公开(公告)号：US20070264695A1

公开(公告)日：2007-11-15

申请号：US11826459

申请日：2007-07-16

申请人： Satoshi Negishi ,  Junko Suzuki ,  Chika Sakurai ,  Shin Arimoto ,  Hidetaka Uehara ,  Tadashiro Hirose ,  Yoshie Yamauchi ,  Yuri Arai ,  Tomomi Suganuma

发明人： Satoshi Negishi ,  Junko Suzuki ,  Chika Sakurai ,  Shin Arimoto ,  Hidetaka Uehara ,  Tadashiro Hirose ,  Yoshie Yamauchi ,  Yuri Arai ,  Tomomi Suganuma

IPC分类号： C12P7/64 ,  C12N9/20

CPC分类号： C12N9/18

摘要： The present invention provides a method for producing a purified lipase which comprises steps of: (a) bringing a long chain fatty acid triglyceride and a medium-chain triglyceride into contact with a lipase to purify the lipase; and (b) collecting the purified lipase. According to this production method, the purified lipase can be obtained wherein content of impurity such as silicon is decreased.

摘要翻译： 本发明提供了一种纯化脂肪酶的制备方法，其特征在于，包括以下步骤：（a）使长链脂肪酸甘油三酯和中链甘油三酯与脂肪酶接触以纯化脂肪酶; 和（b）收集纯化的脂肪酶。 根据该制造方法，可以得到其中杂质如硅的含量降低的纯化的脂肪酶。

公开(公告)号：US20060105438A1

公开(公告)日：2006-05-18

申请号：US11320760

申请日：2005-12-30

申请人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai ,  Tadashiro Hirose ,  Hidetaka Uehara ,  Shin Arimoto ,  Tomomi Suganuma

发明人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai ,  Tadashiro Hirose ,  Hidetaka Uehara ,  Shin Arimoto ,  Tomomi Suganuma

IPC分类号： C12P7/64

CPC分类号： C12P7/6454 ,  C12N9/20

摘要： A method for producing a lipase powder, which comprises the step of spray drying an aqueous solution containing a lipase adjusted to pH 6 to 7.5; the lipase powder which is a 1,3-specific lipase derived from Rhizomucor miehei, spherical, and contains not more than 10% by weight of moisture; and a lipase composition wherein the lipase powder is immersed or impregnated in fatty oil. According to the present invention, a lipase powder of which 1,3-selectivity is improved can be provided.

摘要翻译： 一种生产脂肪酶粉末的方法，其包括将含有调节至pH6-7.5的脂肪酶的水溶液喷雾干燥的步骤; 所述脂肪酶粉末是来自根毛霉（Rhizomucor miehei）的1,3-特异性脂肪酶，为球形，并且含有不超过10重量％的水分; 脂肪酶组合物，其中将脂肪酶粉末浸渍或浸渍在脂肪油中。 根据本发明，可以提供改善1,3-选择性的脂肪酶粉末。

公开(公告)号：US20060115882A1

公开(公告)日：2006-06-01

申请号：US11326436

申请日：2006-01-06

申请人： Satoshi Negishi ,  Yuri Arai ,  Shin Arimoto ,  Hidetaka Uehara

发明人： Satoshi Negishi ,  Yuri Arai ,  Shin Arimoto ,  Hidetaka Uehara

IPC分类号： C12P7/64

CPC分类号： A23D9/00 ,  C11C3/10 ,  C12P7/6454 ,  C12P7/6472

摘要： A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride composed of a medium chain fatty acid and a long chain fatty acid as the constituting fatty acids in the step of the first reaction; transesterifying the reaction product with an alcohol monoester of the medium chain fatty acid in the presence of an sn-1,3-position specific enzyme in the step of the second reaction and then taking the alcohol monoester of the medium chain fatty acid and the alcohol monoester of the long chain fatty acid from the reaction product obtained in the step of the second reaction to obtain the symmetric triglyceride composed of the medium chain fatty acids at the sn-1, 3 positions and the long chain fatty acid at the sn-2 position. According to this method, highly pure symmetric triglycerides comprising a medium-chain fatty acid at sn-1 and 3 positions and a long chain fatty acid at sn-2 position can be industrially efficiently produced.

