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    Characterisation of gene function using double stranded RNA inhibition
    3.
    发明申请
    Characterisation of gene function using double stranded RNA inhibition 审中-公开

    公开(公告)号：US20040133943A1

    公开(公告)日：2004-07-08

    申请号：US10738886

    申请日：2003-12-17

    Applicant: Devgen NV

    Inventor： Geert Plaetinck ,  Christ Platteeuw ,  Katharine Mortier ,  Thierry Bogaert

    IPC: A01H001/00 ,  C12N015/82

    CPC classification number: C12N15/1079 ,  C12N15/1093 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/86 ,  C12N2795/10243 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12Q1/68 ,  Y02A40/164

    Abstract: There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of said cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of said cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to said promoter(s), b) introducing said library into one or more of said cells comprising said transcription factor, and c) identifying and isolating a particular phenotype of said cell comprising said library and identifying the DNA or cDNA fragment from said library responsible for conferring said phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of said DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from said cell, c) cloning said homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from said DNA homologue or fragment upon binding of an appropriate transcription factor to said promoter(s) and d) introducing said vector into said cell from step a) comprising said transcription factor.

    CONTROL OF INSECT PESTS USING RNA MOLECULES
    4.
    发明申请
    CONTROL OF INSECT PESTS USING RNA MOLECULES 有权

    公开(公告)号：US20220170018A1

    公开(公告)日：2022-06-02

    申请号：US17593594

    申请日：2021-03-13

    Applicant: DEVGEN NV

    Inventor： Lien DE SCHRIJVER ,  Kaat CAPPELLE ,  Lies DEGRAVE ,  Myriam BEGHYN ,  Yann NAUDET

    IPC: C12N15/113 ,  A01N63/60 ,  C12N15/82 ,  A01N63/50

    Abstract: Disclosed are double stranded RNA molecules that are toxic to flea beetles, particularly the flea beetle species Psylliodes chrysocephala, Phyllotreta nemorum, Phyllotreta striolata, Phyllotreta armoraciae, Phyllotreta atra and Phyllotreta cruciferae. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

    DOWN-REGULATING GENE EXPRESSION IN INSECT PLANTS
    6.
    发明申请
    DOWN-REGULATING GENE EXPRESSION IN INSECT PLANTS 有权

    公开(公告)号：US20210254093A1

    公开(公告)日：2021-08-19

    申请号：US17195247

    申请日：2021-03-08

    Applicant: Devgen NV

    Inventor： Thierry BOGAERT ,  Romaan RAEMAEKERS ,  Yann NAUDET

    IPC: C12N15/82 ,  C12N15/113

    Abstract: The present invention relates to the prevention and/or control of infestation by insect pest species. In particular, the invention relates to down-regulation of expression of target genes in insect pests using interfering ribonucleic acid (RNA) molecules. Also described are transgenic plants that (i) express or are capable of expressing interfering RNAs of the invention and (ii) are resistant to infestation by insect pest species. Compositions containing the interfering RNAs of the invention are also provided.

    CONTROL OF INSECT PESTS USING RNA MOLECULES
    7.
    发明申请
    CONTROL OF INSECT PESTS USING RNA MOLECULES 有权

    公开(公告)号：US20210123069A1

    公开(公告)日：2021-04-29

    申请号：US17051048

    申请日：2019-04-17

    Applicant: DEVGEN NV

    Inventor： Lien DE SCHRIJVER ,  Kaat CAPPELLE ,  Lies DEGRAVE ,  Yann NAUDET ,  Steffy DENORME

    IPC: C12N15/82 ,  A01N63/60

    Abstract: Disclosed are double stranded RNA molecules that are toxic to flea beetles, particularly the flea beetle species Psylliodes chrysocephala, Phyllotreta nemorum, Phyllotreta striolata, Phyllotreta armoraciae, and Phyllotreta cruciferae. In particular, interfering RNA molecules capable of interfering with pest target genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants or as the active ingredient in a composition, to confer protection from insect damage are disclosed.

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