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    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents
    2.
    发明授权
    Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents 有权
    与之相关的方法和组合物用于检测和鉴定来自致病因子的不同种类的核酸

    公开(公告)号：US08932832B2

    公开(公告)日：2015-01-13

    申请号：US13199380

    申请日：2011-08-26

    申请人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

    发明人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

    IPC分类号： C07H21/04 ,  C12Q1/70

    CPC分类号： C12Q1/705

    摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

    摘要翻译： 在本文中描述了用于在单个容器中，例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法，所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物，从而在物种间限定PCR靶区域，并提供对靶区域内的核酸序列特异的第一寡核苷酸探针， 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针，其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记，进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签，从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的，例如，其中感染剂是疱疹病毒科家族的成员。

    Method of determining types I, II, III, IV or V or methicillin-resistant staphylococcus aureus (MRSA) in a biological sample
    5.
    发明申请
    Method of determining types I, II, III, IV or V or methicillin-resistant staphylococcus aureus (MRSA) in a biological sample 有权
    在生物样品中测定I，II，III，IV或V型或耐甲氧西林金黄色葡萄球菌（MRSA）的方法

    公开(公告)号：US20110312876A1

    公开(公告)日：2011-12-22

    申请号：US12930663

    申请日：2011-01-13

    申请人： Scott Elliot Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin E. Adelson ,  Eli Mordechai

    发明人： Scott Elliot Gygax ,  Christina Lim Overmyer ,  Lisa A. DeSalvia ,  Martin E. Adelson ,  Eli Mordechai

    IPC分类号： A61K38/14 ,  A61P31/04 ,  C12Q1/68 ,  A61K31/5377 ,  C40B30/00 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  A61K31/5377 ,  A61K38/12 ,  A61K38/14 ,  C12Q1/6844 ,  C12Q2600/16

    摘要： Disclosed are diagnostic methods for determining a subtype of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample of a mammal. Methods include providing a biological sample of the mammal, performing a PCR analysis of the biological sample, and analyzing the PCR amplicons with respect to their sizes so as to determine for type I, type II, type III, type IV or type V MRSA that may be present in the biological sample. Further example embodiments include using at least one mecA primer pair and/or using at least one Staphylococcus aureus nuc primer pair in the PCR analysis. Further disclosed are methods for screening populations for MRSA, and methods of treating a mammal testing positive for Type IV MRSA. Also disclosed are kits for determining a MRSA subtype in a mammal and isolated primers that may be used in the present methods and kits.

    摘要翻译： 公开了用于确定哺乳动物生物样品中耐甲氧西林金黄色葡萄球菌（MRSA）亚型的诊断方法。 方法包括提供哺乳动物的生物样品，对生物样品进行PCR分析，并根据其大小对PCR扩增子进行分析，以确定I型，II型，III型，IV型或V型MRSA， 可能存在于生物样品中。 另外的实施例包括在PCR分析中使用至少一个mecA引物对和/或使用至少一种金黄色葡萄球菌引物对。 进一步披露的是用于筛选MRSA群体的方法以及治疗IV型MRSA阳性的哺乳动物的方法。 还公开了用于测定哺乳动物中MRSA亚型的试剂盒和可用于本发明方法和试剂盒的分离的引物。

    Antibodies specific to antigens of bartonella henselae and use of these antigens in immunoassays
    8.
    发明申请
    Antibodies specific to antigens of bartonella henselae and use of these antigens in immunoassays 有权
    针对巴氏杆菌的抗原特异性抗体和在免疫测定中使用这些抗原的抗体

    公开(公告)号：US20080220458A1

    公开(公告)日：2008-09-11

    申请号：US12075036

    申请日：2008-03-07

    申请人： Tera L. McCool ,  Chien Chang Loa ,  Eli Mordechai ,  Martin E. Adelson

    发明人： Tera L. McCool ,  Chien Chang Loa ,  Eli Mordechai ,  Martin E. Adelson

    IPC分类号： G01N33/554 ,  G01N33/569

    CPC分类号： C07K16/12 ,  G01N33/56911

    摘要： Disclosed are antibodies that bind to the antigenic proteins GroES, RpIL, GroEL, SodB, UbiG, the ABC transporter, and an expressed antigenic protein of unknown function (the “BepA” protein) of Bartonella henselae, and use of these antigenic proteins in immunoassays in order to determine whether a sample from a subject contains one or more of these antibodies. Presence of such an antibody in the subject indicates that the subject is or was infected with Bartonella henselae, or indicates that the subject has an increased likelihood of being infected presently or in the past with Bartonella henselae. Also disclosed are kits for performing immunoassays, wherein each kit contains one or more of these antigenic proteins and also contains the reagents necessary for conducting an immunoassay.