摘要翻译： 一种对称甘油三酯的制造方法，其特征在于，在酶或化学催化剂存在下使中链脂肪酸甘油三酯与长链脂肪酸甘油三酯反应，进行无规酯交换反应，得到含有 在第一反应步骤中由构成脂肪酸的中链脂肪酸和长链脂肪酸组成的甘油三酸酯; 在第二反应步骤中，在sn-1,3位特异性酶存在下，用中链脂肪酸的醇单酯将反应产物进行酯交换，然后取中链脂肪酸和醇的醇单酯 在第二反应步骤中获得的反应产物中的长链脂肪酸的单酯，得到由sn-1,3位置的中链脂肪酸组成的对称甘油三酯，sn-2处的长链脂肪酸 位置。 根据该方法，可以在工业上有效地制造包含sn-1和3位的中链脂肪酸和sn-2位的长链脂肪酸的高纯度对称甘油三酯。

公开(公告)号：US20090104680A1

公开(公告)日：2009-04-23

申请号：US12314473

申请日：2008-12-11

申请人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai

发明人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai

IPC分类号： C12N9/20

CPC分类号： C12P7/6454 ,  C12N9/20 ,  C12N9/96 ,  C12N9/98

摘要： A lipase powder which is a granulated substance containing a lipase and a solid content of animal milk, a lipase composition wherein said lipase powder is immersed or impregnated in fatty oil, and a method for producing the lipase powder which comprises the step of adding animal milk or cream derived from the animal milk to an aqueous solution containing a lipase, and the step of spray-drying, freeze-drying or solvent-precipitating the mixture thereof are provided. According to the present invention, a lipase powder of which lipase activity and stability are improved can be provided.

摘要翻译： 一种脂肪酶粉末，其是含有脂肪酶和动物乳固体含量的粒状物质，其中所述脂肪酶粉末浸渍或浸渍在脂肪油中的脂肪酶组合物，以及包含添加动物乳的步骤的方法 或来自动物乳的乳膏至含有脂肪酶的水溶液，并且提供其喷雾干燥，冷冻干燥或溶剂沉淀其混合物的步骤。 根据本发明，可以提供脂肪酶活性和稳定性提高的脂肪酶粉末。

公开(公告)号：US20060105935A1

公开(公告)日：2006-05-18

申请号：US11320756

申请日：2005-12-30

申请人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai

发明人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai

IPC分类号： C11D17/00

CPC分类号： C12P7/6454 ,  C12N9/20 ,  C12N9/96 ,  C12N9/98

摘要： A lipase powder which is a granulated substance containing a lipase and a solid content of animal milk, a lipase composition wherein said lipase powder is immersed or impregnated in fatty oil, and a method for producing the lipase powder which comprises the step of adding animal milk or cream derived from the animal milk to an aqueous solution containing a lipase, and the step of spray-drying, freeze-drying or solvent-precipitating the mixture thereof are provided. According to the present invention, a lipase powder of which lipase activity and stability are improved can be provided.

公开(公告)号：US08110386B2

公开(公告)日：2012-02-07

申请号：US12314473

申请日：2008-12-11

申请人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai

发明人： Junko Suzuki ,  Satoshi Negishi ,  Yuri Arai ,  Chika Sakurai

IPC分类号： C12N9/96

CPC分类号： C12P7/6454 ,  C12N9/20 ,  C12N9/96 ,  C12N9/98

摘要： A lipase powder which is a granulated substance containing a lipase and a solid content of animal milk, a lipase composition wherein said lipase powder is immersed or impregnated in fatty oil, and a method for producing the lipase powder which comprises the step of adding animal milk or cream derived from the animal milk to an aqueous solution containing a lipase, and the step of spray-drying, freeze-drying or solvent-precipitating the mixture thereof are provided. According to the present invention, a lipase powder of which lipase activity and stability are improved can be provided.

摘要翻译： 一种脂肪酶粉末，其是含有脂肪酶和动物乳固体含量的粒状物质，其中所述脂肪酶粉末浸渍或浸渍在脂肪油中的脂肪酶组合物，以及包含添加动物乳的步骤的方法 或来自动物乳的乳膏至含有脂肪酶的水溶液，并且提供其喷雾干燥，冷冻干燥或溶剂沉淀其混合物的步骤。 根据本发明，可以提供脂肪酶活性和稳定性提高的脂肪酶粉末。

公开(公告)号：US07442531B2

公开(公告)日：2008-10-28

申请号：US11326436

申请日：2006-01-06

申请人： Satoshi Negishi ,  Yuri Arai ,  Shin Arimoto ,  Hidetaka Uehara

发明人： Satoshi Negishi ,  Yuri Arai ,  Shin Arimoto ,  Hidetaka Uehara

IPC分类号： C12P7/64

CPC分类号： A23D9/00 ,  C11C3/10 ,  C12P7/6454 ,  C12P7/6472

摘要： A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride composed of a medium chain fatty acid and a long chain fatty acid as the constituting fatty acids in the step of the first reaction; transesterifying the reaction product with an alcohol monoester of the medium chain fatty acid in the presence of an sn-1,3-position specific enzyme in the step of the second reaction and then taking the alcohol monoester of the medium chain fatty acid and the alcohol monoester of the long chain fatty acid from the reaction product obtained in the step of the second reaction to obtain the symmetric triglyceride composed of the medium chain fatty acids at the sn-1, 3 positions and the long chain fatty acid at the sn-2 position. According to this method, highly pure symmetric triglycerides comprising a medium-chain fatty acid at sn-1 and 3 positions and a long chain fatty acid at sn-2 position can be industrially efficiently produced.

