公开(公告)号：US09617563B2

公开(公告)日：2017-04-11

申请号：US14429510

申请日：2013-09-19

申请人： SEKISUI CHEMICAL CO., LTD. ,  TOHOKU UNIVERSITY

发明人： Masahiro Furutani ,  Akihiro Uenishi ,  Koichiro Iwasa ,  Yasuyuki Kori ,  Seiji Takahashi ,  Takefumi Shimoyama

IPC分类号： C12P5/00 ,  C12N1/20 ,  C12N15/00 ,  C12N1/14 ,  C12N9/10 ,  C12N9/88 ,  C07H21/04 ,  C12N15/52 ,  C12N9/00

CPC分类号： C12P5/007 ,  C12N9/00 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/52 ,  C12Y205/01001 ,  C12Y205/01028 ,  C12Y402/03051

摘要： To provide a series of techniques for obtaining β-phellandrene with high purity and in a large quantity.Provided is a recombinant cell capable of producing β-phellandrene, prepared by introducing at least one nucleic acid selected from the group consisting of a nucleic acid encoding geranyl pyrophosphate (GPP) synthase and a nucleic acid encoding neryl pyrophosphate (NPP) synthase, and a nucleic acid encoding β-phellandrene synthase into a host cell in such a manner that these nucleic acids are expressed in the host cell. Also provided is a method for producing β-phellandrene by culturing the recombinant cell to produce β-phellandrene in the recombinant cell.

公开(公告)号：US20150218589A1

公开(公告)日：2015-08-06

申请号：US14429510

申请日：2013-09-19

申请人： SEKISUI CHEMICAL CO., LTD. ,  TOHOKU UNIVERSITY

发明人： Masahiro Furutani ,  Akihiro Uenishi ,  Koichiro Iwasa ,  Yasuyuki Kori ,  Seiji Takahashi ,  Takefumi Shimoyama

IPC分类号： C12P5/00 ,  C12N9/88 ,  C12N9/10

CPC分类号： C12P5/007 ,  C12N9/00 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/52 ,  C12Y205/01001 ,  C12Y205/01028 ,  C12Y402/03051

摘要： To provide a series of techniques for obtaining β-phellandrene with high purity and in a large quantity.Provided is a recombinant cell capable of producing β-phellandrene, prepared by introducing at least one nucleic acid selected from the group consisting of a nucleic acid encoding geranyl pyrophosphate (GPP) synthase and a nucleic acid encoding neryl pyrophosphate (NPP) synthase, and a nucleic acid encoding β-phellandrene synthase into a host cell in such a manner that these nucleic acids are expressed in the host cell. Also provided is a method for producing β-phellandrene by culturing the recombinant cell to produce β-phellandrene in the recombinant cell.

摘要翻译： 提供一系列用于获得高纯度和大量生物化学物质的方法。 提供能够产生通过引入选自由编码香叶基焦磷酸（GPP）合酶的核酸和编码橙色素焦磷酸（NPP）合酶）的核酸组成的组中的至少一种的核酸而制备的重组细胞，以及 以这种方式将宿主细胞中的这些核酸表达在宿主细胞中，将编码β-胡萝卜素合酶的核酸编码到宿主细胞中。 还提供了通过培养重组细胞以在重组细胞中产生并生成β-肾上腺素的方法来生产β-肾上腺素。