公开(公告)号：US20240426816A1

公开(公告)日：2024-12-26

申请号：US18750208

申请日：2024-06-21

Applicant: StemCell Technologies Canada Inc.

Inventor： Samuel Jon Clarke ,  Adil Kassam

IPC: G01N33/533 ,  G01N33/532 ,  G01N33/569 ,  G01N33/58

Abstract: This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, extracellular vesicles, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.

公开(公告)号：US11725042B2

公开(公告)日：2023-08-15

申请号：US16912882

申请日：2020-06-26

Applicant: STEMCELL Technologies Canada Inc.

Inventor： Adil Kassam ,  Eric Jervis

IPC: C07K14/705 ,  C07K16/28 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/541 ,  G01N33/543 ,  G01N33/566 ,  G01N33/577 ,  A61K47/69 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  G01N33/537

CPC classification number: C07K14/70596 ,  A61K47/6913 ,  C07K16/2896 ,  C12M21/00 ,  C12M25/14 ,  C12M41/36 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/537 ,  G01N33/541 ,  G01N33/54346 ,  G01N33/566 ,  G01N33/577 ,  C07K2319/03 ,  G01N2333/705 ,  G01N2333/70596

Abstract: A method of recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants is described. In one embodiment, the method comprises: a) removing contaminants from the sample, wherein the contaminants are relatively larger or more dense than the extracellular vesicles; b) contacting the sample of step a) with a plurality of binding compositions, each binding composition having first and/or second moieties capable of binding a recognition motif of the target entities under conditions to allow complexing of the extracellular vesicles with the plurality of binding compositions to form a complexed population of extracellular vesicles, the complexed population of extracellular vesicles having an increased volume and/or higher density in comparison to the extracellular vesicles in individual form; and c) recovering the complexed population of extracellular vesicles.

公开(公告)号：US20230374106A1

公开(公告)日：2023-11-23

申请号：US18352717

申请日：2023-07-14

Applicant: STEMCELL Technologies Canada Inc.

Inventor： Adil Kassam ,  Eric Jervis

IPC: C07K14/705 ,  G01N33/577 ,  C07K16/28 ,  G01N33/543 ,  G01N33/48 ,  G01N33/53 ,  G01N33/566 ,  G01N33/541 ,  C12M1/00 ,  C12M1/12 ,  G01N33/50 ,  C12M1/34 ,  G01N33/537 ,  A61K47/69

CPC classification number: C07K14/70596 ,  G01N33/577 ,  C07K16/2896 ,  G01N33/54346 ,  G01N33/48 ,  G01N33/53 ,  G01N33/566 ,  G01N33/541 ,  C12M21/00 ,  C12M25/14 ,  G01N33/50 ,  C12M41/36 ,  G01N33/537 ,  A61K47/6913 ,  G01N2333/70596 ,  G01N2333/705 ,  C07K2319/03

Abstract: A device for recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants is described.

公开(公告)号：US20190178879A1

公开(公告)日：2019-06-13

申请号：US16227436

申请日：2018-12-20

Applicant: StemCell Technologies Canada Inc.

Inventor： Samuel Jon Clarke ,  Adil Kassam

IPC: G01N33/533 ,  G01N33/569

Abstract: This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, extracellular vesicles, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.