公开(公告)号：US20200181486A1

公开(公告)日：2020-06-11

申请号：US16329095

申请日：2017-08-31

Applicant: SEKISUI CHEMICAL CO., LTD. ,  SEKISUI MEDICAL CO., LTD.

Inventor： Tadashi IWAMOTO ,  Satoru SUGIMOTO ,  Takeshi WAKIYA ,  Shinichiro KITAHARA ,  Maasa YAJI

IPC: C09K11/06 ,  G01N33/58 ,  G01N33/533 ,  A61K49/00 ,  G01N33/543 ,  C09K11/02

Abstract: The present invention provides fluorescent particles for a diagnostic agent which contain a synthetic polymer and at least 10 mass %, on a total particle mass basis, of an aggregation-induced emission material, and have on the surface thereof a binding partner which binds with an analyte. These fluorescent particles for a diagnostic agent are non-toxic, have improved visibility and reproducibility, and support a binding partner for an analyte.

公开(公告)号：US20180282491A1

公开(公告)日：2018-10-04

申请号：US15764801

申请日：2016-09-30

Applicant: SEKISUI CHEMICAL CO., LTD. ,  SEKISUI MEDICAL CO., LTD.

Inventor： Satoru SUGIMOTO ,  Tadashi IWAMOTO ,  Takeshi WAKIYA ,  Shinichiro KITAHARA ,  Maasa IKEGAMI

IPC: C08J3/14 ,  C08L9/06 ,  G01N33/545

Abstract: A polymer fine particle including a block copolymer having two or more segments, wherein the polymer fine particle has a microphase separated structure on a surface thereof, the number-average particle size is 50 nm to 1000 nm, and the coefficient of variation is less than 20%. The polymer fine particle permits highly sensitive assay by controlling the antigen- or antibody-immobilized state of the particle surface.

公开(公告)号：US20200080996A1

公开(公告)日：2020-03-12

申请号：US16616371

申请日：2018-05-24

Applicant: SEKISUI CHEMICAL CO., LTD. ,  SEKISUI MEDICAL CO., LTD.

Inventor： Satoru SUGIMOTO ,  Takeshi WAKIYA ,  Shinichiro KITAHARA ,  Maasa YAJI ,  Yuya INABA

IPC: G01N33/546 ,  C08F12/32 ,  C08F12/08 ,  C08F22/20 ,  C08F28/06

Abstract: The present invention relates to latex particles for measurement reagents, said latex particles having a coefficient of variation in particle diameters of 10% or less and an average particle diameter of 250 to 1000 nm. Each of the latex particles contains 20% by weight or more of a compound having a refractive index of 1.60 or more. The depth of a supernatant is 5 mm or less when a liquid dispersion having a solid matter concentration of 1% by weight, which is prepared by dispersing the latex particles in ultrapure water, is placed in a tubular 10-ml measuring cylinder having a body inner diameter of 10.8 mm and is then allowed to leave for 10 days. According to the particles, a measurement of a measurement sample can be performed at a high sensitivity even when the concentration of a substance to be tested in the measurement sample is low.

公开(公告)号：US20190226988A1

公开(公告)日：2019-07-25

申请号：US16329116

申请日：2017-08-31

Applicant: SEKISUI CHEMICAL CO., LTD. ,  SEKISUI MEDICAL CO., LTD.

Inventor： Tadashi IWAMOTO ,  Satoru SUGIMOTO ,  Takeshi WAKIYA ,  Shinichiro KITAHARA ,  Maasa YAJI

IPC: G01N21/64 ,  G01N33/543

Abstract: This analyte concentration measuring method including: preparing a mixed solution by mixing a sample solution containing an analyte, with a solution containing aggregation-induced emission fluorescent material-containing particles that have a binding partner which binds with the analyte and that agglutinate and fluoresce when the analyte binds to the binding partner; measuring the fluorescence intensity generated from the aggregation-induced emission fluorescent material-containing particles in the mixed solution; and comparing a fluorescence intensity calibration curve for analyte concentration with the fluorescence intensity, and associating the fluorescence intensity with the analyte concentration in the mixed solution. Employing agglutinating-luminescent-material-containing particles enables measurements to be carried out with a satisfactory detection sensitivity while suppressing background fluorescence.

公开(公告)号：US20160011181A1

公开(公告)日：2016-01-14

申请号：US14865728

申请日：2015-09-25

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Yuki TAKAHASHI ,  Tomo SHIMIZU ,  Yasushi NAKAMURA ,  Shinya NAKAYAMA ,  Shinichiro KITAHARA

IPC: G01N33/543 ,  G01N33/573 ,  G01N33/577 ,  G01N33/574

CPC classification number: G01N33/543 ,  G01N33/573 ,  G01N33/57434 ,  G01N33/577 ,  G01N2333/96433

Abstract: Provided is a method for the simple and highly accurate assay of PSA by a one-step reaction that does not use carriers having different average grain sizes. Also provided is a reagent therefor. The PSA assay method comprises sensitizing insoluble carriers having the same average grain size within a range of greater than 0.20 nm but 0.40 nm or less using two types of anti-PSA monoclonal antibodies having different epitopes that are anti-PSA monoclonal antibodies that will react with both free PSA and PSA-ACT, which is a complex of free PSA and α1-antichymotrypsin, and bringing the carriers into contact with a sample in the presence of an aggregation promoter.

