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公开(公告)号：US08802392B2
公开(公告)日：2014-08-12
申请号：US13825881
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
IPC分类号： C12Q1/22
摘要： The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.
摘要翻译： 本发明提供了检测预定生物活性的方法。 该方法包括使用包含具有第一吸收光谱的第一指示剂和第二指示剂试剂的含水混合物。 第二指示剂通过预定的生物活性转化为具有第二发射光谱的第二生物衍生物。 第一吸收光谱包括在第二发射光谱中存在的波长的至少一部分中的可检测吸光度。 第一指示剂被含水液体接收并通过底物浓缩，便于检测第二生物衍生物。
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公开(公告)号：US20130210067A1
公开(公告)日：2013-08-15
申请号：US13825881
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Kurt J. Halverson , Brian R. Engel
IPC分类号： G01N21/64
摘要： The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.
摘要翻译： 本发明提供了检测预定生物活性的方法。 该方法包括使用包含具有第一吸收光谱的第一指示剂和第二指示剂试剂的含水混合物。 第二指示剂通过预定的生物活性转化为具有第二发射光谱的第二生物衍生物。 第一吸收光谱包括在第二发射光谱中存在的波长的至少一部分中的可检测吸光度。 第一指示剂被含水液体接收并通过底物浓缩，便于检测第二生物衍生物。
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公开(公告)号：US20110182770A1
公开(公告)日：2011-07-28
申请号：US13063945
申请日：2009-10-12
申请人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
发明人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
IPC分类号： A61L2/18
摘要： A biological sterilization indicator, system, and methods of determining the effectiveness of a sterilization process. The biological sterilization indicator can include a locus of spores, a reservoir containing a liquid, and a sterilant path positioned to provide fluid communication between ambience and the locus of spores. The reservoir can have a closed state in which the reservoir is not in fluid communication with the locus of spores and an open state in which the reservoir is in fluid communication with the locus of spores. The biological sterilization indicator system can include the biological sterilization indicator and a detection device adapted to be coupled to the biological sterilization indicator. In some embodiments, the method can include assaying the spores for a detectable change in a characteristic, and detecting substantially all of the detectable change.
摘要翻译： 一种生物灭菌指标，系统和确定灭菌过程有效性的方法。 生物灭菌指示剂可以包括孢子的位点，含有液体的储存器和定位成在气氛和孢子的位置之间提供流体连通的消毒剂通道。 储存器可以具有闭合状态，其中储存器不与孢子的轨迹流体连通，并且储存器与孢子的轨迹流体连通的打开状态。 生物灭菌指示器系统可以包括生物灭菌指示器和适于联接到生物灭菌指示器的检测装置。 在一些实施方案中，该方法可以包括测定孢子以获得特征的可检测变化，并且检测基本上所有可检测的变化。
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公开(公告)号：US08969029B2
公开(公告)日：2015-03-03
申请号：US13063945
申请日：2009-10-12
申请人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
发明人： Sailaja Chandrapati , Jeffrey C. Pederson , Heather M. Webb , Jeffrey D. Smith , Kurt J. Halverson , Bryan S. Behun , Peter D. Ludowise
摘要： A biological sterilization indicator, system, and methods of determining the effectiveness of a sterilization process. The biological sterilization indicator can include a locus of spores, a reservoir containing a liquid, and a sterilant path positioned to provide fluid communication between ambience and the locus of spores. The reservoir can have a closed state in which the reservoir is not in fluid communication with the locus of spores and an open state in which the reservoir is in fluid communication with the locus of spores. The biological sterilization indicator system can include the biological sterilization indicator and a detection device adapted to be coupled to the biological sterilization indicator. In some embodiments, the method can include assaying the spores for a detectable change in a characteristic, and detecting substantially all of the detectable change.
