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公开(公告)号：US20130210067A1
公开(公告)日：2013-08-15
申请号：US13825881
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
IPC分类号： G01N21/64
摘要： The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.
摘要翻译： 本发明提供了检测预定生物活性的方法。 该方法包括使用包含具有第一吸收光谱的第一指示剂和第二指示剂试剂的含水混合物。 第二指示剂通过预定的生物活性转化为具有第二发射光谱的第二生物衍生物。 第一吸收光谱包括在第二发射光谱中存在的波长的至少一部分中的可检测吸光度。 第一指示剂被含水液体接收并通过底物浓缩，便于检测第二生物衍生物。
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公开(公告)号：US08802392B2
公开(公告)日：2014-08-12
申请号：US13825881
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
IPC分类号： C12Q1/22
摘要： The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.
摘要翻译： 本发明提供了检测预定生物活性的方法。 该方法包括使用包含具有第一吸收光谱的第一指示剂和第二指示剂试剂的含水混合物。 第二指示剂通过预定的生物活性转化为具有第二发射光谱的第二生物衍生物。 第一吸收光谱包括在第二发射光谱中存在的波长的至少一部分中的可检测吸光度。 第一指示剂被含水液体接收并通过底物浓缩，便于检测第二生物衍生物。
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公开(公告)号：US20110182770A1
公开(公告)日：2011-07-28
申请号：US13063945
申请日：2009-10-12
申请人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
发明人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
IPC分类号： A61L2/18
摘要： A biological sterilization indicator, system, and methods of determining the effectiveness of a sterilization process. The biological sterilization indicator can include a locus of spores, a reservoir containing a liquid, and a sterilant path positioned to provide fluid communication between ambience and the locus of spores. The reservoir can have a closed state in which the reservoir is not in fluid communication with the locus of spores and an open state in which the reservoir is in fluid communication with the locus of spores. The biological sterilization indicator system can include the biological sterilization indicator and a detection device adapted to be coupled to the biological sterilization indicator. In some embodiments, the method can include assaying the spores for a detectable change in a characteristic, and detecting substantially all of the detectable change.
摘要翻译： 一种生物灭菌指标，系统和确定灭菌过程有效性的方法。 生物灭菌指示剂可以包括孢子的位点，含有液体的储存器和定位成在气氛和孢子的位置之间提供流体连通的消毒剂通道。 储存器可以具有闭合状态，其中储存器不与孢子的轨迹流体连通，并且储存器与孢子的轨迹流体连通的打开状态。 生物灭菌指示器系统可以包括生物灭菌指示器和适于联接到生物灭菌指示器的检测装置。 在一些实施方案中，该方法可以包括测定孢子以获得特征的可检测变化，并且检测基本上所有可检测的变化。
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公开(公告)号：US08969029B2
公开(公告)日：2015-03-03
申请号：US13063945
申请日：2009-10-12
申请人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
发明人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
摘要： A biological sterilization indicator, system, and methods of determining the effectiveness of a sterilization process. The biological sterilization indicator can include a locus of spores, a reservoir containing a liquid, and a sterilant path positioned to provide fluid communication between ambience and the locus of spores. The reservoir can have a closed state in which the reservoir is not in fluid communication with the locus of spores and an open state in which the reservoir is in fluid communication with the locus of spores. The biological sterilization indicator system can include the biological sterilization indicator and a detection device adapted to be coupled to the biological sterilization indicator. In some embodiments, the method can include assaying the spores for a detectable change in a characteristic, and detecting substantially all of the detectable change.
摘要翻译： 一种生物灭菌指标，系统和确定灭菌过程有效性的方法。 生物灭菌指示剂可以包括孢子的位点，含有液体的储存器和定位成在气氛和孢子的位置之间提供流体连通的消毒剂通道。 储存器可以具有闭合状态，其中储存器不与孢子的轨迹流体连通，并且储存器与孢子的轨迹流体连通的打开状态。 生物灭菌指示器系统可以包括生物灭菌指示器和适于联接到生物灭菌指示器的检测装置。 在一些实施方案中，该方法可以包括测定孢子以获得特征的可检测变化，并且检测基本上所有可检测的变化。
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公开(公告)号：US20130224849A1
公开(公告)日：2013-08-29
申请号：US13881741
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
IPC分类号： C12Q1/22
摘要： A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.
摘要翻译： 生物灭菌指示剂（BI）。 BI可以包括壳体和位于壳体中的容器。 容器可以容纳液体，容器的至少一部分可以是易碎的。 BI还可以包括第一室和第二室。 第二室可以包括至少一种生物活性来源。 BI还可以包括位于第一室和第二室之间的壳体中的衬底。 衬底可以定位成与第一腔室和第二腔室流体连通，并且衬底可以进一步定位成使得衬底不与生物活性源直接接触。
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公开(公告)号：US09322046B2
公开(公告)日：2016-04-26
申请号：US13881741
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
摘要： A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.
