公开(公告)号：US12090251B2

公开(公告)日：2024-09-17

申请号：US17049867

申请日：2019-04-25

申请人： Sapporo Medical University

发明人： Takako Chikenji ,  Mineko Fujimiya ,  Yuki Saito ,  Masako Nakano ,  Naoto Konari ,  Miho Otani

IPC分类号： A61L27/38 ,  A61K35/28 ,  A61L27/56 ,  A61P13/12 ,  A61P25/28 ,  C12M3/00 ,  C12N5/0775

CPC分类号： A61L27/3834 ,  A61K35/28 ,  A61L27/3878 ,  A61L27/56 ,  A61P13/12 ,  A61P25/28 ,  C12M21/08 ,  C12N5/0663 ,  C12N5/0667 ,  A61L2300/64 ,  A61L2430/26 ,  A61L2430/32

摘要： A cell sheet for transplantation into a living body, containing MSCs having an average cell density of 3.0×104 cells/cm2 or less on the surface of the sheet is provided. A method for producing a cell sheet for transplantation into a living body, including: a step of seeding MSCs on a cell culture carrier having a three-dimensional structure formed of fibers at a cell number of 3.0×105 cells/cm2 or less; and a step of culturing the MSCs and thereby preparing a cell sheet containing the MSCs having an average cell density of 3.0×104 cells/cm2 or less is also provided.

公开(公告)号：US11998661B2

公开(公告)日：2024-06-04

申请号：US17451489

申请日：2021-10-20

申请人： Axogen Corporation

发明人： Jennifer Faleris ,  Kasra Tajdaran

IPC分类号： A61L27/52 ,  A61L27/20 ,  A61L27/22 ,  A61L27/24 ,  A61L27/38

CPC分类号： A61L27/3878 ,  A61L27/20 ,  A61L27/222 ,  A61L27/225 ,  A61L27/227 ,  A61L27/24 ,  A61L27/52 ,  A61L2430/32

摘要： The present disclosure provides fasciculated nerve grafts of customizable lengths and diameters, and methods of preparing the same. The grafts are made by digesting native extracellular matrix (ECM) around the nerve fascicles of a nerve tissue, and the epineurial sheath. One or more of the individual fascicles may then be entubulated in an entubulation material, embedded in or coated with a coating material, or both, to form a fasciculated nerve graft. The fasciculated nerve grafts are customizable and designed to bridge nerve gaps; the modularity of the fasciculated nerve graft allows for restoring continuity to nerve defects of virtually any length and allows for matching the diameter of the patient's recipient nerve.

公开(公告)号：US11944721B2

公开(公告)日：2024-04-02

申请号：US18305346

申请日：2023-04-22

申请人： Shanghai Sixth People's Hospital

发明人： Yun Qian ,  Cunyi Fan ,  Xiangyun Yao ,  Zhiwen Yan ,  Lingchi Kong ,  Xu Wang

IPC分类号： A61L27/38 ,  A61L27/44

CPC分类号： A61L27/3878 ,  A61L27/383 ,  A61L27/443 ,  A61L2430/32

摘要： A nerve conduit loaded with adipose-derived stem cells and a preparation method thereof are provided. The preparation method includes: S1, adding polycaprolactone and polyvinylpyrrolidone into a binary organic solvent, performing ultrasonic treatment, and then adding reduced graphene oxide nanoparticles to obtain a spinning solution; S2, electrospinning with the spinning solution and then washing for several times to obtain a semi-finished conduit product; and S3, injecting a cell mixture into the semi-finished conduit product to obtain the nerve conduit. A fiber surface of the nerve conduit has groove structures, and thus a specific surface area and cell adhesion sites are increased, and adhesion and proliferation of cells are facilitated. By loading the adipose-derived stem cells, neurotrophic phenotypic effect of peripheral nerve scaffold is improved, and can effectively avoid immunological rejection of transplantation, promote orientational growth of axons into the nerve conduit and promote myelination effect of Schwann cells.

公开(公告)号：US11896738B2

公开(公告)日：2024-02-13

申请号：US17198211

申请日：2021-03-10

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Rebecca Bierman

IPC分类号： A61L27/52 ,  A61L27/44 ,  A61L27/34 ,  C08K3/04 ,  A61N1/05 ,  C08K7/06 ,  C08J3/075 ,  A61L27/38 ,  C08K7/24

CPC分类号： A61L27/52 ,  A61L27/34 ,  A61L27/3834 ,  A61L27/3878 ,  A61L27/443 ,  A61N1/0551 ,  C08J3/075 ,  C08K3/042 ,  A61L2400/12 ,  A61L2400/18 ,  C08J2305/08 ,  C08K7/06 ,  C08K7/24 ,  C08K2201/001

摘要： An electrically conductive hyaluronic acid-based hydrogel is disclosed that is a crosslinked porous scaffold having a graphene-based material encapsulated or in contact within the porous scaffold. The graphene-based material includes one or more of graphene oxide foam, reduced graphene oxide foam, nanoplatelets, nanoparticles, or fibers. The porous scaffold may be formed over an implanted bioelectronic device such as a microelectrode array having a plurality of electrodes. The porous scaffold may also be used to control the differentiation of cells including Neural Stem/Progenitor Cells (NS/PCs).