    摘要翻译： 公开了结合抗原蛋白质GroES，RpIL，GroEL，SodB，UbiG，ABC转运蛋白和Bartonella henselae未知功能的表达抗原蛋白（“BepA”蛋白）的抗体，以及这些抗原蛋白在免疫测定中的用途 以确定来自受试者的样品是否含有这些抗体中的一种或多种。 受试者中存在这样的抗体表明受试者是或已被感染巴氏杆菌，或表明该受试者目前或过去与Bartonella henselae感染的可能性增加。 还公开了用于进行免疫测定的试剂盒，其中每个试剂盒含有这些抗原蛋白中的一种或多种，​​并且还含有进行免疫测定所必需的试剂。

    Methods and compositions for detecting serotypes of Chlamydia trachomatis capable of causing lymphogranuloma venereum
    9.
    发明申请
    Methods and compositions for detecting serotypes of Chlamydia trachomatis capable of causing lymphogranuloma venereum 有权
    用于检测能够引起淋巴性肉芽肿的沙眼衣原体血清型的方法和组合物

    公开(公告)号：US20070269810A1

    公开(公告)日：2007-11-22

    申请号：US11436506

    申请日：2006-05-18

    申请人： Jason Trama ,  Eli Mordechai ,  Martin E. Adelson

    发明人： Jason Trama ,  Eli Mordechai ,  Martin E. Adelson

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689

    摘要： Disclosed are methods and compositions for conducting assays of samples utilizing polymerase chain reactions (“PCRs”) in the detection of serotypes of Chlamydia trachomatis capable of causing lymphogranuloma venereum (“LGV”). These serotypes are in the L group of Chlamydia trachomatis, and include the L I, L II, and L III serotypes. These assays take advantage of a deletion occurring in the cytotoxin gene locus specific to the L I, L II, and L III serotypes. The genome of each of these three serotypes contains the nucleotide sequence of SEQ ID NO:1 and the nucleotide sequence of SEQ ID NO:2, wherein the nucleotide at the 3′ end of SEQ ID NO:1 and the nucleotide at the 5′ end of SEQ ID NO:2 are contiguous, and wherein the deletion point of the cytotoxin gene locus is located between these two nucleotides. Each of these assays employs a first primer having a nucleotide sequence flanking one side of the deletion point and a second primer having a nucleotide sequence flanking the other side of the deletion point, wherein the first primer and the second primer are capable of hybridizing respectively to the plus strand and the minus strand of the genome of Chlamydia trachomatis during the PCR. Synthesis during the PCR of a sequence-specific amplicon containing this deletion point indicates that the sample contains nucleic acid specific to an LGV-causing serotype of Chlamydia trachomatis. The amplicon can be detected during real-time PCR using a labeled oligonucleotide as a probe, or after end-point PCR by gel electrophoresis.

    摘要翻译： 公开了用于在检测能引起淋巴肉芽肿（“LGV”）的沙眼衣原体血清型中使用聚合酶链反应（“PCR”）进行测定的方法和组合物。 这些血清型是L组沙眼衣原体，包括L I，L II和L III血清型。 这些测定利用在L I，L II和L III血清型特异性的细胞毒素基因位点中发生的缺失。 这三种血清型中的每一种的基因组含有SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的核苷酸序列，其中SEQ ID NO：1的3'末端的核苷酸和5' SEQ ID NO：2的末端是连续的，并且其中细胞毒素基因位点的缺失点位于这两个核苷酸之间。 这些测定中的每一个采用具有位于缺失点的一侧侧翼的核苷酸序列的第一引物和具有位于缺失点另一侧侧翼的核苷酸序列的第二引物，其中第一引物和第二引物能够分别与 PCR期间沙眼衣原体基因组的正链和负链。 在含有该缺失点的序列特异性扩增子的PCR期间的合成表明样品含有对造成衣原体沙眼衣原体的导致LGV的血清型特异的核酸。 使用标记的寡核苷酸作为探针，或通过凝胶电泳进行终点PCR后，可以在实时PCR中检测扩增子。