公开(公告)号：US09962468B2

公开(公告)日：2018-05-08

申请号：US13264525

申请日：2010-04-15

申请人： Firouzeh Sabri

发明人： Firouzeh Sabri

IPC分类号： A61L27/56 ,  A61L27/38 ,  C12M1/12 ,  C12M1/42 ,  C12N5/00

CPC分类号： A61L27/56 ,  A61L27/383 ,  C12M25/00 ,  C12M35/08 ,  C12N5/0068 ,  C12N2535/10

摘要： This invention describes a cell growth apparatus, particularly neuronal printed circuit board apparatus comprising an aerogel base and a pre-printed cellular growth pattern. The cellular growth pattern is comprised of combinations of layers of cellular adhesion promoting materials, cellular adhesion inhibiting materials, and/or cellular signal promoting materials. The invention further describes methods of promoting cell growth using the neuronal printed circuit board apparatus of the invention. The invention is useful for regeneration and precise guidance of cells, particularly nerve cells, when used as an implant.

公开(公告)号：US20170313981A1

公开(公告)日：2017-11-02

申请号：US15521387

申请日：2015-10-23

申请人： SUMITOMO DAINIPPON PHARMA CO., LTD. ,  RIKEN ,  SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Atsushi KUWAHARA ,  Suguru YAMASAKI ,  Yasushi HIRAMINE ,  Yoshiki SASAI ,  Masayo TAKAHASHI

IPC分类号： C12N5/079 ,  A61L27/38 ,  A61K35/30 ,  G01N33/50 ,  C12N5/0793

CPC分类号： C12N5/0621 ,  A61K35/30 ,  A61K35/44 ,  A61L27/00 ,  A61L27/383 ,  A61L27/3895 ,  C12N5/0619 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2501/50 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/52 ,  C12Q1/02 ,  G01N33/5014 ,  G01N33/5058

摘要： The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3): (1) a first step of culturing human pluripotent stem cells in the absence of feeder cells and in a medium comprising a factor for maintaining undifferentiated state, (2) a second step of culturing the pluripotent stem cells obtained in the first step in suspension in the presence of a Sonic hedgehog signal transduction pathway activating substance to form a cell aggregate, and (3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a 1) a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.

公开(公告)号：US09803167B2

公开(公告)日：2017-10-31

申请号：US14950710

申请日：2015-11-24

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Nakwon Choi ,  Eun-Mi Hur ,  Sohyun Kim ,  Sun-Kyoung Im ,  Eui Sung Yoon ,  Changjoon Justin Lee

IPC分类号： B29C55/00 ,  C12M1/42 ,  B29C55/02 ,  B29C55/20 ,  B29C55/12 ,  C08J5/00 ,  A61L27/16 ,  A61L27/38 ,  A61L27/50

CPC分类号： C12M35/04 ,  A61L27/165 ,  A61L27/3804 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/50 ,  A61L2430/32 ,  B29C55/02 ,  B29C55/023 ,  B29C55/12 ,  B29C55/20 ,  C08J5/00

摘要： The present disclosure relates to stretching apparatus and method for aligning microfibrils. Specifically, the present disclosure provides an apparatus for aligning microfibrils along a single direction, which includes: a first elastic substrate onto which a composition containing microfibrils is loaded; and a stretching module which stretches the width of the elastic substrate. In accordance with the apparatus the present disclosure, microfibrils or cells may be aligned along a particular direction simply by pulling and then releasing the elastic substrate. The present disclosure is also useful for culturing of the aligned cells because the physiological activity of the cells can be maintained and cytotoxicity can be prevented.

公开(公告)号：US20170135802A1

公开(公告)日：2017-05-18

申请号：US14942714

申请日：2015-11-16

申请人： THE TRUSTEES OF PRINCETON UNIVERSITY

发明人： MICHAEL C. McALPINE ,  BLAKE N. JOHNSON

IPC分类号： A61F2/04 ,  A61L27/22 ,  A61L27/36 ,  A61L27/18 ,  A61L27/52 ,  A61L27/54 ,  A61L27/24 ,  A61L27/20

CPC分类号： A61F2/04 ,  A61B17/1128 ,  A61B34/10 ,  A61B2017/00526 ,  A61B2017/1132 ,  A61B2034/105 ,  A61B2034/108 ,  A61B2090/373 ,  A61F2230/0069 ,  A61F2240/002 ,  A61F2250/0067 ,  A61L27/18 ,  A61L27/20 ,  A61L27/222 ,  A61L27/24 ,  A61L27/3675 ,  A61L27/383 ,  A61L27/3878 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/414 ,  A61L2300/432 ,  A61L2430/32 ,  B29C64/112 ,  C08L71/02 ,  C08L5/08

摘要： The present invention includes biomimetic nerve conduits that can be used as nerve regeneration pathways. The present invention further provides methods of preparing and using biomimetic nerve conduits. The disclosed compositions and methods have a broad range of potential applications, for example replacing a missing or damaged section of a nerve pathway of a mammal.

