公开(公告)号：US20240060980A1

公开(公告)日：2024-02-22

申请号：US18265816

申请日：2021-12-07

Applicant: DAI NIPPON TORYO CO., LTD. ,  SHIMADZU CORPORATION

Inventor： Yuta MIYAZAWA ,  Hirotaka FUJIMOTO ,  Makoto WATANABE ,  Taka-Aki SATO

IPC: G01N33/574 ,  G01N33/543

CPC classification number: G01N33/57415 ,  G01N33/57488 ,  G01N33/54387 ,  G01N2470/04

Abstract: An object of the present invention is to provide a method for detecting particulate substances with a high degree of sensitivity, while suppressing the generation of non-specific signals. According to the present invention, the generation of non-specific signals in the background can be suppressed by blocking a membrane of a test strip which is used for immunochromatography with a blocking composition including a polymer-based blocking agent having, in its side chain, a substituent represented by the following formula:




(wherein R1, R2, and R3 are each independently a hydrogen atom, a C1-6 alkyl group, or a C1-6 hydroxyalkyl group; and n is an integer of 1 to 4), and having a weight average molecular weight (Mw) of 1×103 to 1×107.

公开(公告)号：US20220317128A1

公开(公告)日：2022-10-06

申请号：US17640032

申请日：2020-09-07

Applicant: DAI NIPPON TORYO CO., LTD. ,  SHIMADZU CORPORATION

Inventor： Yuta MIYAZAWA ,  Daigou MIZOGUCHI ,  Hirotaka FUJIMOTO ,  Makoto WATANABE ,  Taka-Aki SATO

IPC: G01N33/574 ,  G01N33/543 ,  G01N21/76 ,  G01N21/64

Abstract: The present invention relates to a method for detecting a particulate substance by immunochromatography, the particulate substance including, on its surface, a plurality of substances to be bound containing a first substance to be bound and a second substance to be bound which may be the same or different with respect to each other, wherein the method includes the steps of: (1) contacting on a membrane a sample containing the particulate substance with a first specific binding substance for the first substance to be bound to capture the particulate substance with the first specific binding substance; (2) contacting the captured particulate substance with a second specific binding substance for the second substance to be bound to label the particulate substance; and (3) detecting the labeled particulate substance, wherein the first specific binding substance is immobilized on the membrane, and the second specific binding substance is bound to a metal nanoparticle.

公开(公告)号：US20220145376A1

公开(公告)日：2022-05-12

申请号：US17611603

申请日：2020-05-13

Applicant: SHIMADZU CORPORATION

Inventor： Makoto WATANABE ,  Hirotaka FUJIMOTO ,  Taka-Aki SATO

IPC: C12Q1/6818 ,  G01N27/62

Abstract: The present invention provides a method for quantifying a plurality of surface antigens simultaneously, thereby identifying an extracellular vesicle such as an exosome, and detecting and quantifying any surface antigen. Specifically, the present invention provides a method for detecting an extracellular vesicle, the method including: a step of labeling an extracellular vesicle in a sample using a first metal labeling reagent for labeling a nucleic acid and a second metal labeling reagent for labeling an extracellular vesicle surface antigen; and a step of identifying the first and second metal labeling reagents by mass spectrometry.

公开(公告)号：US20240027455A1

公开(公告)日：2024-01-25

申请号：US18265547

申请日：2021-12-06

Applicant: NATIONAL CANCER CENTER ,  SHIMADZU CORPORATION ,  DAI NIPPON TORYO CO., LTD.

Inventor： Kenji TAMURA ,  Kazuki SUDO ,  Takahisa MATSUDA ,  Taiki YAMAJI ,  Koji OKAMOTO ,  Makoto WATANABE ,  Hirotaka FUJIMOTO ,  Taka-Aki SATO ,  Yuta MIYAZAWA

IPC: G01N33/574 ,  G01N33/543 ,  C07K16/18

CPC classification number: G01N33/57415 ,  G01N33/57488 ,  G01N33/54387 ,  C07K16/18 ,  G01N2333/78 ,  G01N2333/70596

Abstract: Provided is a method of assisting breast cancer diagnosis, comprising: a measuring step for measuring an amount of laminin 5 or laminin β3 in a specimen; and an information-providing step for providing information for breast cancer diagnosis based on the amount of laminin 5 or laminin β3 thus measured. Provided is a test kit for breast cancer comprising an anti-laminin 5 antibody or an anti-laminin β3 antibody.

公开(公告)号：US20220146526A1

公开(公告)日：2022-05-12

申请号：US17437720

申请日：2020-01-27

Applicant: SHIMADZU CORPORATION

Inventor： Yumi UNNO ,  Shuichi KAWANA ,  Yutaka AOKI ,  Junichi MASUDA ,  Hirotaka FUJIMOTO ,  Tsuyoshi NAKANISHI

IPC: G01N33/68 ,  G01N33/70 ,  G01N33/92 ,  G01N30/72

Abstract: A method for evaluating a sample includes obtaining a blood plasma sample prepared from human blood, conducting detection of a predetermined molecule in the blood plasma sample, and evaluating the quality of the blood plasma sample based on the intensity of the molecule acquired by the detection.