公开(公告)号：US20180252714A1

公开(公告)日：2018-09-06

申请号：US15974385

申请日：2018-05-08

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C07K1/107 ,  C07K7/06 ,  C12N9/10

CPC classification number: G01N33/573 ,  C07K1/1075 ,  C07K7/06 ,  C12N9/1044 ,  C12Y203/01013 ,  G01N2333/91085

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.

公开(公告)号：US10788495B2

公开(公告)日：2020-09-29

申请号：US15974385

申请日：2018-05-08

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C07K1/107 ,  C07K7/06 ,  C12N9/10

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.

公开(公告)号：US11280791B2

公开(公告)日：2022-03-22

申请号：US16991679

申请日：2020-08-12

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K1/107 ,  C07K7/06

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

公开(公告)号：US20200249231A1

公开(公告)日：2020-08-06

申请号：US16785406

申请日：2020-02-07

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K7/06 ,  C07K1/107

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

公开(公告)号：US10006915B2

公开(公告)日：2018-06-26

申请号：US14973472

申请日：2015-12-17

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: C07K7/06 ,  G01N33/573 ,  C12N9/10 ,  C07K1/107

CPC classification number: G01N33/573 ,  C07K1/1075 ,  C07K7/06 ,  C12N9/1044 ,  C12Y203/01013 ,  G01N2333/91085

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.

公开(公告)号：US11268120B2

公开(公告)日：2022-03-08

申请号：US15625784

申请日：2017-06-16

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen

IPC: C07K16/28 ,  C07K16/30 ,  A61K38/08 ,  C12P21/02 ,  C12N9/10 ,  C07K14/47 ,  C07K1/107 ,  C07K1/13 ,  C07K14/00

Abstract: According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.

公开(公告)号：US11054425B2

公开(公告)日：2021-07-06

申请号：US16785406

申请日：2020-02-07

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K1/107 ,  C07K7/06

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

公开(公告)号：US20200371102A1

公开(公告)日：2020-11-26

申请号：US16991679

申请日：2020-08-12

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K1/107 ,  C07K7/06

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.