公开(公告)号：US20190192736A1

公开(公告)日：2019-06-27

申请号：US16260733

申请日：2019-01-29

申请人： MiMedx Group, Inc.

发明人： Brenda S. Morse ,  Somaly Sith ,  Randall Spencer ,  John Daniel

IPC分类号： A61L27/38 ,  A61L27/36 ,  A61K35/50

CPC分类号： A61L27/3891 ,  A61K35/50 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3878 ,  A61L27/3882 ,  A61L2400/18 ,  A61L2430/12 ,  A61L2430/16 ,  A61L2430/22 ,  A61L2430/32 ,  A61L2430/40

摘要： Described herein are tissue grafts derived from the placenta that possess good adhesion to biological tissues and are useful in would healing applications. In one aspect, the tissue graft includes (1) two or more layers of amnion, wherein at least one layer of amnion is cross-linked, (2) two or more layers of chorion, wherein at least one layer of amnion is cross-linked, or (3) one or more layers of amnion and chorion, wherein at least one layer of amnion and/or chorion is cross-linked. In another aspect, the grafts are composed of amnion and chorion cross-linked with one another. In a further aspect, the grafts have one or more layers sandwiched between the amnion and chorion membranes. The amnion and/or the chorion are treated with a cross-linking agent prior to the formation of the graft. The presence of the cross-linking agent present on the graft also enhances adhesion to the biological tissue of interest. Also described herein are methods for making and using the tissue grafts.

公开(公告)号：US20180105795A1

公开(公告)日：2018-04-19

申请号：US15730770

申请日：2017-10-12

申请人： SUN YAT-SEN UNIVERSITY

发明人： Yuan-shan Zeng ,  Ge Li ,  Jia-hui Sun

IPC分类号： C12N5/079 ,  A61L27/36 ,  A61L27/38 ,  A61K35/12

CPC分类号： C12N5/0618 ,  A61K35/12 ,  A61K35/30 ,  A61L27/3604 ,  A61L27/3633 ,  A61L27/3675 ,  A61L27/3683 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3878 ,  A61L27/54 ,  A61L2300/414 ,  A61L2430/32 ,  C12N5/062 ,  C12N2533/90

摘要： An extracellular matrix-modified decellularized nerve scaffold and use thereof are provided. The extracellular matrix-modified decellularized nerve scaffold is prepared from a natural porcine optic nerve. The scaffold has a plurality of longitudinal channels and a plurality of transversal foramina intercommunicated with the longitudinal channels, which have relatively uniform diameters and relatively even distributions in the scaffold. The extracellular matrix-modified decellularized optic nerve scaffold of the present invention changes the poor microenvironment of existing decellularized material that lacks cell growth factors and nutrients, supports seeded cells to form neural networks in vitro or in vivo, and enables the connection of ascending nerve fibers or descending nerve fibers of the injured spinal cord to their target cells after transplantation.

公开(公告)号：US20170296193A1

公开(公告)日：2017-10-19

申请号：US15638317

申请日：2017-06-29

申请人： The Johns Hopkins University

发明人： Ahmet Hoke ,  Shawn H. Lim ,  Xingyu Liu ,  Hai-Quan Mao

IPC分类号： A61B17/11 ,  A61F2/00 ,  A61F2/02 ,  A61L27/38 ,  A61L27/52 ,  A61L27/48 ,  A61L27/56 ,  A61L27/54 ,  A61L27/58

CPC分类号： A61B17/1128 ,  A61F2/0063 ,  A61F2/02 ,  A61L27/383 ,  A61L27/3878 ,  A61L27/48 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/414 ,  A61L2300/602 ,  A61L2300/608 ,  A61L2400/12 ,  A61L2430/32 ,  Y10T156/10 ,  Y10T156/1043

摘要： The present invention is directed to the compositions and methods of preparing hydrogel-grafted nerve guides for peripheral nerve regeneration. Particularly, the present invention describes the nerve guides and methods for preparation of hydrogel-grafted nerve guides with encapsulated neurotrophic factors and a nanofiber mesh lining the inner surface of the guide. The present invention also provides methods for peripheral nerve repair using these hydrogel-grafted nerve guides.

