公开(公告)号：US20160024464A1

公开(公告)日：2016-01-28

申请号：US14840653

申请日：2015-08-31

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan JOHANSSON ,  Anna RISING ,  My HEDHAMMAR ,  Ulrika JOHANSSON ,  Mona WIDHE

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  A61L27/227 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/56 ,  C07K14/005 ,  C07K14/43518 ,  C12N5/0606 ,  C12N5/0623 ,  C12N5/0629 ,  C12N5/0647 ,  C12N5/0656 ,  C12N5/067 ,  C12N5/0676 ,  C12N5/0691 ,  C12N2500/82 ,  C12N2533/50 ,  C12N2539/00 ,  D01F4/02

Abstract: A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.

Abstract translation: 提供了用于培养真核细胞的方法和组合，以及用于制备真核细胞的方法。 所述方法包括提供待培养的真核细胞样品，将所述样品施用于细胞支架材料; 以及在适于细胞培养的条件下保持所述细胞支架材料具有施加于其上的细胞。 该组合包含真核细胞和细胞支架材料。 细胞支架材料包括蜘蛛丝蛋白的聚合物。

公开(公告)号：US20190144819A1

公开(公告)日：2019-05-16

申请号：US16077179

申请日：2017-02-10

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Mona WIDHE ,  Ulrika JOHANSSON

IPC: C12N5/00 ,  C07K14/435

Abstract: A cell scaffold material is manufactured by providing an aqueous solution of a silk protein capable of assembling into a water-insoluble macrostructure. The silk protein is mixed with eukaryotic cells, and the silk protein is assembled into a water-insoluble macrostructure in the presence of the cells, thereby forming a scaffold material for cultivating the cells. The cells can be grown integrated with the scaffold material under conditions suitable for cell culture.