Highly alkaline proteases
    2.
    发明授权
    Highly alkaline proteases 失效
    高碱性蛋白酶

    公开(公告)号:US5352603A

    公开(公告)日:1994-10-04

    申请号:US661378

    申请日:1991-02-28

    CPC分类号: C12N9/54

    摘要: Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

    摘要翻译: 通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。 突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得,通过定点诱变(点突变)以定点诱变(点突变)改变这些DNA序列,使得点突变位于的密码子现在 编码比原始氨基酸更强碱性的氨基酸。 结果是高碱性蛋白酶,其中原始氨基酸已被更强碱性氨基酸替代,优选由氨基酸赖氨酸或精氨酸代替。 还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸,DNA序列,载体和转化微生物。

    Process for the preparation of festuclavine
    6.
    发明授权
    Process for the preparation of festuclavine 失效
    制备festuklavine的方法

    公开(公告)号:US4939089A

    公开(公告)日:1990-07-03

    申请号:US842110

    申请日:1986-02-03

    IPC分类号: C12P17/18 C12R1/645

    CPC分类号: C12P17/183 Y10S435/911

    摘要: A process is claimed for the preparation of festuclavine, characterized by cultivating the microorganism Claviceps paspali 2838 and isolating the thus-formed festuclavine after termination of fermentation.

    摘要翻译: PCT No.PCT / DE85 / 00189 Sec。 371日期1986年2月3日 102(e)日期1986年2月3日PCT提交1985年5月30日PCT公布。 第WO85 / 05634号公报 1985年12月19日授权。要求用于制备festuclavine的方法,其特征在于培养微生物Claviceps paspali 2838并在发酵终止后分离由此形成的festuclavine。

    Process for the extraction of amino acids from an aqueous phase
    7.
    发明授权
    Process for the extraction of amino acids from an aqueous phase 失效
    从水相中提取氨基酸的方法

    公开(公告)号:US4644067A

    公开(公告)日:1987-02-17

    申请号:US690546

    申请日:1985-01-11

    CPC分类号: C07D209/20

    摘要: A process for the extraction of aromatic (e.g., phenyl-containing) amino acids from an aqueous phase (solution) containing them, comprising combining the aqueous phase with a tenside of the formulaR--U--SO.sub.3 XwhereinR is alkyl or cycloalkyl each of 4-18 carbon atoms; or phenyl or naphthyl, each optionally substituted by alkyl groups, and having up to 18 carbon atoms total.U is a carbon-to-sulfur bond or an oxygen atom, andX is an alkali metal ion;acidifying the aqueous phase to a pH value of 2.0-2.5; andextracting the aqueous phase with an alcohol, a ketone, or a carboxylic acid alkyl ester, each containing 4-6 carbon atoms.

    摘要翻译: 一种从含有它们的水相(溶液)中提取芳族(例如含苯基)氨基酸的方法,包括将水相与式R U-SO 3 X的表面活性剂结合,其中R是烷基或环烷基, 18个碳原子; 或苯基或萘基,各自任选被烷基取代,并且总共具有至多18个碳原子。 U是碳 - 硫键或氧原子,X是碱金属离子; 将水相酸化至pH值为2.0-2.5; 并用含有4-6个碳原子的醇,酮或羧酸烷基酯萃取水相。

    Process for the preparation of chanoclavine
    9.
    发明授权
    Process for the preparation of chanoclavine 失效
    氯喹啉的制备方法

    公开(公告)号:US4968610A

    公开(公告)日:1990-11-06

    申请号:US842704

    申请日:1986-02-03

    CPC分类号: C12P17/10 Y10S435/911

    摘要: A process is claimed for the preparation of chanoclavine, characterized by cultivating the microorganism Claviceps spec. DSM 2837 and isolating the thus-formed chanoclavine after termination of fermentation.

    摘要翻译: PCT No.PCT / DE85 / 00191 Sec。 371日期1986年2月3日 102(e)日期1986年2月3日PCT提交1985年5月30日PCT公布。 公开号WO85 / 05633 1985年12月19日的日期。声称用于制备chanoclavine的方法,其特征在于培养微生物Claviceps规格。 DSM 2837,并在发酵终止后分离如此形成的chanoclavine。