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公开(公告)号：US08535910B2
公开(公告)日：2013-09-17
申请号：US13349514
申请日：2012-01-12
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
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公开(公告)号：US20090298125A1
公开(公告)日：2009-12-03
申请号：US12505374
申请日：2009-07-17
申请人： S. Christopher Davis , Richard J. Fox , Gjalt W. Huisman , Vesna Gavrilovic , Emily C. Mundorff , Lisa M. Newman
发明人： S. Christopher Davis , Richard J. Fox , Gjalt W. Huisman , Vesna Gavrilovic , Emily C. Mundorff , Lisa M. Newman
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
摘要翻译： 本发明涉及新型卤代醇脱卤素酶多肽和编码它们的多核苷酸。 这些多肽可用于制备4-取代-3-丁酸衍生物和连位氰基羟基取代的羧酸酯。 本发明还提供相关载体，宿主细胞和方法。
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3.发明授权Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters 有权用于生产4-取代的3-羟基丁酸衍生物和连位氰基羟基取代的羧酸酯的酶法公开(公告)号：US07132267B2
公开(公告)日：2006-11-07
申请号：US10782258
申请日：2004-02-18
申请人： S. Christopher Davis , John H. Grate , David R. Gray , John M. Gruber , Gjalt W. Huisman , Steven K. Ma , Lisa M. Newman , Roger Sheldon , Li A Wang
发明人： S. Christopher Davis , John H. Grate , David R. Gray , John M. Gruber , Gjalt W. Huisman , Steven K. Ma , Lisa M. Newman , Roger Sheldon , Li A Wang
IPC分类号： C12P13/00
CPC分类号： C12P13/002 , C12N9/14 , C12P7/62 , C12P13/02
摘要： The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives The present invention also provides methods and compositions for preparing vicinal cyano, hydroxyl substituted carboxylic acid esters.
摘要翻译： 本发明提供了通过卤代醇脱卤酶催化的4-卤代-3-羟基丁酸衍生物的转化来制备4-取代的3-羟基丁酸衍生物的方法和组合物。 本发明还提供了通过酮还原酶催化转化4-卤代-3-酮丁酸衍生物来制备4-卤代-3-羟基丁酸衍生物的方法和组合物。本发明还提供了用于制备邻位氰基，羟基取代的羧酸的方法和组合物 酸酯。
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4.发明授权Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives 有权用于生产4-取代的3-羟基丁酸衍生物的酶法公开(公告)号：US07807423B2
公开(公告)日：2010-10-05
申请号：US11502745
申请日：2006-08-10
申请人： S. Christopher Davis , John H. Grate , David R. Gray , John M. Gruber , Gjalt W. Huisman , Steven K. Ma , Lisa M. Newman , Roger Sheldon , Li A Wang
发明人： S. Christopher Davis , John H. Grate , David R. Gray , John M. Gruber , Gjalt W. Huisman , Steven K. Ma , Lisa M. Newman , Roger Sheldon , Li A Wang
CPC分类号： C12P13/002 , C12N9/14 , C12P7/62 , C12P13/02
摘要： The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives The present invention also provides methods and compositions for preparing vicinal cyano, hydroxyl substituted carboxylic acid esters.
摘要翻译： 本发明提供了通过卤代醇脱卤酶催化的4-卤代-3-羟基丁酸衍生物的转化来制备4-取代的3-羟基丁酸衍生物的方法和组合物。 本发明还提供了通过酮还原酶催化转化4-卤代-3-酮丁酸衍生物来制备4-卤代-3-羟基丁酸衍生物的方法和组合物。本发明还提供了用于制备邻位氰基，羟基取代的羧酸的方法和组合物 酸酯。
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5.发明授权Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives 有权用于生产4-取代的3-羟基丁酸衍生物的酶法公开(公告)号：US07125693B2
公开(公告)日：2006-10-24
申请号：US10639159
申请日：2003-08-11
申请人： S. Christopher Davis , John H. Grate , David R. Gray , John M. Gruber , Gjalt W. Huisman , Steven K. Ma , Lisa M. Newman , Roger Sheldon , Li A Wang
发明人： S. Christopher Davis , John H. Grate , David R. Gray , John M. Gruber , Gjalt W. Huisman , Steven K. Ma , Lisa M. Newman , Roger Sheldon , Li A Wang
IPC分类号： C12P13/00
CPC分类号： C12P13/002 , C12N9/14 , C12P13/02
摘要： The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives.
