公开(公告)号：US20180289015A1

公开(公告)日：2018-10-11

申请号：US15765941

申请日：2016-09-27

Applicant: SYNGENTA PARTICIPATIONS AG ,  DEVGEN NV

Inventor： Pascale Feldmann ,  Jeffrey David Fowler ,  Wendy Maddelein ,  Nina Cromheecke ,  Isabelle Maillet

IPC: A01N63/02 ,  C12N15/10 ,  C12N15/113 ,  C12N15/11

Abstract: The present invention provides a method of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell lysate, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the lysate a compound having the function of a protein- or amine-cross linking agent. The invention also comprises compositions comprising the lysate comprising dsRNA, and protein cross linking agents, as well as the use of said agents in the method.

公开(公告)号：US11286476B2

公开(公告)日：2022-03-29

申请号：US16339866

申请日：2017-09-28

Applicant: SYNGENTA PARTICIPATIONS AG ,  Devgen NV

Inventor： Pascale Feldmann ,  Jeffrey David Fowler ,  Nema Devi Jhurry ,  Isabelle Maillet ,  Marta Omedes Pujol

IPC: C12N15/11 ,  C12N15/10 ,  A01N63/60

Abstract: Methods of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the cell composition a compound having the function of a protein—or amine—cross linking agent and/or an acid, and compositions comprising the cells comprising dsRNA, and protein cross linking agents and/or acids, as well as the use of the cross linking agents and/or acids in the method.

公开(公告)号：US11945843B2

公开(公告)日：2024-04-02

申请号：US16321904

申请日：2017-08-01

Applicant: Syngenta Participations AG

Inventor： Kevin L. Donohue ,  Yann Naudet ,  Pascale Feldmann ,  Lies Degrave ,  Isabelle Maillet

IPC: C07K14/42 ,  C07K14/325 ,  C07K16/18 ,  C12N9/42 ,  C12N15/82

CPC classification number: C07K14/42 ,  C07K14/325 ,  C07K16/18 ,  C12N9/2442 ,  C12N15/8218 ,  C12N15/8286 ,  C12Y302/01014 ,  Y02A40/146

Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

公开(公告)号：US10898507B2

公开(公告)日：2021-01-26

申请号：US15746449

申请日：2017-08-01

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Kevin V. Donohue ,  Yann Naudet ,  Pascale Feldmann ,  Lies Degrave ,  Isabelle Maillet

IPC: C12N15/11 ,  A61K31/713 ,  A61K45/06 ,  C12N15/113 ,  C12N15/82 ,  A01N63/10 ,  A01C1/06 ,  C12N15/87

Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

公开(公告)号：US20180200281A1

公开(公告)日：2018-07-19

申请号：US15746449

申请日：2017-08-01

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Kevin V. Donohue ,  Yann Naudet ,  Pascale Feldmann ,  Lies Degrave ,  Isabelle Maillet

IPC: A61K31/713 ,  A01C1/06 ,  A01N63/02 ,  A61K45/06 ,  C12N15/113 ,  C12N15/82 ,  C12N15/87

CPC classification number: A61K31/713 ,  A01C1/06 ,  A01N63/02 ,  A61K45/06 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8286 ,  C12N15/87 ,  C12N2310/14 ,  Y02A40/162

Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

公开(公告)号：US12016339B2

公开(公告)日：2024-06-25

申请号：US16756232

申请日：2018-11-06

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Yann Naudet ,  Myriam Beghyn ,  Lien De Schrijver ,  Annelies Philips ,  Isabelle Maillet

IPC: C12N15/113 ,  A01N63/60

CPC classification number: A01N63/60 ,  C12N15/113 ,  C12N2310/14

Abstract: Disclosed are double stranded RNA (dsRNA) molecules that are toxic to insect pests. In particular, interfering RNA molecules capable of modulating expression of a pest insect target gene and that are toxic to the insect pest are provided. Further, methods of making and using the interfering RNA, for example as the active ingredient in an insecticidal composition or in a transgenic plant, to confer protection from insect damage are disclosed.

公开(公告)号：US20210186029A1

公开(公告)日：2021-06-24

申请号：US16756232

申请日：2018-11-06

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Yann Naudet ,  Myriam Beghyn ,  Lien De Schrijver ,  Annelies Philips ,  Isabelle Maillet

IPC: A01N63/60 ,  C12N15/113 ,  C12Q1/6888

Abstract: Disclosed are double stranded RNA (dsRNA) molecules that are toxic to insect pests. In particular, interfering RNA molecules capable of modulating expression of a pest insect target gene and that are toxic to the insect pest are provided. Further, methods of making and using the interfering RNA, for example as the active ingredient in an insecticidal composition or in a transgenic plant, to confer protection from insect damage are disclosed.

公开(公告)号：US20200239891A1

公开(公告)日：2020-07-30

申请号：US16756298

申请日：2018-11-06

Applicant: DEVGEN NV

Inventor： Yann Naudet ,  Myriam Beghyn ,  Lien De Schrijver ,  Isabelle Maillet ,  Annelies Philips

IPC: C12N15/113 ,  C07H21/02 ,  C12N15/63

Abstract: Disclosed are double stranded RNA (dsRNA) molecules that are toxic to insect pests. In particular, interfering RNA molecules capable of modulating expression of a pest insect target gene and that are toxic to the insect pest are provided. Further, methods of making and using the interfering RNA, for example as the active ingredient in an insecticidal composition or in a transgenic plant, to confer protection from insect damage are disclosed.

公开(公告)号：US11198868B2

公开(公告)日：2021-12-14

申请号：US16756298

申请日：2018-11-06

Applicant: DEVGEN NV

Inventor： Yann Naudet ,  Myriam Beghyn ,  Lien De Schrijver ,  Isabelle Maillet ,  Annelies Philips

IPC: C12N15/113 ,  C07H21/02 ,  C12N15/63

Abstract: Disclosed are double stranded RNA (dsRNA) molecules that are toxic to insect pests. In particular, interfering RNA molecules capable of modulating expression of a pest insect target gene and that are toxic to the insect pest are provided. Further, methods of making and using the interfering RNA, for example as the active ingredient in an insecticidal composition or in a transgenic plant, to confer protection from insect damage are disclosed.