摘要翻译： 生物灭菌指示剂（BI）。 BI可以包括壳体和位于壳体中的容器。 容器可以容纳液体，容器的至少一部分可以是易碎的。 BI还可以包括第一室和第二室。 第二室可以包括至少一种生物活性来源。 BI还可以包括位于第一室和第二室之间的壳体中的衬底。 衬底可以定位成与第一腔室和第二腔室流体连通，并且衬底可以进一步定位成使得衬底不与生物活性源直接接触。
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公开(公告)号：US20130210048A1
公开(公告)日：2013-08-15
申请号：US13825143
申请日：2011-10-28
摘要： A method of detecting a biological activity is provided. The method includes contacting; in a liquid medium selected to facilitate a predetermined biological activity; a sample, an indicator reagent, and a substrate that receives and concentrates a biological derivative of the indicator reagent. The method further includes observing a portion of the substrate to detect the biological derivative. The method can be used to detect the presence or absence of a target cell.
摘要翻译： 提供了检测生物活性的方法。 该方法包括接触; 在选择用于促进预定生物活性的液体介质中; 样品，指示剂试剂和接收并浓缩指示剂试剂的生物衍生物的底物。 该方法还包括观察基底的一部分以检测生物衍生物。 该方法可用于检测靶细胞的存在或不存在。
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公开(公告)号：US08980622B2
公开(公告)日：2015-03-17
申请号：US13384886
申请日：2010-07-06
摘要： A biological sterilization indicator (BI) and a method of using same for assaying the lethality of a sterilization process. The BI can include a housing, which can include a first portion, and a second portion, which can be movable with respect to the first portion between a first and second position. The BI can further include a frangible container comprising a liquid. The BI can further include a spore reservoir and a projection positioned in the housing. The projection can be configured to fracture the container when the second portion of the housing is moved from the first position to the second position. The method can include maintaining a minimal cross-sectional area of space around the container when the second portion of the housing is in the first position, and fracturing the container in response to moving the second portion between the first and second positions.
摘要翻译： 生物灭菌指示剂（BI）及其用于测定灭菌过程的致死性的方法。 BI可以包括可以包括第一部分的壳体和可以在第一和第二位置之间相对于第一部分移动的第二部分。 BI可进一步包括包含液体的易碎容器。 BI还可以包括孢子贮存器和位于壳体中的突起。 当壳体的第二部分从第一位置移动到第二位置时，突起可被构造成使容器破裂。 该方法可以包括当壳体的第二部分处于第一位置时保持围绕容器的最小的空间横截面面积，并响应于在第一和第二位置之间移动第二部分而破裂容器。
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公开(公告)号：US20130217107A1
公开(公告)日：2013-08-22
申请号：US13821647
申请日：2011-10-28
申请人： Jeffrey C. Pederson , Sailaja Chandrapati , Bryan S. Behun , Barry W. Robole , Leroy J. Longworth , Kaileen Chen
发明人： Jeffrey C. Pederson , Sailaja Chandrapati , Bryan S. Behun , Barry W. Robole , Leroy J. Longworth , Kaileen Chen
IPC分类号： C12Q1/22
摘要： A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The reading apparatus can detect at least one of (i) when the well is empty; (ii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the first position, and (iii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the second position. The method can include detecting at least one of the above conditions, which can be used to detect an activation status of the biological sterilization indicator.
摘要翻译： 一种生物灭菌指标（BI）系统及方法。 该系统可以包括BI和包括井的读取装置。 BI可以包括壳体，其可以包括第一部分，以及可在第一“未激活”位置和第二“激活”位置之间移动的第二部分。 读取装置可以检测（i）当井是空的时候的至少一个; （ii）当所述生物灭菌指示器位于所述井中时，所述壳体的第二部分位于所述第一位置，以及（iii）当所述生物灭菌指示器位于所述井中时，所述壳体的第二部分处于所述第二位置 。 该方法可以包括检测可用于检测生物灭菌指示剂的活化状态的上述条件中的至少一个。
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公开(公告)号：US20130210069A1
公开(公告)日：2013-08-15
申请号：US13881065
申请日：2011-10-28
申请人： Jeffrey C. Pederson , Sailaja Chandrapati , Bryan S. Behun , Barry W. Robole , Leroy J. Longworth
发明人： Jeffrey C. Pederson , Sailaja Chandrapati , Bryan S. Behun , Barry W. Robole , Leroy J. Longworth
IPC分类号： C12Q1/02
摘要： A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.
摘要翻译： 一种生物灭菌指标（BI）系统及方法。 该系统可以包括BI和包括井的读取装置。 BI可以包括壳体，其可以包括第一部分，以及可在第一“未激活”位置和第二“激活”位置之间移动的第二部分。 BI还可以包括容纳液体并且尺寸设置在壳体中的易碎容器。 读取装置可以被配置为检测生物灭菌指示器的激活，例如通过检测第二部分处于第二位置，和/或通过检测来自易碎容器的液体存在于 生物灭菌指标。 该方法可以包括将BI定位在读取装置的阱中并检测激活，例如通过检测上述一个或多个条件。
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