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公开(公告)号：US5453372A
公开(公告)日：1995-09-26
申请号：US918318
申请日：1992-07-27
申请人： Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
发明人： Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
CPC分类号： C11D3/38663 , C11D3/386 , C12N9/00 , C12N9/20 , C12N9/54
摘要： Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.
摘要翻译： 针对离子表面活性剂的不稳定作用而进行的酶稳定化，以及生产这样的稳定化酶的方法，其中编码酶的DNA序列通过与定义的酶的表面区域相关的限定位置的定向突变来修饰， 突变位于的密码子现在编码与原始氨基酸不同的氨基酸。
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公开(公告)号：US5352603A
公开(公告)日：1994-10-04
申请号：US661378
申请日：1991-02-28
申请人： Roman Vetter , Detlef Wilke , Antoine Amory , Andre Clippe , Dietmar Schomburg , Wolfgang Aehle
发明人： Roman Vetter , Detlef Wilke , Antoine Amory , Andre Clippe , Dietmar Schomburg , Wolfgang Aehle
CPC分类号： C12N9/54
摘要： Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.
摘要翻译： 通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。 突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得，通过定点诱变（点突变）以定点诱变（点突变）改变这些DNA序列，使得点突变位于的密码子现在 编码比原始氨基酸更强碱性的氨基酸。 结果是高碱性蛋白酶，其中原始氨基酸已被更强碱性氨基酸替代，优选由氨基酸赖氨酸或精氨酸代替。 还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸，DNA序列，载体和转化微生物。
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公开(公告)号：US5880080A
公开(公告)日：1999-03-09
申请号：US413724
申请日：1995-03-31
IPC分类号： C12N15/09 , C11D3/386 , C11D3/39 , C11D7/42 , C12N1/21 , C12N9/54 , C12N15/00 , C12R1/07 , C12R1/19 , D06L1/12 , B08B1/00
摘要： The use of alkaline bacillus proteases in commercial laundry methods and compositions containing these proteases for commercial laundering are described.
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公开(公告)号：US6165960A
公开(公告)日：2000-12-26
申请号：US853494
申请日：1997-05-08
IPC分类号： C12N15/09 , C11D3/386 , C11D3/39 , C11D7/42 , C12N1/21 , C12N9/54 , C12N15/00 , C12R1/07 , C12R1/19 , D06L1/12 , C11D3/395
摘要： The use of alkaline bacillus proteases in commercial laundry methods and compositions containing these proteases for commercial laundering are described.
摘要翻译： 描述了在商业洗衣方法中使用碱性芽孢杆菌蛋白酶和含有这些蛋白酶用于商业洗涤的组合物。
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