公开(公告)号：US09623044B2

公开(公告)日：2017-04-18

申请号：US14414994

申请日：2013-07-16

申请人： UNIVERSITE CLAUDE BERNARD LYON I ,  INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE LYON ,  UNIVERSITE JEAN MONNET, SAINT ETIENNE ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) ,  UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)

发明人： Fatiha Nothias ,  Sylvia Soares ,  Laurent David ,  Alexandra Montembault

IPC分类号： A61K31/722 ,  A61L27/20 ,  A61L27/52 ,  A61L27/38 ,  A61L27/50 ,  A61K9/16 ,  A61K45/06

CPC分类号： A61K31/722 ,  A61K9/16 ,  A61K45/06 ,  A61L27/20 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/50 ,  A61L27/52 ,  A61L2400/06 ,  A61L2430/32 ,  C08L5/08

摘要： The present invention relates to chitosan hydrogel microparticles of a median size d50 comprised between 1 and 500 μm (obtained from a number distribution), the chitosan having a degree of acetylation of less than equal to 20% and its concentration in the hydrogel being comprised between 0.25 and 5% by weight based on a total weight of the hydrogel, for use in neuron regeneration and/or in the repair of the nervous system, advantageously of the central nervous system, and/or in the grafting of neurons and/or in the treatment of neurodegenerative diseases and/or in the treatment of paralyses.It also relates to an implant comprising an aqueous suspension of microparticles mixed with Schwann cells and/or stem cells and/or trophic factors.

公开(公告)号：US09597429B2

公开(公告)日：2017-03-21

申请号：US14274156

申请日：2014-05-09

申请人： Board of Regents, The University of Texas System

发明人： Christine E. Schmidt ,  Terry Hudson

IPC分类号： A61L27/36 ,  A61L27/38

CPC分类号： A61L27/3633 ,  A61L27/3604 ,  A61L27/3675 ,  A61L27/3687 ,  A61L27/383 ,  A61L27/3878 ,  A61L2430/40

摘要： The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solutions of the buffered salt to remove excess detergent. The natural, cell-free tissue replacement may be a nerve graft that supports axonal regeneration, guides the axons toward the distal nerve end and/or is immunologically tolerated. Other forms of the invention are a composition and kit prepared by the method of making a native, cell-free tissue replacement. The present invention may be modified for use in diagnostic, therapeutic, and prophylactic applications.

公开(公告)号：US09592255B2

公开(公告)日：2017-03-14

申请号：US13805486

申请日：2011-07-01

申请人： Lisa M. Larkin ,  Ellen M. Arruda ,  Jennifer Baltich ,  Aaron Adams ,  Leah Hatch-Vallier

发明人： Lisa M. Larkin ,  Ellen M. Arruda ,  Jennifer Baltich ,  Aaron Adams ,  Leah Hatch-Vallier

IPC分类号： A61K35/12 ,  A61K35/33 ,  A61K35/30 ,  A61L27/38 ,  C12N5/079 ,  C12N5/071

CPC分类号： A61K35/33 ,  A61K35/30 ,  A61L27/3804 ,  A61L27/383 ,  A61L27/3878 ,  A61L27/3891 ,  A61L2430/32 ,  C12N5/0622 ,  C12N5/0697 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2502/08 ,  C12N2502/086 ,  C12N2502/1323 ,  C12N2506/1307 ,  C12N2506/1384 ,  C12N2533/10 ,  C12N2533/52 ,  A61K2300/00

摘要： The invention relates to scaffold-free three dimensional nerve fibroblast constructs and method of generating the nerve fibroblast constructs. The invention also relates to methods or repairing nerve transection and replacing damaged nerve tissue using the nerve fibroblast constructs of the invention.