摘要翻译： 本发明提供了通过卤代醇脱卤酶催化的4-卤代-3-羟基丁酸衍生物的转化来制备4-取代的3-羟基丁酸衍生物的方法和组合物。 本发明还提供了通过酮还原酶催化转化4-卤代-3-酮丁酸衍生物制备4-卤代-3-羟基丁酸衍生物的方法和组合物。
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公开(公告)号：US08101395B2
公开(公告)日：2012-01-24
申请号：US12573824
申请日：2009-10-05
申请人： S. Christopher Davis , Richard J. Fox , Gjalt W. Huisman , Vesna Gavrilovic , Lisa Marie Newman
发明人： S. Christopher Davis , Richard J. Fox , Gjalt W. Huisman , Vesna Gavrilovic , Lisa Marie Newman
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
摘要翻译： 本发明涉及新型卤代醇脱卤素酶多肽和编码它们的多核苷酸。 这些多肽可用于制备4-取代-3-丁酸衍生物和连位氰基羟基取代的羧酸酯。 本发明还提供相关载体，宿主细胞和方法。
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公开(公告)号：US07824898B2
公开(公告)日：2010-11-02
申请号：US10917179
申请日：2004-08-11
申请人： S. Christopher Davis , Richard John Fox , Gjalt W. Huisman , Vesna Gavrilovic , Lisa Marie Newman
发明人： S. Christopher Davis , Richard John Fox , Gjalt W. Huisman , Vesna Gavrilovic , Lisa Marie Newman
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
摘要翻译： 本发明涉及新型卤代醇脱卤素酶多肽和编码它们的多核苷酸。 这些多肽可用于制备4-取代-3-丁酸衍生物和连位氰基羟基取代的羧酸酯。 本发明还提供相关载体，宿主细胞和方法。
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公开(公告)号：US20100028972A1
公开(公告)日：2010-02-04
申请号：US12576195
申请日：2009-10-08
CPC分类号： C12N9/0006
摘要： The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.
摘要翻译： 本发明涉及具有增强的酮还原酶活性和/或用于酮的立体特异性还原的热稳定性的变体多肽。 此外，本发明涉及编码酮还原酶多肽的多核苷酸，包括提供宿主细胞中增强表达的多核苷酸的密码子优化形式。 在另一方面，本发明涉及用本发明的多核苷酸转化的核苷酸构建体，载体和宿主细胞。
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公开(公告)号：US08252554B2
公开(公告)日：2012-08-28
申请号：US12505374
申请日：2009-07-17
申请人： S. Christopher Davis , Lisa Marie Newman , Vesna Gavrilovic , Gjalt W. Huisman , Richard John Fox , Emily C. Mundorff
发明人： S. Christopher Davis , Lisa Marie Newman , Vesna Gavrilovic , Gjalt W. Huisman , Richard John Fox , Emily C. Mundorff
摘要： The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.
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公开(公告)号：US07833767B2
公开(公告)日：2010-11-16
申请号：US12576195
申请日：2009-10-08
CPC分类号： C12N9/0006
摘要： The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.
摘要翻译： 本发明涉及具有增强的酮还原酶活性和/或用于酮的立体特异性还原的热稳定性的变体多肽。 此外，本发明涉及编码酮还原酶多肽的多核苷酸，包括提供宿主细胞中增强表达的多核苷酸的密码子优化形式。 在另一方面，本发明涉及用本发明的多核苷酸转化的核苷酸构建体，载体和宿主细胞。
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