公开(公告)号：US08981070B2

公开(公告)日：2015-03-17

申请号：US13866412

申请日：2013-04-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Simone Budow ,  Ping Ding ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Seela

IPC: C07H21/02 ,  C07H21/00 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C07H21/02

Abstract: An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is H or NH2, --- indicates a covalent bond, and said oligonucleotide is bound to said solid phase via the sulfur atom of said thiooxonucleotide. Also disclosed are methods for producing such conjugate as well as various uses for such oligonucleotide metal conjugate.

Abstract translation: 一种寡核苷酸 - 固相缀合物，其中所述固相是亲核的，并且所述寡核苷酸包含至少一种根据式I的硫氧基核碱基，其中X为CH或N，R 1为H或NH 2，表示共价键，所述寡核苷酸 通过所述硫代核苷酸的硫原子与所述固相结合。 还公开了用于生产这种缀合物的方法以及这种寡核苷酸金属缀合物的各种用途。