Abstract translation: 提供了通过不使用具有不同平均粒径的载体的一步反应简单且高度精确地测定PSA的方法。 还提供了一种试剂。 PSA测定方法包括使用具有不同表位的两种抗PSA单克隆抗体对具有相同平均粒径的不溶性载体进行敏化，所述不同载体是具有不同表位的抗PSA单克隆抗体，所述抗PSA单克隆抗体是将与 游离PSA和PSA-ACT两者均为游离PSA和α1-抗胰凝乳蛋白酶的复合物，并使载体在聚集促进剂存在下与样品接触。

公开(公告)号：US20220187287A1

公开(公告)日：2022-06-16

申请号：US17598015

申请日：2020-03-25

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Maasa YAJI ,  Yuya INABA ,  Shinichiro KITAHARA ,  Mitsuaki YAMAMOTO ,  Takeshi WAKIYA ,  Mai YAMAGAMI ,  Shuhei OBINATA

IPC: G01N33/543 ,  G01N33/553

Abstract: Disclosed is a sensitized magnetic responsive particle including: a magnetic responsive particle having a core particle and at least one magnetic layer that is disposed on the core particle and includes microparticles of a magnetic metal and/or an oxide thereof; and a substance that is supported on the magnetic responsive Particle and interacts specifically with an analyte, wherein the coefficient of variation in the weight-average particle size of the magnetic responsive particles is 15% or less. The magnetic responsive particle provided has excellent magnetic separability.

公开(公告)号：US20220170917A1

公开(公告)日：2022-06-02

申请号：US17436794

申请日：2020-03-06

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Ei-ichiro SUNAMURA ,  Osamu MIYAZAKI ,  Takuya YOTANI ,  Shinichiro KITAHARA ,  Manami IWASAKI

IPC: G01N33/53

Abstract: A problem to be solved is to perform an immunoassay method for BG in a biological sample having a sensitivity equivalent to and a reactivity similar to a Limulus amebocyte lysate reagent. The problem can be solved by an immunoassay method for β-D-glucan in a biological sample, comprising assaying β-D-glucan in the biological sample by using a monoclonal antibody binding to (1→3)-β-D-glucan having a degree of polymerization of 4.

公开(公告)号：US20210396741A1

公开(公告)日：2021-12-23

申请号：US17292588

申请日：2019-11-08

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Tadaaki YOSHIDA ,  Chihiro MIZUNIWA ,  Shinichiro KITAHARA ,  Hiroshi TAKAHASHI

IPC: G01N33/53 ,  G01N21/77

Abstract: There is provided a method for preventing an occurrence of an irregular detection value and performing measurement with good reproducibility in an immunoassay using an automatic analyzer regardless of whether the reaction cuvette used is of a disposable type or a reusable type and whether the optical detection method used measures transmitted light or scattered light. There is also provided an immunoassay reagent for use in the method. A method for preventing the occurrence of irregular detection and enhancing the reproducibility of measurement includes using a reagent containing polyethylene glycol or the like having a weight-average molecular weight of 300 to 3000.

公开(公告)号：US20230118882A1

公开(公告)日：2023-04-20

申请号：US17914044

申请日：2021-03-24

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Maasa YAJI ,  Yuya INABA ,  Shinichiro KITAHARA ,  Takeshi WAKIYA ,  Mai YAMAGAMI ,  Shuhei OBINATA

IPC: G01N33/553

Abstract: Disclosed is a sensitized magnetic responsive particle including: a magnetic responsive particle having a core particle and at least one magnetic layer disposed on the core particle, the magnetic layer containing microparticles of a magnetic metal and/or an oxide thereof; and a substance that specifically interacts with an analyte, the substance being supported on the magnetic responsive particle, wherein, assuming that a volume and a weight of the core particle are respectively vc and wc, and that a volume and a weight of the magnetic responsive particle are respectively ve and we, the magnetic material density [(we−wc)/(ve−vc)] satisfies the following expression 1: 2.0≤(we−wc)/(ve−vc)   Expression 1 The magnetic responsive particle has a high magnetic collection property in spite of a small particle size. When the magnetic responsive particle is used, a reagent for an immunoassay having excellent magnetic separability and capable of realizing high sensitivity can be provided.

公开(公告)号：US20140295576A1

公开(公告)日：2014-10-02

申请号：US14306556

申请日：2014-06-17

Applicant: Sekisui Medical Co., Ltd.

Inventor： Takayuki AKAMINE ,  Shinichiro KITAHARA ,  Tetsuya OTA ,  Takayuki ABE

IPC: G01N33/543

CPC classification number: G01N33/54313 ,  G01N33/544 ,  G01N33/6854

Abstract: The present invention has an object to provide an insoluble carrier for an antiphospholipid antibody detection reagent having a high reactivity. The present invention also has an object to provide an antiphospholipid antibody detection reagent, and a method of detecting an antiphospholipid antibody. The present invention directs to an insoluble carrier for an antiphospholipid antibody detection reagent, having a zeta potential of lower than −45 mV in the case that the insoluble carrier is suspended in a 20 mmol/L aqueous sodium phosphate solution with a pH of 7.4 so that the resulting suspension has a solids concentration of 0.1%.

Abstract translation: 本发明的目的是提供具有高反应性的抗磷脂抗体检测试剂的不溶性载体。 本发明的目的还在于提供抗磷脂抗体检测试剂和检测抗磷脂抗体的方法。 在不溶性载体悬浮于pH7.4的20mmol / L磷酸钠水溶液的情况下，本发明指出了抗磷脂抗体检测试剂的不溶性载体，其具有低于-45mV的ζ电位 所得悬浮液的固体浓度为0.1％。