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    PB 39, a gene dysregulated in prostate cancer, and uses thereof
    1.
    发明授权
    PB 39, a gene dysregulated in prostate cancer, and uses thereof 失效
    PB39，前列腺癌失调的基因及其用途

    公开(公告)号：US07226731B1

    公开(公告)日：2007-06-05

    申请号：US09743825

    申请日：1999-07-23

    申请人： Rodrigo F. Chuaqui ,  Kristina A. Cole ,  Lance A. Liotta ,  Michael R. Emmert-Buck

    发明人： Rodrigo F. Chuaqui ,  Kristina A. Cole ,  Lance A. Liotta ,  Michael R. Emmert-Buck

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/158

    摘要： A novel gene, PB39, that is up-regulated, or over-expressed, in prostate cancer has been identified. The gene has been identified by means of its cDNA obtained by reverse transcription of the corresponding mRNA. Microdissection of prostate glands that had been surgically removed from prostate cancer patients revealed a novel up-regulated transcript in an aggressive prostate carcinoma. Differential analysis for the presence of this gene was carried out from the same glands by comparing transcription in microdissected normal prostatic epithelium versus that in microdissected invasive tumor. The transcript was over-expressed in 5 of 10 prostate carcinomas examined. A variant transcript was over-expressed in 4 of 4 prostate carcinomas, and was found in 1 of 4 normal samples. The invention provides a purified and isolated nucleic acid that includes the sequence of PB39 or its complement, the sequence of a variant of PB39 or its complement, and a primer or probe, that includes a sequence that is a fragment of these sequences. Additionally, the polypeptide encoded by these genes, an antibody to the polypeptide, and methods of detection of PB39 or its gene product are provided.

    摘要翻译： 已经鉴定出在前列腺癌中上调或过度表达的新基因PB39。 该基因已通过其相应mRNA的逆转录获得的cDNA鉴定。 从前列腺癌患者手术切除的前列腺的显微解剖显示在侵袭性前列腺癌中有一种新的上调的转录物。 通过比较显微解剖正常前列腺上皮中的转录与微切割侵袭性肿瘤中的转录，从相同腺体进行差异分析。 在检查的10个前列腺癌中的5个中，转录物过表达。 在4个前列腺癌中的4个中，变体转录物过表达，并且在4个正常样品中的1个中发现。 本发明提供了纯化和分离的核酸，其包括PB39或其互补序列，PB39或其互补序列变体的序列，以及引物或探针，其包括作为这些序列的片段的序列。 另外，提供了由这些基因编码的多肽，多肽的抗体，以及检测PB39或其基因产物的方法。

    Target activated microtransfer
    3.
    发明授权
    Target activated microtransfer 有权
    靶活化微转移

    公开(公告)号：US08460744B2

    公开(公告)日：2013-06-11

    申请号：US12713105

    申请日：2010-02-25

    申请人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

    发明人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

    IPC分类号： B05D1/00

    CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

    摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

    摘要翻译： 一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。 还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。 该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及用于在照射期间向转印膜施加恒定压力的装置。

    TARGET ACTIVATED MICROTRANSFER
    4.
    发明申请
    TARGET ACTIVATED MICROTRANSFER 有权
    目标活化微量RNA

    公开(公告)号：US20100216166A1

    公开(公告)日：2010-08-26

    申请号：US12713105

    申请日：2010-02-25

    申请人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

    发明人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

    IPC分类号： G01N33/567

    CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

    摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

    摘要翻译： 一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。 还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。 该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及在照射期间向转印膜施加恒定压力的装置。

    Target activated microtransfer
    6.
    发明授权
    Target activated microtransfer 有权
    靶活化微转移

    公开(公告)号：US07695752B2

    公开(公告)日：2010-04-13

    申请号：US11202848

    申请日：2005-08-12

    申请人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

    发明人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

    IPC分类号： B05D1/00

    CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

    摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

    摘要翻译： 一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。 还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。 该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑性层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及用于在照射期间向转印膜施加恒定压力的装置。