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公开(公告)号:US5453372A
公开(公告)日:1995-09-26
申请号:US918318
申请日:1992-07-27
申请人: Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
发明人: Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
CPC分类号: C11D3/38663 , C11D3/386 , C12N9/00 , C12N9/20 , C12N9/54
摘要: Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.
摘要翻译: 针对离子表面活性剂的不稳定作用而进行的酶稳定化,以及生产这样的稳定化酶的方法,其中编码酶的DNA序列通过与定义的酶的表面区域相关的限定位置的定向突变来修饰, 突变位于的密码子现在编码与原始氨基酸不同的氨基酸。