摘要翻译： 一种对称甘油三酯的制造方法，其特征在于，在酶或化学催化剂存在下使中链脂肪酸甘油三酯与长链脂肪酸甘油三酯反应，进行无规酯交换反应，得到含有 在第一反应步骤中由构成脂肪酸的中链脂肪酸和长链脂肪酸组成的甘油三酸酯; 在第二反应步骤中，在sn-1,3位特异性酶存在下，用中链脂肪酸的醇单酯将反应产物进行酯交换，然后取中链脂肪酸和醇的醇单酯 在第二反应步骤中获得的反应产物中的长链脂肪酸的单酯，得到由sn-1,3位置的中链脂肪酸组成的对称甘油三酯，sn-2处的长链脂肪酸 位置。 根据该方法，可以在工业上有效地制造包含sn-1和3位的中链脂肪酸和sn-2位的长链脂肪酸的高纯度对称甘油三酯。

公开(公告)号：US20070141692A1

公开(公告)日：2007-06-21

申请号：US11703219

申请日：2007-02-07

申请人： Tadashiro Hirose ,  Yuri Arai ,  Satoshi Negishi

发明人： Tadashiro Hirose ,  Yuri Arai ,  Satoshi Negishi

IPC分类号： C12N9/20

CPC分类号： C12P7/6472 ,  C12N9/20 ,  C12P7/6436 ,  C12P7/6454

摘要： There is provided a lipase composition comprising (a) a powdered lipase which is a lipase derived from Rhizomucor sp. or a powdered lipase which is a lipase derived from Penicillum sp. and (b) a powdered lipase selected from the group consisting of a powdered lipase which is a lipase derived from Alcaligenes sp., a powdered lipase which is a lipase derived from Rhizopus sp. and a powdered lipase which is a lipase derived from Thermomyces sp. When using this lipase composition, a compound(s) having at least one alcoholic hydroxyl group in the molecule can be effectively esterified with a carboxylic acid(s).

摘要翻译： 提供了脂肪酶组合物，其包含（a）粉状脂肪酶，其是源自根毛霉属（Rhizomucor sp。）的脂肪酶。 或粉状脂肪酶，其是来源于青霉属的脂肪酶。 和（b）粉末脂肪酶，其选自粉状脂肪酶，其是来自产碱杆菌属的脂肪酶，粉状脂肪酶，其是源自根霉属的脂肪酶。 和粉状脂肪酶，其是源自Thermomyces sp。的脂肪酶。 当使用该脂肪酶组合物时，分子中具有至少一个醇羟基的化合物可以用羧酸有效酯化。

公开(公告)号：US07811802B2

公开(公告)日：2010-10-12

申请号：US11703219

申请日：2007-02-07

申请人： Tadashiro Hirose ,  Yuri Arai ,  Satoshi Negishi

发明人： Tadashiro Hirose ,  Yuri Arai ,  Satoshi Negishi

IPC分类号： C12N9/20 ,  C12P7/64

CPC分类号： C12P7/6472 ,  C12N9/20 ,  C12P7/6436 ,  C12P7/6454

摘要： There is provided a lipase composition comprising (a) a powdered lipase which is a lipase derived from Rhizomucor sp. or a powdered lipase which is a lipase derived from Penicillium sp. and (b) a powdered lipase selected from the group consisting of a powdered lipase which is a lipase derived from Alcaligenes sp., a powdered lipase which is a lipase derived from Rhizopus sp. and a powdered lipase which is a lipase derived from Thermomyces sp. When using this lipase composition, a compound(s) having at least one alcoholic hydroxyl group in the molecule can be effectively esterified with a carboxylic acid(s).

摘要翻译： 提供了脂肪酶组合物，其包含（a）粉状脂肪酶，其是源自根毛霉属（Rhizomucor sp。）的脂肪酶。 或粉状脂肪酶，其是来源于青霉属的脂肪酶。 和（b）粉末脂肪酶，其选自粉状脂肪酶，其是来自产碱杆菌属的脂肪酶，粉状脂肪酶，其是源自根霉属的脂肪酶。 和粉状脂肪酶，其是源自Thermomyces sp。的脂肪酶。 当使用该脂肪酶组合物时，分子中具有至少一个醇羟基的化合物可以用羧酸有效酯化。