公开(公告)号：US20220068598A1

公开(公告)日：2022-03-03

申请号：US17417451

申请日：2019-12-26

申请人： SANYU ELECTRON CO., LTD. ,  KEIO UNIVERSITY

发明人： Shinsuke SHIBATA ,  Tomoko SHINDO ,  Hideyuki OKANO ,  Shuichi GOTO

IPC分类号： H01J37/28 ,  G01N1/28 ,  H01J37/32

摘要： Sample preparation system and method which enable electron microscope observation of a sample slice with simple structure and process are provided. The sample preparation system includes at least one of a plasma treatment apparatus and a sputtering apparatus, as well as a slice collecting apparatus. The plasma treatment apparatus is configured to feed a resin tape in a plasma irradiation area to irradiate the resin tape with plasma, thereby continuously hydrophilizing the resin tape. The sputtering apparatus is configured to feed the resin tape in a sputtering area to continuously perform sputtering on the resin tape, thereby imparting conductivity to the resin tape. The slice collecting apparatus is configured to serially collect slices cut out from a sample onto the resin tape having been subjected to plasma treatment or sputtering.

公开(公告)号：US20230390459A1

公开(公告)日：2023-12-07

申请号：US18034645

申请日：2021-10-28

申请人： KEIO UNIVERSITY ,  Jiksak Bioengineering Inc.

发明人： Shinsuke SHIBATA ,  Hiroo KIMURA ,  Takayuki NISHIJIMA ,  Tomoko SHINDO ,  Hideyuki OKANO ,  Jiro KAWADA ,  Sayaka TOITA

IPC分类号： A61L27/38

CPC分类号： A61L27/3878 ,  A61L27/3895 ,  A61L27/383

摘要： The present invention relates to an axon bundle obtained by a method of culturing a nerve cell aggregation having one spheroid and an axon bundle extending from the spheroid



wherein the nerve cell aggregation consisting of a plurality of neurons having a cell body and an axon obtained by a method comprising the following step:
supplying a culture medium to one first chamber, one second chamber and one channel having the length of at least 1 mm, the width of 100 to 150 μm and the height of 100 to 200 μm, which connecting said first chamber and second chamber, wherein said first chamber, second chamber, and channel are contained in one of the modules disposed in a culture plate,
seeding the first chamber with a nerve cell derived from a stem cell or a spheroid of primary cultured nerve cells; and
culturing said nerve cell, thereby growing an axon bundle from said spheroid and extending them into said channel,
wherein the length of the axon bundle obtained is 1 mm or more, and the diameter of the axon bundles is 50 μm or more.