摘要翻译： 本发明涉及无支架的三维神经成纤维细胞构建体和产生神经成纤维细胞构建体的方法。 本发明还涉及使用本发明的神经成纤维细胞构建体的方法或修复神经横断并替换受损的神经组织。

公开(公告)号：US09440008B2

公开(公告)日：2016-09-13

申请号：US14694466

申请日：2015-04-23

申请人： Children's Medical Center Corporation ,  Massachusetts Institute of Technology

发明人： Robert S. Langer ,  Rajiv Saigal ,  Yang Teng

IPC分类号： A61F13/00 ,  A61L31/06 ,  A61K9/00 ,  A61F2/02 ,  A61L31/14 ,  A61L27/26 ,  A61L27/58 ,  A61L31/16 ,  A61F2/44 ,  A61L31/00 ,  A61L31/04

CPC分类号： A61L27/50 ,  A61F2/02 ,  A61F2/442 ,  A61F2210/0004 ,  A61K9/0085 ,  A61L27/18 ,  A61L27/26 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/3878 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L31/005 ,  A61L31/048 ,  A61L31/06 ,  A61L31/14 ,  A61L31/146 ,  A61L31/148 ,  A61L31/16 ,  A61L2300/41 ,  A61L2300/412 ,  A61L2300/414 ,  A61L2300/60 ,  A61L2300/606 ,  A61L2300/64 ,  A61L2300/802 ,  A61L2400/00 ,  A61L2430/38 ,  C08L67/04

摘要： Devices and methods for the treatment of open and closed wound spinal cord injuries are disclosed. For example, described herein are devices and methods for mitigating secondary injury to, and promoting recovery of, spinal cord primary injuries. More particularly, certain embodiments of the present invention are directed to polymeric mini-tubes that may be used for the treatment of spinal cord injuries. In addition, other embodiments are directed to polymeric “fill-in” bandages that may be used for the treatment of spinal cord injuries. For example, an erodible, or biodegradable, form of biocompatible polymer of the present invention is fabricated for surgical implantation into the site of the spinal cord injury.

公开(公告)号：US20160152946A1

公开(公告)日：2016-06-02

申请号：US14950710

申请日：2015-11-24

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Nakwon CHOI ,  Eun-Mi HUR ,  Sohyun KIM ,  Sun-Kyoung IM ,  Eui Sung YOON ,  Changjoon Justin LEE

IPC分类号： C12N5/00

CPC分类号： C12M35/04 ,  A61L27/165 ,  A61L27/3804 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/50 ,  A61L2430/32 ,  B29C55/02 ,  B29C55/023 ,  B29C55/12 ,  B29C55/20 ,  C08J5/00

摘要： The present disclosure relates to stretching apparatus and method for aligning microfibrils. Specifically, the present disclosure provides an apparatus for aligning microfibrils along a single direction, which includes: a first elastic substrate onto which a composition containing microfibrils is loaded; and a stretching module which stretches the width of the elastic substrate. In accordance with the apparatus the present disclosure, microfibrils or cells may be aligned along a particular direction simply by pulling and then releasing the elastic substrate. The present disclosure is also useful for culturing of the aligned cells because the physiological activity of the cells can be maintained and cytotoxicity can be prevented.

摘要翻译： 本发明涉及用于对准微纤丝的拉伸装置和方法。 具体地说，本公开内容提供了一种沿着单一方向对准微原纤维的装置，其包括：第一弹性基材，其上装载有微原纤维的组合物; 以及延伸弹性基板的宽度的拉伸模块。 根据本公开的装置，微原纤维或细胞可以简单地通过拉动然后释放弹性基底沿着特定方向排列。 本公开也可用于培养对准的细胞，因为可以保持细胞的生理活性并且可以防止细胞毒性。

公开(公告)号：US09309494B2

公开(公告)日：2016-04-12

申请号：US12981581

申请日：2010-12-30

申请人： Li Fan ,  Chen Feng ,  Wen-Mei Zhao

发明人： Li Fan ,  Chen Feng ,  Wen-Mei Zhao

IPC分类号： C12N5/079 ,  C12N5/0793 ,  A61L27/34 ,  A61L27/38

CPC分类号： C12N5/0619 ,  A61L27/34 ,  A61L27/383 ,  A61L27/3878 ,  A61L2400/12 ,  A61L2430/32 ,  C12N2533/20 ,  C12N2533/32 ,  C12N2533/50 ,  C12N2535/00 ,  C08L89/00

摘要： A method for making a nerve graft includes the following steps. A culture layer including a carbon nanotube film structure and a protein layer is provided. The protein layer is located on a surface of the carbon nanotube film structure. A number of nerve cells are seeded on a surface of the protein layer away from the carbon nanotube film structure. The nerve cells are cultured until a number of neurites branch from the nerve cells and are connected between the nerve cells.

摘要翻译： 制造神经移植物的方法包括以下步骤。 提供了包括碳纳米管膜结构和蛋白质层的培养层。 蛋白质层位于碳纳米管膜结构的表面上。 将多个神经细胞接种在远离碳纳米管膜结构的蛋白质层的表面上。 将神经细胞培养直到许多神经突从神经细胞分支并连接在神经细胞之间。