公开(公告)号：US10788495B2

公开(公告)日：2020-09-29

申请号：US15974385

申请日：2018-05-08

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C07K1/107 ,  C07K7/06 ,  C12N9/10

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.

公开(公告)号：US10427126B2

公开(公告)日：2019-10-01

申请号：US15259893

申请日：2016-09-08

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Ryan Bannen ,  Sarah Barilovits ,  Jigar Patel ,  Eric Sullivan ,  John Tan

IPC: B01J19/00 ,  G01N33/68 ,  C07K1/04 ,  C07K17/14 ,  G01N21/64 ,  C40B50/16

Abstract: The present invention provides a system and method for assessing a synthetic peptide population including interrogating a population of peptide features in the presence of a receptor having an affinity for a binder sequence. The population of peptide features is synthesized over a plurality of synthesis periods and includes a plurality of control peptide features synthesized to have an amino acid sequence including the binder sequence. The control peptide features include a first feature synthesized beginning with a first one of the synthesis periods, and a second feature synthesized beginning after the first one of the synthesis periods such that synthesis of the second control peptide feature is delayed by at least one synthesis period. The method further includes detecting a signal output characteristic of an interaction of the receptor with the control peptide features, the signal output indicative of the fidelity of synthesis of the population of peptide features.

公开(公告)号：US10184939B2

公开(公告)日：2019-01-22

申请号：US15423768

申请日：2017-02-03

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Ryan Bannen ,  Jigar Patel ,  Ken Lo

IPC: G01N33/564 ,  G01N33/543 ,  G01N33/574 ,  G01N33/68

Abstract: The present disclosure provides a method of identifying a peptide antigen, including contacting an antibody-comprising composition to a peptide microarray having an array surface and a plurality of feature pairs disposed thereon. Each of the feature pairs includes a first feature of wild-type peptides having a defined sequence and a second feature of mutant peptides having the defined sequence with a single amino acid mutation. Each of the wild-type peptides and the mutant peptides has an array-coupled terminus coupled to the array surface and an opposing free terminus, and the position of the amino acid mutation in the mutant peptides is located closer to the array-coupled terminus of the mutant peptides than the free terminus. The method further includes detecting binding of an antibody in the antibody-comprising composition to at least one of the feature pairs on the peptide microarray, and identifying feature pairs exhibiting differential binding.

公开(公告)号：US11280791B2

公开(公告)日：2022-03-22

申请号：US16991679

申请日：2020-08-12

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K1/107 ,  C07K7/06

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

公开(公告)号：US20200249231A1

公开(公告)日：2020-08-06

申请号：US16785406

申请日：2020-02-07

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K7/06 ,  C07K1/107

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

公开(公告)号：US10006915B2

公开(公告)日：2018-06-26

申请号：US14973472

申请日：2015-12-17

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: C07K7/06 ,  G01N33/573 ,  C12N9/10 ,  C07K1/107

CPC classification number: G01N33/573 ,  C07K1/1075 ,  C07K7/06 ,  C12N9/1044 ,  C12Y203/01013 ,  G01N2333/91085

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.

公开(公告)号：US20170059578A1

公开(公告)日：2017-03-02

申请号：US15233543

申请日：2016-08-10

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Ryan Bannen ,  Sarah Barilovits ,  Jigar Patel ,  Eric Sullivan ,  John Tan

IPC: G01N33/68 ,  G01N21/64 ,  B01J19/00

CPC classification number: G01N33/6845 ,  B01J19/0046 ,  B01J2219/00623 ,  B01J2219/00659 ,  B01J2219/00693 ,  B01J2219/00711 ,  B01J2219/00725 ,  G01N21/6428 ,  G01N21/6452

Abstract: The present invention provides a system and method for assessing the fidelity of a synthetic peptide population including interrogating a population of peptide features in the presence of a receptor having an affinity for a plurality of binder sequences. A first amino acid is at a defined position within a first one of the binder sequences, and the population of peptide features includes a first control peptide feature synthesized to have an amino acid sequence including the first one of the binder sequences. The system and method further includes detecting a signal output characteristic of an interaction of the receptor with the first control peptide feature. The signal output is indicative of the fidelity of incorporation of the first amino acid into the first control peptide at the defined position within the first one of the binder sequences.

Abstract translation: 本发明提供了用于评估合成肽群体的保真度的系统和方法，包括在存在对多个粘合剂序列具有亲和力的受体的情况下询问肽特征群体。 第一个氨基酸位于第一个粘合剂序列内的一个定义的位置，并且肽特征的群体包括合成为具有包括第一个粘合剂序列的氨基酸序列的第一对照肽特征。 该系统和方法还包括检测受体与第一对照肽特征的相互作用的信号输出特性。 信号输出指示在第一个粘合剂序列中的限定位置将第一个氨基酸并入第一个对照肽的保真度。

公开(公告)号：US11268120B2

公开(公告)日：2022-03-08

申请号：US15625784

申请日：2017-06-16

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen

IPC: C07K16/28 ,  C07K16/30 ,  A61K38/08 ,  C12P21/02 ,  C12N9/10 ,  C07K14/47 ,  C07K1/107 ,  C07K1/13 ,  C07K14/00

Abstract: According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.

公开(公告)号：US11054425B2

公开(公告)日：2021-07-06

申请号：US16785406

申请日：2020-02-07

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Thomas Albert ,  Frank Bergmann ,  Victor Lyamichev ,  Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel ,  Jigar Patel ,  Michael Schraeml ,  Wojtek Steffen ,  Thomas Streidl

IPC: G01N33/573 ,  C12N9/10 ,  C07K1/107 ,  C07K7/06

Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

公开(公告)号：US10976317B2

公开(公告)日：2021-04-13

申请号：US16191407

申请日：2018-11-14

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Jigar Patel ,  Victor Lyamichev ,  Thomas Albert

IPC: C40B30/04 ,  G01N33/573 ,  C07K1/04 ,  G01N33/574 ,  G01N33/68 ,  C07K7/06

Abstract: The invention comprises systems, methods and arrays for identification and optimization of novel peptide binders to protein targets. Embodiments include steps of peptide binder discovery, core peptide maturation, N-terminal and C-terminal extension and kinetics analysis of the final peptide binder.