公开(公告)号：US20170283474A1

公开(公告)日：2017-10-05

申请号：US15490328

申请日：2017-04-18

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan JOHANSSON ,  Anna RISING ,  My HEDHAMMAR ,  Kerstin NORDLING

IPC: C07K14/435 ,  C12N15/62

CPC classification number: C07K14/43518 ,  C12N15/62

Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

公开(公告)号：US20210069939A1

公开(公告)日：2021-03-11

申请号：US16644772

申请日：2018-09-26

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Linnea Gustafsson ,  Ronnie Jansson ,  Wouter van der Wijngaart

IPC: B29C39/02 ,  C07K14/435 ,  B29C33/42 ,  B29C39/10 ,  B29C41/12 ,  C09D127/12

Abstract: A method for manufacturing shaped polymers of surface-active macromolecules, in particular silk, is provided. The method is comprising the steps of: • a) depositing an aqueous solution of the surface-active macromolecules on a surface, wherein the aqueous solution of the surface-active macromolecules is deposited in the form of a droplet, and wherein the surface is a hydrophobic micropatterned surface adapted to prevent the aqueous solution from penetrating into the pattern and to receive the droplet of the aqueous solution of the surface-active macromolecules and retain its droplet state; and • b) forming shaped polymers of the surface-active macromolecules on the surface.

公开(公告)号：US20150291674A1

公开(公告)日：2015-10-15

申请号：US14753101

申请日：2015-06-29

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Jan JOHANSSON ,  Anna RISING ,  Per Åke NYGREN

IPC: C07K14/435 ,  C07K14/76 ,  B01D15/20 ,  C07K14/31 ,  C12N5/00 ,  C07K16/06 ,  C07D495/04

CPC classification number: C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

Abstract: A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

Abstract translation: 提供能够与有机靶相互选择性的蛋白质结构。 蛋白质结构是包含能够与有机靶选择性相互作用的重组融合蛋白作为重复结构单元的聚合物。 融合蛋白包含部分B，REP和CT，以及任选的NT。 B是超过30个氨基酸残基的非宿主部分，其提供与有机靶的选择性相互作用的能力。 REP是70至300个氨基酸残基的部分，并衍生自蜘蛛丝蛋白的重复片段。 CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段。 NT是100至160个氨基酸残基的任选部分，并衍生自蜘蛛丝蛋白的N-末端片段。 其融合蛋白质和蛋白质结构可用作亲和介质和细胞支架材料。

公开(公告)号：US20190144819A1

公开(公告)日：2019-05-16

申请号：US16077179

申请日：2017-02-10

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Mona WIDHE ,  Ulrika JOHANSSON

IPC: C12N5/00 ,  C07K14/435

Abstract: A cell scaffold material is manufactured by providing an aqueous solution of a silk protein capable of assembling into a water-insoluble macrostructure. The silk protein is mixed with eukaryotic cells, and the silk protein is assembled into a water-insoluble macrostructure in the presence of the cells, thereby forming a scaffold material for cultivating the cells. The cells can be grown integrated with the scaffold material under conditions suitable for cell culture.

公开(公告)号：US20200308727A1

公开(公告)日：2020-10-01

申请号：US16651232

申请日：2018-10-09

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Mathias KVICK

IPC: D01D5/40 ,  D01F4/00

Abstract: A method for producing a protein polymer fiber, the method comprising providing a liquid protein solution in a container for liquid, and repeatedly moving the liquid surface in the container back and forth between a first and a second position. Said movement of the liquid surface is such that the protein polymer solution is allowed to form a film in the interface between the liquid surface of the liquid protein solution and a surrounding fluid. The movement of the liquid surface being performed by respectively raising and lowering the liquid surface relative to the container or by moving an object extending through the liquid surface of the liquid protein solution. Also, a device for performing said method.

公开(公告)号：US20190151505A1

公开(公告)日：2019-05-23

申请号：US16301819

申请日：2017-05-16

Applicant: SPIBER TECHNOLOGIES AB

Inventor： My HEDHAMMAR ,  Linnea NILEBÄCK

IPC: A61L27/36 ,  A61L27/34 ,  A61L27/22 ,  A61L27/12 ,  C12N5/00

Abstract: A method for coating a solid surface with a recombinant spider silk protein capable of forming polymeric, solid structures is provided. The method is comprising the following steps: exposing the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming a surface layer of the recombinant spider silk protein adsorbed on the solid surface without formation of covalent bonds between the recombinant spider silk protein and the solid surface; and further exposing the surface layer of the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming an assembled silk structure layer of the recombinant spider silk protein on the surface layer; wherein the method does not include drying-in of spider silk protein.

公开(公告)号：US20160024464A1

公开(公告)日：2016-01-28

申请号：US14840653

申请日：2015-08-31

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan JOHANSSON ,  Anna RISING ,  My HEDHAMMAR ,  Ulrika JOHANSSON ,  Mona WIDHE

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  A61L27/227 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/56 ,  C07K14/005 ,  C07K14/43518 ,  C12N5/0606 ,  C12N5/0623 ,  C12N5/0629 ,  C12N5/0647 ,  C12N5/0656 ,  C12N5/067 ,  C12N5/0676 ,  C12N5/0691 ,  C12N2500/82 ,  C12N2533/50 ,  C12N2539/00 ,  D01F4/02

Abstract: A method and a combination for the cultivation of eukaryotic cells are provided, as well as a method for preparation of eukaryotic cells. The methods comprise providing a sample of eukaryotic cells to be cultured, applying said sample to a cell scaffold material; and maintaining said cell scaffold material having cells applied thereto under conditions suitable for cell culture. The combination comprises eukaryotic cells and a cell scaffold material. The cell scaffold material comprises a polymer of a spider silk protein.

Abstract translation: 提供了用于培养真核细胞的方法和组合，以及用于制备真核细胞的方法。 所述方法包括提供待培养的真核细胞样品，将所述样品施用于细胞支架材料; 以及在适于细胞培养的条件下保持所述细胞支架材料具有施加于其上的细胞。 该组合包含真核细胞和细胞支架材料。 细胞支架材料包括蜘蛛丝蛋白的聚合物。

公开(公告)号：US20140079674A1

公开(公告)日：2014-03-20

申请号：US14086244

申请日：2013-11-21

Applicant: Spiber Technologies AB

Inventor： Jan JOHANSSON ,  Goran HJALM ,  Margareta STARK ,  Anna RISING ,  Stefan GRIP ,  Wilhelm ENGSTROM ,  My HEDHAMMAR

IPC: C07K14/435 ,  A61K47/42 ,  A61K35/12

CPC classification number: C07K14/43518 ,  A61K35/12 ,  A61K47/42 ,  D01F4/00

Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式REP-CT定义。 REP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。