摘要翻译： 一种生物灭菌指标，系统和确定灭菌过程有效性的方法。 生物灭菌指示剂可以包括孢子的位点，含有液体的储存器和定位成在气氛和孢子的位置之间提供流体连通的消毒剂通道。 储存器可以具有闭合状态，其中储存器不与孢子的轨迹流体连通，并且储存器与孢子的轨迹流体连通的打开状态。 生物灭菌指示器系统可以包括生物灭菌指示器和适于联接到生物灭菌指示器的检测装置。 在一些实施方案中，该方法可以包括测定孢子以获得特征的可检测变化，并且检测基本上所有可检测的变化。
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公开(公告)号：US20130224849A1
公开(公告)日：2013-08-29
申请号：US13881741
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
IPC分类号： C12Q1/22
摘要： A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.
摘要翻译： 生物灭菌指示剂（BI）。 BI可以包括壳体和位于壳体中的容器。 容器可以容纳液体，容器的至少一部分可以是易碎的。 BI还可以包括第一室和第二室。 第二室可以包括至少一种生物活性来源。 BI还可以包括位于第一室和第二室之间的壳体中的衬底。 衬底可以定位成与第一腔室和第二腔室流体连通，并且衬底可以进一步定位成使得衬底不与生物活性源直接接触。
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6.发明申请Biological Compositions, Articles and Methods for Monitoring Sterilization Processes 审中-公开生物成分，用于监测灭菌过程的文章和方法公开(公告)号：US20110200992A1
公开(公告)日：2011-08-18
申请号：US13123974
申请日：2009-10-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/22
摘要： A sterility indicating composition comprising a plurality of sterilization process resistant spores; a germination medium comprising a sub-lethal amount of at least one cell-permeant nucleic acid-interacting fluorescent dye and at least one nutrient for germination of the spores; wherein the at least one cell-permeant fluorescent dye can interact with nucleic acids present in and produced by the plurality of spores during germination or during germination and outgrowth of the spores to produce an increase in fluorescence intensity, indicating that viable spores are present, and wherein the cell-permeant fluorescent dye is sufficiently stable at least at a temperature for incubating the spores to produce the increase in fluorescence intensity, a sterilization process indicator comprising the composition, and a method of determining the effectiveness of a sterilization process using the composition and indicator are disclosed.
摘要翻译： 一种无菌指示组合物，其包含多个耐灭菌过程的孢子; 包含亚致死量的至少一种细胞渗透性核酸相互作用荧光染料和至少一种用于孢子发芽的营养物的发芽培养基; 其中所述至少一种细胞透过性荧光染料可以在发芽过程中或在芽孢的发芽和生长期间与存在于和由多个孢子产生的核酸相互作用以产生荧光强度的增加，从而表明存在可行的孢子，以及 其中所述细胞透过性荧光染料至少在用于培养孢子以产生荧光强度增加的温度下是足够稳定的，包含该组合物的灭菌过程指示剂以及使用该组合物确定灭菌过程的有效性的方法，以及 指标。
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公开(公告)号：US09322046B2
公开(公告)日：2016-04-26
申请号：US13881741
申请日：2011-10-28
申请人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
发明人： Sailaja Chandrapati , Heather M. Webb , Jeffrey C. Pederson , Jeffrey D. Smith , RuthAnn R. Duda , Brian J. Engel
摘要： A biological sterilization indicator (BI). The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a substrate positioned in the housing between the first chamber and the second chamber. The substrate can be positioned in fluid communication with the first chamber and the second chamber, and the substrate can be further positioned such that the substrate is not in direct contact with the source of biological activity.
摘要翻译： 生物灭菌指示剂（BI）。 BI可以包括壳体和位于壳体中的容器。 容器可以容纳液体，容器的至少一部分可以是易碎的。 BI还可以包括第一室和第二室。 第二室可以包括至少一种生物活性来源。 BI还可以包括位于第一室和第二室之间的壳体中的衬底。 衬底可以定位成与第一腔室和第二腔室流体连通，并且衬底可以进一步定位成使得衬底不与生物活性源直接接触。
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公开(公告)号：US20130210048A1
公开(公告)日：2013-08-15
申请号：US13825143
申请日：2011-10-28
摘要： A method of detecting a biological activity is provided. The method includes contacting; in a liquid medium selected to facilitate a predetermined biological activity; a sample, an indicator reagent, and a substrate that receives and concentrates a biological derivative of the indicator reagent. The method further includes observing a portion of the substrate to detect the biological derivative. The method can be used to detect the presence or absence of a target cell.
