公开(公告)号：US20180289015A1

公开(公告)日：2018-10-11

申请号：US15765941

申请日：2016-09-27

Applicant: SYNGENTA PARTICIPATIONS AG ,  DEVGEN NV

Inventor： Pascale Feldmann ,  Jeffrey David Fowler ,  Wendy Maddelein ,  Nina Cromheecke ,  Isabelle Maillet

IPC: A01N63/02 ,  C12N15/10 ,  C12N15/113 ,  C12N15/11

Abstract: The present invention provides a method of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell lysate, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the lysate a compound having the function of a protein- or amine-cross linking agent. The invention also comprises compositions comprising the lysate comprising dsRNA, and protein cross linking agents, as well as the use of said agents in the method.

公开(公告)号：US11286476B2

公开(公告)日：2022-03-29

申请号：US16339866

申请日：2017-09-28

Applicant: SYNGENTA PARTICIPATIONS AG ,  Devgen NV

Inventor： Pascale Feldmann ,  Jeffrey David Fowler ,  Nema Devi Jhurry ,  Isabelle Maillet ,  Marta Omedes Pujol

IPC: C12N15/11 ,  C12N15/10 ,  A01N63/60

Abstract: Methods of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the cell composition a compound having the function of a protein—or amine—cross linking agent and/or an acid, and compositions comprising the cells comprising dsRNA, and protein cross linking agents and/or acids, as well as the use of the cross linking agents and/or acids in the method.

公开(公告)号：US11945843B2

公开(公告)日：2024-04-02

申请号：US16321904

申请日：2017-08-01

Applicant: Syngenta Participations AG

Inventor： Kevin L. Donohue ,  Yann Naudet ,  Pascale Feldmann ,  Lies Degrave ,  Isabelle Maillet

IPC: C07K14/42 ,  C07K14/325 ,  C07K16/18 ,  C12N9/42 ,  C12N15/82

CPC classification number: C07K14/42 ,  C07K14/325 ,  C07K16/18 ,  C12N9/2442 ,  C12N15/8218 ,  C12N15/8286 ,  C12Y302/01014 ,  Y02A40/146

Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

公开(公告)号：US10898507B2

公开(公告)日：2021-01-26

申请号：US15746449

申请日：2017-08-01

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Kevin V. Donohue ,  Yann Naudet ,  Pascale Feldmann ,  Lies Degrave ,  Isabelle Maillet

IPC: C12N15/11 ,  A61K31/713 ,  A61K45/06 ,  C12N15/113 ,  C12N15/82 ,  A01N63/10 ,  A01C1/06 ,  C12N15/87

Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

公开(公告)号：US20180200281A1

公开(公告)日：2018-07-19

申请号：US15746449

申请日：2017-08-01

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Kevin V. Donohue ,  Yann Naudet ,  Pascale Feldmann ,  Lies Degrave ,  Isabelle Maillet

IPC: A61K31/713 ,  A01C1/06 ,  A01N63/02 ,  A61K45/06 ,  C12N15/113 ,  C12N15/82 ,  C12N15/87

CPC classification number: A61K31/713 ,  A01C1/06 ,  A01N63/02 ,  A61K45/06 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8286 ,  C12N15/87 ,  C12N2310/14 ,  Y02A40/162

Abstract: Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

公开(公告)号：US09957523B2

公开(公告)日：2018-05-01

申请号：US14324351

申请日：2014-07-07

Applicant: DEVGEN NV

Inventor： Romaan Raemaekers ,  Pascale Feldmann ,  Irene Nooren ,  Geert Plaetinck ,  Frederic Pecqueur ,  Els Van Bleu ,  Thierry Bogaert

IPC: C12N15/82 ,  A01N57/10 ,  C12N15/113

CPC classification number: C12N15/8286 ,  A01N57/10 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8285 ,  C12N2310/14 ,  Y02A40/162 ,  Y02A40/164

Abstract: The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for making transgenic plants that express the double stranded RNA molecules, as well as pesticidal agents and commodity products produced by the inventive plants.

公开(公告)号：US10329565B2

公开(公告)日：2019-06-25

申请号：US15838522

申请日：2017-12-12

Applicant: DEVGEN NV

Inventor： Myriam Beghyn ,  Thierry Andre Olivier Eddy Bogaert ,  Pascale Feldmann ,  Romaan Raemaekers

IPC: C12N15/82 ,  A01N57/16 ,  A01N63/00 ,  C07K14/435 ,  C12N15/113

Abstract: The present invention relates to genetic control of infestation by insect pest species, particularly prevention and/or control of pest infestation of plants, using interfering ribonucleic acid (RNA) molecules. Compositions and combinations containing the interfering RNA molecules of the invention for use in topical applications, for example in the form of insecticides.

公开(公告)号：US09914925B2

公开(公告)日：2018-03-13

申请号：US14930753

申请日：2015-11-03

Applicant: DEVGEN NV

Inventor： Myriam Beghyn ,  Thierry Andre Olivier Eddy Bogaert ,  Pascale Feldmann ,  Romaan Raemaekers

IPC: C12N15/113 ,  A01N63/00 ,  C12N15/82 ,  C07K14/435 ,  A01N57/16

CPC classification number: C12N15/113 ,  A01N57/16 ,  A01N63/00 ,  C07K14/43563 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  Y02A40/162

Abstract: The present invention relates to genetic control of infestation by insect pest species, particularly prevention and/or control of pest infestation of plants, using interfering ribonucleic acid (RNA) molecules. Compositions and combinations containing the interfering RNA molecules of the invention for use in topical applications, for example in the form of insecticides.

公开(公告)号：US20190256851A1

公开(公告)日：2019-08-22

申请号：US16405156

申请日：2019-05-07

Applicant: DEVGEN NV

Inventor： Myriam Beghyn ,  Thierry Andre Olivier Eddy Bogaert ,  Pascale Feldmann ,  Romaan Raemaekers

IPC: C12N15/113 ,  A01N57/16 ,  C12N15/82 ,  A01N63/00 ,  C07K14/435

Abstract: The present invention relates to genetic control of infestation by insect pest species, particularly prevention and/or control of pest infestation of plants, using interfering ribonucleic acid (RNA) molecules. Compositions and combinations containing the interfering RNA molecules of the invention for use in topical applications, for example in the form of insecticides.

公开(公告)号：US20180094265A1

公开(公告)日：2018-04-05

申请号：US15838522

申请日：2017-12-12

Applicant: DEVGEN NV

Inventor： Myriam Beghyn ,  Thierry Andre Olivier Eddy Bogaert ,  Pascale Feldmann ,  Romaan Raemaekers

IPC: C12N15/113 ,  A01N63/00 ,  C07K14/435 ,  C12N15/82 ,  A01N57/16

CPC classification number: C12N15/113 ,  A01N57/16 ,  A01N63/00 ,  C07K14/43563 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  Y02A40/162

Abstract: The present invention relates to genetic control of infestation by insect pest species, particularly prevention and/or control of pest infestation of plants, using interfering ribonucleic acid (RNA) molecules. Compositions and combinations containing the interfering RNA molecules of the invention for use in topical applications, for example in the form of insecticides.