公开(公告)号：US12116570B2

公开(公告)日：2024-10-15

申请号：US16337282

申请日：2017-09-28

申请人： Roche Sequencing Solutions, Inc.

发明人： Mark Stamatios Kokoris ,  Michael Kovarik ,  Marc Prindle ,  Salka Keller ,  Robert Busam ,  Miranda Lahman

IPC分类号： C12N15/10 ,  C12N1/20 ,  C12N9/00 ,  C12N9/12 ,  C12N15/74 ,  C40B20/04 ,  C40B40/08

CPC分类号： C12N15/1058 ,  C12N1/20 ,  C12N9/1252 ,  C12N9/93 ,  C12N15/74 ,  C40B20/04 ,  C40B40/08 ,  C12Y207/07007

摘要： Methods and compositions for the selection of nucleic acid processing and other enzymes, and more specifically for the selection of DNA polymerases and other enzymes with desired properties employing the directed evolution of enzymes by plasmid tagging in droplets.

公开(公告)号：US20240240161A1

公开(公告)日：2024-07-18

申请号：US18328078

申请日：2023-06-02

申请人： Roche Sequencing Solutions, Inc.

发明人： Mark Stamatios Kokoris ,  Marc Prindle ,  Jack Chase ,  Robert Busam ,  Michael Kovarik ,  Salka Keller ,  Megan Murt ,  Greg Thiessen

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/6806

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6806 ,  C12Y207/07007

摘要： Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

公开(公告)号：US12037577B2

公开(公告)日：2024-07-16

申请号：US17653441

申请日：2022-03-03

申请人： Roche Sequencing Solutions, Inc.

发明人： Mark Stamatios Kokoris ,  Marc Prindle ,  Melud Nabavi ,  Craig Ostrander ,  Taylor Lehmann ,  Samantha Vellucci ,  Michael Kovarik ,  Jack Chase ,  Robert Busam ,  Miranda Lahman

IPC分类号： C12N9/12 ,  C07H21/04 ,  C12N15/09 ,  C12N15/10

CPC分类号： C12N15/10 ,  C07H21/04 ,  C12N9/1252 ,  C12Y207/07007

摘要： Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.