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    Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus
    5.
    发明申请
    Novel single nucleotide polymorphisms and community-associated methicillin-resistant staphylococcus aureus 有权
    新型单核苷酸多态性和社区相关耐甲氧西林金黄色葡萄球菌

    公开(公告)号：US20120009572A1

    公开(公告)日：2012-01-12

    申请号：US13068331

    申请日：2011-05-09

    申请人： Scott E. Gygax ,  Sean Chadwick ,  Aditya Prasad ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Scott E. Gygax ,  Sean Chadwick ,  Aditya Prasad ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q2600/156

    摘要： The present invention is based on the discovery of novel polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus. The presence of G88A and/or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.

    摘要翻译： 本发明基于在金黄色葡萄球菌中青霉素结合蛋白（pbp3）基因中新型多态性（SNP）的发现。 G88A和/或G2047A SNP的存在为耐甲氧西林金黄色葡萄球菌（MRSA），特别是社区相关MRSA（CA-MRSA）的存在提供了准确，可靠的生物标志物。 本发明提供用于检测SNP的试剂以及鉴定和使用这些变体筛选受试者存在CA-MRSA的方法。 所述方法包括从哺乳动物（优选人）分离生物样品并测试与CA-MRSA相关的pbp3基因中SNP的存在。

    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents
    8.
    发明授权
    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents 有权
    与之相关的方法和组合物用于检测和鉴定来自致病因子的不同种类的核酸

    公开(公告)号：US08932832B2

    公开(公告)日：2015-01-13

    申请号：US13199380

    申请日：2011-08-26

    申请人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

    发明人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

    IPC分类号： C07H21/04 ,  C12Q1/70

    CPC分类号： C12Q1/705

    摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

    摘要翻译： 在本文中描述了用于在单个容器中，例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法，所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物，从而在物种间限定PCR靶区域，并提供对靶区域内的核酸序列特异的第一寡核苷酸探针， 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针，其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记，进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签，从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的，例如，其中感染剂是疱疹病毒科家族的成员。