公开(公告)号：US20220246231A1

公开(公告)日：2022-08-04

申请号：US17623777

申请日：2019-07-09

Applicant: SHIMADZU CORPORATION ,  KYOTO UNIVERSITY

Inventor： Makoto WATANABE ,  Taka-Aki SATO ,  Junya TOGUCHIDA

IPC: G16B5/00 ,  C12N5/077 ,  G16B25/10 ,  G16B40/20

Abstract: A plurality of metabolites contained in culture supernatants of a plurality of iPS cell clones whose differentiation efficiency into chondrocytes or neural crest cells is known is quantified, and a relationship between the measured values of the plurality of metabolites obtained by the quantification and the differentiation efficiencies is subjected to a multivariate analysis, and a model for predicting a differentiation efficiency of iPS cells is constructed. Furthermore, the plurality of metabolites contained in a culture supernatant of a test cell group including a single type of iPS cell clones is quantified, and the values, obtained by the quantification, are applied to the model, thereby predicting a differentiation efficiency of the test cell group into chondrocytes or neural crest cells. This makes it possible to predict the differentiation efficiency of iPS cells into chondrocytes or neural crest cells in a short time.

公开(公告)号：US20250130229A1

公开(公告)日：2025-04-24

申请号：US18847590

申请日：2023-03-17

Applicant: SHIMADZU CORPORATION

Inventor： Makoto WATANABE ,  Taka-Aki SATO ,  Yuta MIYAZAWA

IPC: G01N33/553 ,  G01N27/68

Abstract: Provided is a method of analyzing a biomolecule present at a vesicle, the method comprising: bringing a vesicle into contact with a capturing molecule solidified on a support; bringing a first detection molecule labeled with a first metal particle into contact with the vesicle; bringing a second detection molecule labeled with a second metal particle into contact with the vesicle; and quantifying the first metal particle and the second metal particle bonded to the vesicle by inductively coupled plasma (ICP) mass spectrometry or ICP emission spectroscopy.

公开(公告)号：US20240027455A1

公开(公告)日：2024-01-25

申请号：US18265547

申请日：2021-12-06

Applicant: NATIONAL CANCER CENTER ,  SHIMADZU CORPORATION ,  DAI NIPPON TORYO CO., LTD.

Inventor： Kenji TAMURA ,  Kazuki SUDO ,  Takahisa MATSUDA ,  Taiki YAMAJI ,  Koji OKAMOTO ,  Makoto WATANABE ,  Hirotaka FUJIMOTO ,  Taka-Aki SATO ,  Yuta MIYAZAWA

IPC: G01N33/574 ,  G01N33/543 ,  C07K16/18

CPC classification number: G01N33/57415 ,  G01N33/57488 ,  G01N33/54387 ,  C07K16/18 ,  G01N2333/78 ,  G01N2333/70596

Abstract: Provided is a method of assisting breast cancer diagnosis, comprising: a measuring step for measuring an amount of laminin 5 or laminin β3 in a specimen; and an information-providing step for providing information for breast cancer diagnosis based on the amount of laminin 5 or laminin β3 thus measured. Provided is a test kit for breast cancer comprising an anti-laminin 5 antibody or an anti-laminin β3 antibody.

公开(公告)号：US20240060980A1

公开(公告)日：2024-02-22

申请号：US18265816

申请日：2021-12-07

Applicant: DAI NIPPON TORYO CO., LTD. ,  SHIMADZU CORPORATION

Inventor： Yuta MIYAZAWA ,  Hirotaka FUJIMOTO ,  Makoto WATANABE ,  Taka-Aki SATO

IPC: G01N33/574 ,  G01N33/543

CPC classification number: G01N33/57415 ,  G01N33/57488 ,  G01N33/54387 ,  G01N2470/04

Abstract: An object of the present invention is to provide a method for detecting particulate substances with a high degree of sensitivity, while suppressing the generation of non-specific signals. According to the present invention, the generation of non-specific signals in the background can be suppressed by blocking a membrane of a test strip which is used for immunochromatography with a blocking composition including a polymer-based blocking agent having, in its side chain, a substituent represented by the following formula:




(wherein R1, R2, and R3 are each independently a hydrogen atom, a C1-6 alkyl group, or a C1-6 hydroxyalkyl group; and n is an integer of 1 to 4), and having a weight average molecular weight (Mw) of 1×103 to 1×107.

公开(公告)号：US20220317128A1

公开(公告)日：2022-10-06

申请号：US17640032

申请日：2020-09-07

Applicant: DAI NIPPON TORYO CO., LTD. ,  SHIMADZU CORPORATION

Inventor： Yuta MIYAZAWA ,  Daigou MIZOGUCHI ,  Hirotaka FUJIMOTO ,  Makoto WATANABE ,  Taka-Aki SATO

IPC: G01N33/574 ,  G01N33/543 ,  G01N21/76 ,  G01N21/64

Abstract: The present invention relates to a method for detecting a particulate substance by immunochromatography, the particulate substance including, on its surface, a plurality of substances to be bound containing a first substance to be bound and a second substance to be bound which may be the same or different with respect to each other, wherein the method includes the steps of: (1) contacting on a membrane a sample containing the particulate substance with a first specific binding substance for the first substance to be bound to capture the particulate substance with the first specific binding substance; (2) contacting the captured particulate substance with a second specific binding substance for the second substance to be bound to label the particulate substance; and (3) detecting the labeled particulate substance, wherein the first specific binding substance is immobilized on the membrane, and the second specific binding substance is bound to a metal nanoparticle.

公开(公告)号：US20220145376A1

公开(公告)日：2022-05-12

申请号：US17611603

申请日：2020-05-13

Applicant: SHIMADZU CORPORATION

Inventor： Makoto WATANABE ,  Hirotaka FUJIMOTO ,  Taka-Aki SATO

IPC: C12Q1/6818 ,  G01N27/62

Abstract: The present invention provides a method for quantifying a plurality of surface antigens simultaneously, thereby identifying an extracellular vesicle such as an exosome, and detecting and quantifying any surface antigen. Specifically, the present invention provides a method for detecting an extracellular vesicle, the method including: a step of labeling an extracellular vesicle in a sample using a first metal labeling reagent for labeling a nucleic acid and a second metal labeling reagent for labeling an extracellular vesicle surface antigen; and a step of identifying the first and second metal labeling reagents by mass spectrometry.