公开(公告)号：US20210115510A1

公开(公告)日：2021-04-22

申请号：US16945099

申请日：2020-07-31

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Daleen Badenhorst ,  Michael S. Berry ,  Richard Dannebaum ,  Ashley Hayes ,  Severine Margeridon ,  Martin Ranik ,  Etienne Slabbert

IPC分类号： C12Q1/6874

摘要： The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of single stranded circular target nucleic acids.

公开(公告)号：US20200308576A1

公开(公告)日：2020-10-01

申请号：US16808173

申请日：2020-03-03

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Daleen Badenhorst ,  Richard Dannebaum ,  Ashley Hayes ,  Monica Herrera ,  Severine Margeridon ,  Martin Ranik

IPC分类号： C12N15/10 ,  C12Q1/6874

摘要： The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation.

公开(公告)号：US11345955B2

公开(公告)日：2022-05-31

申请号：US16813379

申请日：2020-03-09

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Daleen Badenhorst ,  Richard Dannebaum ,  Ashley Hayes ,  Monica Herrera ,  Severine Margeridon ,  Martin Ranik

IPC分类号： C12P19/34 ,  C12Q1/6851 ,  C12N15/10 ,  C12Q1/6869

摘要： The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation. In particular, disclosed are methods of making circular target nucleic acid molecules and libraries of such molecules for downstream analysis such as nucleic acid sequencing. The method comprises the steps of adding universal sequences to nucleic acid molecules, rendering single-stranded these nucleic acid molecules with universal sequences on their ends by contacting with a probe complementary to at least a portion of the universal sequences, and allowing the hybridized probe to enable circularization and formation of single-stranded circular (sscDNA) molecules.

公开(公告)号：US12110534B2

公开(公告)日：2024-10-08

申请号：US16945099

申请日：2020-07-31

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Daleen Badenhorst ,  Michael S. Berry ,  Richard Dannebaum ,  Ashley Hayes ,  Severine Margeridon ,  Martin Ranik ,  Etienne Slabbert

IPC分类号： C12Q1/6806 ,  C12Q1/6869

CPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/319 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2525/307 ,  C12Q2535/122 ,  C12Q1/6869 ,  C12Q2525/307 ,  C12Q2535/122 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2525/155 ,  C12Q2521/319

摘要： The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of single stranded circular target nucleic acids.

公开(公告)号：US11898204B2

公开(公告)日：2024-02-13

申请号：US16977421

申请日：2019-02-28

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Aruna Arcot ,  Daleen Badenhorst ,  Rui Chen ,  Toummy Guettouche ,  Ashley Hayes ,  Jenny A. Johnson ,  Severine Margeridon ,  Martin Ranik ,  Persis Wadia ,  Alexandra Hui Wang

IPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6855

CPC分类号： C12Q1/6874 ,  C12N15/1068 ,  C12Q1/6855

摘要： The invention is a novel method of separately sequencing each strand of a nucleic acid involving the use of an adaptor comprising a strand cleavage site or a strand synthesis termination site. The adaptor may also be self-priming at the strand cleavage site.

公开(公告)号：US20230183797A1

公开(公告)日：2023-06-15

申请号：US16977421

申请日：2019-02-28

申请人： Roche Sequencing Solutions, Inc. ,  Kapa Biosystems, Inc.

发明人： Aruna Arcot ,  Daleen Badenhorst ,  Rui Chen ,  Toummy Guettouche ,  Ashley Hayes ,  Jenny A. Johnson ,  Severine Margeridon ,  Martin Ranik ,  Persis Wadia ,  Alexandra Hui Wang

IPC分类号： C12Q1/6874 ,  C12Q1/6855 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12Q1/6855 ,  C12N15/1068

摘要： The invention is a novel method of separately sequencing each strand of a nucleic acid involving the use of an adaptor comprising a strand cleavage site or a strand synthesis termination site. The adaptor may also be self-priming at the strand cleavage site.