公开(公告)号：US20250115895A1

公开(公告)日：2025-04-10

申请号：US18676209

申请日：2024-05-28

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Stamatios Kokoris ,  Marc Prindle ,  Melud Nabavi ,  Craig Ostrander ,  Taylor Lehmann ,  Samantha Vellucci ,  Michael Kovarik ,  Jack Chase ,  Robert Busam ,  Miranda Lahman

IPC: C12N15/10 ,  C07H21/04 ,  C12N9/12

Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

公开(公告)号：US12037577B2

公开(公告)日：2024-07-16

申请号：US17653441

申请日：2022-03-03

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Mark Stamatios Kokoris ,  Marc Prindle ,  Melud Nabavi ,  Craig Ostrander ,  Taylor Lehmann ,  Samantha Vellucci ,  Michael Kovarik ,  Jack Chase ,  Robert Busam ,  Miranda Lahman

IPC: C12N9/12 ,  C07H21/04 ,  C12N15/09 ,  C12N15/10

CPC classification number: C12N15/10 ,  C07H21/04 ,  C12N9/1252 ,  C12Y207/07007

Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.