公开(公告)号：US5389537A

公开(公告)日：1995-02-14

申请号：US184604

申请日：1994-01-21

申请人： Ronald T. Raines ,  Stephen B. del Cardayre

发明人： Ronald T. Raines ,  Stephen B. del Cardayre

IPC分类号： C12N9/22

CPC分类号： C12N9/22 ,  Y10S435/81

摘要： A ribonuclease molecule altered at a single amino acid, relative to its wild-type form, displays altered substrate specificity and substrate binding mechanism. The altered protein cleaves RNA efficiently after C, U and A residues, whereas the wild-type protein cannot cleave efficiently after A. The change that alters the specificity also permits the protein to cleave poly (A) portions of an RNA molecule processively.

摘要翻译： 相对于其野生型形式，在单个氨基酸处改变的核糖核酸酶分子显示改变的底物特异性和底物结合机制。 改变的蛋白质在C，U和A残基之后有效地切割RNA，而野生型蛋白质在A后不能有效切割。改变特异性的变化也允许蛋白质切割RNA分子的聚（A）部分。

公开(公告)号：US20090084025A1

公开(公告)日：2009-04-02

申请号：US12239562

申请日：2008-09-26

申请人： Monica Bhatia ,  Michael C.M. Cockrem ,  Stephen B. del Cardayre ,  Fernando A. Sanchez-Riera

发明人： Monica Bhatia ,  Michael C.M. Cockrem ,  Stephen B. del Cardayre ,  Fernando A. Sanchez-Riera

IPC分类号： C10L1/18 ,  C12P5/00 ,  C12P7/64 ,  C12P7/04

CPC分类号： C12P7/649 ,  Y02E50/13

摘要： There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.

摘要翻译： 提供了改善来自工程微生物细胞的生物产物生产的生物产物和方法，所述方法包括：提供包含粗碳源的发酵液; 用所述微生物细胞接种所述发酵液; 并培养接种的发酵液; 其中所述生物产物是与所述发酵液不混溶的疏水性溶剂，并且其中存在于所述粗碳源中的有毒副产物可溶于所述疏水溶剂。 此外，提供了用于实践改善生物产物生产方法的试剂盒。

公开(公告)号：US20100242345A1

公开(公告)日：2010-09-30

申请号：US12278957

申请日：2007-05-18

申请人： Jay D. Keasling ,  Zhihao Hu ,  Chris Sommerville ,  George Church ,  David Berry ,  Lisa Friedman ,  Andreas Schirmer ,  Shane Brubaker ,  Stephen B. del Cardayre

发明人： Jay D. Keasling ,  Zhihao Hu ,  Chris Sommerville ,  George Church ,  David Berry ,  Lisa Friedman ,  Andreas Schirmer ,  Shane Brubaker ,  Stephen B. del Cardayre

IPC分类号： C12N1/00 ,  C12N1/36 ,  C07C57/03 ,  C12N1/21 ,  C12P7/64 ,  C10L1/19 ,  C12N1/19

CPC分类号： C12P7/04 ,  C07C31/125 ,  C07C33/02 ,  C10L1/026 ,  C10L1/328 ,  C12P7/6436 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

摘要： Genetically engineered microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use.

摘要翻译： 提供从脂肪酸生物合成途径（脂肪酸衍生物）产生产物的基因工程微生物以及其使用方法。

公开(公告)号：US08313934B2

公开(公告)日：2012-11-20

申请号：US12239562

申请日：2008-09-26

申请人： Monica Bhatia ,  Michael C. M. Cockrem ,  Stephen B. del Cardayre ,  Fernando A. Sanchez-Riera

发明人： Monica Bhatia ,  Michael C. M. Cockrem ,  Stephen B. del Cardayre ,  Fernando A. Sanchez-Riera

IPC分类号： C12P7/62

CPC分类号： C12P7/649 ,  Y02E50/13

摘要： There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.

摘要翻译： 提供了改善来自工程微生物细胞的生物产物生产的生物产物和方法，所述方法包括：提供包含粗碳源的发酵液; 用所述微生物细胞接种所述发酵液; 并培养接种的发酵液; 其中所述生物产物是与所述发酵液不混溶的疏水性溶剂，并且其中存在于所述粗碳源中的有毒副产物可溶于所述疏水溶剂。 此外，提供了用于实践改善生物产物生产方法的试剂盒。