摘要翻译： 提供了检测生物活性的方法。 该方法包括接触; 在选择用于促进预定生物活性的液体介质中; 样品，指示剂试剂和接收并浓缩指示剂试剂的生物衍生物的底物。 该方法还包括观察基底的一部分以检测生物衍生物。 该方法可用于检测靶细胞的存在或不存在。
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公开(公告)号：US08647574B2
公开(公告)日：2014-02-11
申请号：US12743252
申请日：2008-11-19
申请人： Kurt J. Halverson , Stephen C. P. Joseph , Raj Rajagopal , David J. Velasquez , Cynthia D. Zook , Sailaja Chandrapati , Daniel E. Siltberg , Paul J. Cobian , Theresa J. Gerten
发明人： Kurt J. Halverson , Stephen C. P. Joseph , Raj Rajagopal , David J. Velasquez , Cynthia D. Zook , Sailaja Chandrapati , Daniel E. Siltberg , Paul J. Cobian , Theresa J. Gerten
IPC分类号： G01N15/06
CPC分类号： G01N1/38 , G01N1/4077 , G01N2001/4088
摘要： A system and method for preparing and collecting samples for analyte testing. The system can include a sample preparation system and a sample collection system coupled to the sample preparation system. The sample preparation system can include at least one of a deformable self-supporting receptacle comprising a reservoir and a freestanding receptacle comprising a reservoir. The reservoir can be adapted to contain a liquid composition. The sample collection system can be positioned in fluid communication with a reservoir of the sample preparation system, and can be adapted to capture an analyte of interest. The method can include providing a fluid path defined at least partially by the sample preparation system and the sample collection system, positioning the liquid composition in a reservoir of the sample preparation system, and moving at least a portion of the liquid composition in the fluid path to the sample collection system.
摘要翻译： 用于制备和收集样品用于分析物测试的系统和方法。 该系统可以包括与样品制备系统耦合的样品制备系统和样品收集系统。 样品制备系统可以包括可变形的自支撑容器中的至少一个，其包括储存器和包括储存器的独立容器。 储存器可以适于容纳液体组合物。 样品收集系统可以定位成与样品制备系统的储存器流体连通，并且可以适于捕获感兴趣的分析物。 该方法可以包括提供至少部分地由样品制备系统和样品收集系统限定的流体路径，将液体组合物定位在样品制备系统的储存器中，并且将至少一部分液体组合物移动到流体路径 到采样系统。
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公开(公告)号：US20100248215A1
公开(公告)日：2010-09-30
申请号：US12743449
申请日：2008-11-19
申请人： Kurt J. Halverson , Stephen C.P. Joseph , Raj Rajagopal , Matthew D. Reier , David J. Velasquez , Cynthia D. Zook , Sailaja Chandrapati
发明人： Kurt J. Halverson , Stephen C.P. Joseph , Raj Rajagopal , Matthew D. Reier , David J. Velasquez , Cynthia D. Zook , Sailaja Chandrapati
CPC分类号： G01N1/38
摘要： A system and method for preparing and delivering samples for analyte testing. The system can include a sample preparation system and a sample delivery system coupled to the sample preparation system. The sample preparation system can include a deformable self-supporting receptacle comprising a reservoir adapted to contain a liquid composition comprising a source and a diluent. The sample delivery system can include a valve positioned in fluid communication with the reservoir and adapted to control the removal of a sample from the sample preparation system. The method can include applying pressure to the deformable self-supporting receptacle to remove a sample from the sample preparation system via the sample delivery system.
摘要翻译： 用于制备和递送样品以进行分析物测试的系统和方法。 该系统可以包括与样品制备系统耦合的样品制备系统和样品递送系统。 样品制备系统可以包括可变形的自支撑容器，其包括适于容纳包含源和稀释剂的液体组合物的储存器。 样品输送系统可以包括定位成与储存器流体连通的阀，并适于控制样品从样品制备系统中的去除。 该方法可以包括向可变形的自支撑容器施加压力，以经由样品输送系统从样品制备系统移除样品。
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