公开(公告)号：US20170065745A1

公开(公告)日：2017-03-09

申请号：US15357539

申请日：2016-11-21

申请人： DePuy Synthes Products, Inc.

发明人： Laura J. Brown ,  Anna Gosiewska ,  Anthony J. Kihm ,  Brian C. Kramer

IPC分类号： A61L27/38 ,  A61L27/52 ,  A61L27/22 ,  A61L27/54

CPC分类号： A61L27/3878 ,  A61K35/12 ,  A61K35/50 ,  A61K35/51 ,  A61K38/1774 ,  A61K38/1841 ,  A61K38/1858 ,  A61L27/225 ,  A61L27/227 ,  A61L27/3834 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/414 ,  A61L2400/06 ,  A61L2430/38 ,  C12N5/0605

摘要： Methods for treating a patient having a disease or condition related to IVD degeneration are provided. The methods comprise administering cells obtained from human umbilical cord tissue, or administering pharmaceutical compositions comprising such cells or prepared from such cells and optionally a hydrogel. In some embodiments, administering the cells promotes repair and regeneration of degenerated IVD tissue in the patient. Pharmaceutical compositions for use in the inventive methods, as well as kits for practicing the methods are also provided.

摘要翻译： 提供了治疗患有与IVD变性相关的疾病或病症的患者的方法。 所述方法包括施用从人脐带组织获得的细胞，或施用包含此类细胞的药物组合物或由此类细胞制备，以及任选的水凝胶。 在一些实施方案中，施用细胞促进患者中退化的IVD组织的修复和再生。 还提供了用于本发明方法的药物组合物，以及用于实施该方法的试剂盒。

公开(公告)号：US09428565B2

公开(公告)日：2016-08-30

申请号：US13982343

申请日：2012-01-31

申请人： Khalid Shah

发明人： Khalid Shah

IPC分类号： C07K14/00 ,  A61K38/02 ,  A61K45/00 ,  C07K14/47 ,  A61K38/17 ,  A61K35/28 ,  A61K35/30 ,  A61K39/395 ,  A61K45/06

CPC分类号： C07K14/4747 ,  A01K2207/12 ,  A61K35/28 ,  A61K35/30 ,  A61K38/1761 ,  A61K38/1774 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/36 ,  A61L27/383 ,  A61L27/3834 ,  A61L27/3878 ,  A61L27/44 ,  A61L27/54 ,  A61L27/58 ,  A61L2300/416 ,  A61L2300/64 ,  C07K2319/55 ,  C07K2319/61 ,  C12N9/1205 ,  C12N15/86 ,  C12N2740/15043 ,  C12Y207/01145

摘要： Described herein are novel compositions comprising multimodal TRAIL agents and cells engineered to express such multimodal TRAIL agents, including cells encapsulated in a scaffold or matrix, for use in the treatment of disorders such as cancer.

摘要翻译： 本文描述的是包含多峰TRAIL试剂和工程化以表达这种多峰TRAIL试剂（包括包封在支架或基质中的细胞），用于治疗病症如癌症的细胞的新型组合物。

公开(公告)号：US20160045641A1

公开(公告)日：2016-02-18

申请号：US14826927

申请日：2015-08-14

申请人： NATIONAL DEFENSE MEDICAL CENTER

发明人： Chung-Hsing CHOU ,  Dueng-Yuan Hueng ,  Tsung-Neng TSAI

IPC分类号： A61L27/38 ,  C12N5/071 ,  A61L27/24 ,  A61L27/50 ,  A61L27/36

CPC分类号： A61L27/3891 ,  A61K35/30 ,  A61K35/44 ,  A61L27/24 ,  A61L27/3633 ,  A61L27/3675 ,  A61L27/3808 ,  A61L27/383 ,  A61L27/3878 ,  A61L2430/32 ,  C12N5/0618 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/392 ,  C12N2502/088 ,  C12N2502/28 ,  C12N2533/54 ,  A61K2300/00

摘要： The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.

摘要翻译： 本发明涉及用于神经血管重建的细胞片构建体。 细胞片构建体具有血管内皮细胞层和神经干细胞层，并且两层物理上彼此直接接触，其中血管内皮细胞层形成分支血管系统，神经干细胞层分化为神经元。 本发明还涉及一种用于制造细胞片构建体的方法，具有以下步骤：在基底上培养血管内皮细胞以形成血管内皮细胞层，在血管内皮细胞层上接种神经干细胞以制备神经干细胞 物理上与血管内皮细胞层直接接触，并培养神经干细胞和血管内皮细胞层，以分化为神经元和分支血管系统以形成细胞片构建体。