公开(公告)号：US20250163359A1

公开(公告)日：2025-05-22

申请号：US19032526

申请日：2025-01-21

Applicant: STEMCELL TECHNOLOGIES CANADA INC.

Inventor： Emanuel Nazareth ,  Eric Jervis ,  Martin O'Keane ,  Carrick Faris

IPC: C12M1/36 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34 ,  C12M3/00 ,  C12N5/074

Abstract: Systems and methods for the automated culture of cells and apparatus and methods for dissociating one or more colonies in a culture of cells are described. The automated systems include at least an imaging module, a pipette module, a handling module, and optionally a stage module. Coordinated operation of the foregoing modules is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at a first time point and at one or more subsequent time points. The apparatus includes a mounting surface, having one or more impact bumpers, connected to a rotatable drive, a platform rotatable dependently or independently of the drive, and one or more impact brackets connected to the platform and collidable with the one or more impact bumpers to transmit a dissociative force to a culture of cells in a cell culture vessel upon the platform.

公开(公告)号：US20230374106A1

公开(公告)日：2023-11-23

申请号：US18352717

申请日：2023-07-14

Applicant: STEMCELL Technologies Canada Inc.

Inventor： Adil Kassam ,  Eric Jervis

IPC: C07K14/705 ,  G01N33/577 ,  C07K16/28 ,  G01N33/543 ,  G01N33/48 ,  G01N33/53 ,  G01N33/566 ,  G01N33/541 ,  C12M1/00 ,  C12M1/12 ,  G01N33/50 ,  C12M1/34 ,  G01N33/537 ,  A61K47/69

CPC classification number: C07K14/70596 ,  G01N33/577 ,  C07K16/2896 ,  G01N33/54346 ,  G01N33/48 ,  G01N33/53 ,  G01N33/566 ,  G01N33/541 ,  C12M21/00 ,  C12M25/14 ,  G01N33/50 ,  C12M41/36 ,  G01N33/537 ,  A61K47/6913 ,  G01N2333/70596 ,  G01N2333/705 ,  C07K2319/03

Abstract: A device for recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants is described.

公开(公告)号：US20210317399A1

公开(公告)日：2021-10-14

申请号：US17253838

申请日：2019-06-19

Applicant: STEMCELL TECHNOLOGIES CANADA INC.

Inventor： Emanuel Nazareth ,  Eric Jervis ,  Martin O'Keane ,  Carrick Faris

IPC: C12M1/36 ,  C12M1/26 ,  C12M1/34 ,  C12M1/00 ,  C12N5/074

Abstract: Systems and methods for the automated culture of cells and apparatus and methods for dissociating one or more colonies in a culture of cells are described. The automated systems include at least an imaging module, a pipette module, a handling module, and optionally a stage module. Coordinated operation of the foregoing modules is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at a first time point and at one or more subsequent time points. The apparatus includes a mounting surface, having one or more impact bumpers, connected to a rotatable drive, a platform rotatable dependently or independently of the drive, and one or more impact brackets connected to the platform and collidable with the one or more impact bumpers to transmit a dissociative force to a culture of cells in a cell culture vessel upon the platform.

公开(公告)号：US20230017094A1

公开(公告)日：2023-01-19

申请号：US17779830

申请日：2020-11-26

Applicant: StemCell Technologies Canada Inc.

Inventor： Emanuel Nazareth ,  Charis Segeritz-Walko ,  Philipp Kramer ,  Eric Jervis ,  Colleen Umali ,  Juan Hou ,  Tyler Brown ,  Nina Quiskamp

IPC: C12N5/071 ,  C12M1/32 ,  C12N5/00

Abstract: Described are devices for tethering biological materials, which in applicable embodiments support the growth and differentiation thereof. In a specific embodiment, the biological materials are cells and the cells grow/differentiate into tethered three-dimensional aggregates. The devices disclosed herein may be used in various methods/assays relating to tethered biological materials, such as to tethered three-dimensional aggregates of cells.

公开(公告)号：US12258552B2

公开(公告)日：2025-03-25

申请号：US17620511

申请日：2019-12-18

Applicant: StemCell Technologies Canada Inc.

Inventor： Emanuel Nazareth ,  Eric Jervis ,  Martin O'Keane ,  Tia Sojonky ,  Mark Romanish

IPC: C12M1/36 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34 ,  C12M3/00 ,  C12N5/074

Abstract: Apparatus, systems and methods for the adaptive passage of a culture of cells and apparatus and methods for dissociating cell colonies are described. The systems may include an imaging module, a pipette module, a handling module, and/or a stage module. Coordinated operation of the modules, optionally in an automated manner, is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at more than one time point. A first apparatus for adaptive passage of a culture cells includes an imaging module and at least one processor, which apparatus may be included in the systems or used in the methods. A second apparatus for dissociating cell colonies, may also be included in the systems or used in the methods, includes impact bumper(s) collidable with impact bracket(s) to transmit a dissociative force to a culture of cells.

公开(公告)号：US11725042B2

公开(公告)日：2023-08-15

申请号：US16912882

申请日：2020-06-26

Applicant: STEMCELL Technologies Canada Inc.

Inventor： Adil Kassam ,  Eric Jervis

IPC: C07K14/705 ,  C07K16/28 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/541 ,  G01N33/543 ,  G01N33/566 ,  G01N33/577 ,  A61K47/69 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  G01N33/537

CPC classification number: C07K14/70596 ,  A61K47/6913 ,  C07K16/2896 ,  C12M21/00 ,  C12M25/14 ,  C12M41/36 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/537 ,  G01N33/541 ,  G01N33/54346 ,  G01N33/566 ,  G01N33/577 ,  C07K2319/03 ,  G01N2333/705 ,  G01N2333/70596

Abstract: A method of recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants is described. In one embodiment, the method comprises: a) removing contaminants from the sample, wherein the contaminants are relatively larger or more dense than the extracellular vesicles; b) contacting the sample of step a) with a plurality of binding compositions, each binding composition having first and/or second moieties capable of binding a recognition motif of the target entities under conditions to allow complexing of the extracellular vesicles with the plurality of binding compositions to form a complexed population of extracellular vesicles, the complexed population of extracellular vesicles having an increased volume and/or higher density in comparison to the extracellular vesicles in individual form; and c) recovering the complexed population of extracellular vesicles.