类号： G01N27/453
CPC分类号： G01N27/44756 , G01N27/44791
摘要： The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.
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公开(公告)号：US08361298B2
公开(公告)日：2013-01-29
申请号：US12576148
申请日：2009-10-08
IPC分类号： G01N27/453
CPC分类号： G01N27/447 , G01N27/4473
摘要： The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.
摘要翻译： 本发明提供一种电泳盒，用于制作电泳盒的方法，以及在将样品单次施用到电泳系统中基于电泳迁移率从样品中分馏分析物的方法。
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公开(公告)号：US20110062024A1
公开(公告)日：2011-03-17
申请号：US12760548
申请日：2010-04-14
IPC分类号： G01N27/447 , G01N27/453 , G01F11/00
CPC分类号： G01N27/44756 , G01N27/44791
摘要： The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.
摘要翻译： 本发明提供一种电泳盒，用于制作电泳盒的方法，以及在将样品单次施用到电泳系统中基于电泳迁移率从样品中分馏分析物的方法。
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5.发明申请公开(公告)号：US20170240882A1
公开(公告)日：2017-08-24
申请号：US15519516
申请日：2015-10-15
申请人： Ezra S. ABRAMS , Danny YUN , Todd J. BARBERA , Douglas Grosvenor SABIN , T. Christian BOLES , SAGE SCIENCE, INC.
IPC分类号： C12N15/10 , G01N27/447 , C12Q1/68
CPC分类号： C12N15/101 , C12M47/06 , C12N15/1017 , C12Q1/6806 , G01N27/44747 , G01N27/44773 , G01N27/44782
摘要： Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.
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公开(公告)号：US20100126862A1
公开(公告)日：2010-05-27
申请号：US12576148
申请日：2009-10-08
IPC分类号： G01N33/559 , G01N27/26
CPC分类号： G01N27/447 , G01N27/4473
摘要： The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.
摘要翻译： 本发明提供一种电泳盒，用于制备电泳盒的方法，和从样品的单一应用基于电泳迁移率的电泳系统的样品分馏的分析物的方法。
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