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    METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM
    1.
    发明申请
    METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
    使用重组微生物生产聚酯共聚物的方法

    公开(公告)号：US20110104768A1

    公开(公告)日：2011-05-05

    申请号：US12989333

    申请日：2009-04-23

    申请人： Seiichi Taguchi ,  Kenji Tajima ,  Yasuharu Satoh ,  Ken'ichiro Matsumoto ,  Miwa Yamada ,  Shusei Obata ,  Hiromi Kambe ,  Katsuhiro Kohda ,  Katsuhiro Ohno ,  Takashi Shimamura

    发明人： Seiichi Taguchi ,  Kenji Tajima ,  Yasuharu Satoh ,  Ken'ichiro Matsumoto ,  Miwa Yamada ,  Shusei Obata ,  Hiromi Kambe ,  Katsuhiro Kohda ,  Katsuhiro Ohno ,  Takashi Shimamura

    IPC分类号： C12P7/62 ,  C12N1/00 ,  C12N1/21

    CPC分类号： C12P7/62

    摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

    摘要翻译： 本发明的目的是提供一种利用糖作为原料，通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。 提供以下：由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法，其特征在于，包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或 乳酸盐，能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质，能够催化乙酰乙酰辅酶A还原反应的蛋白质，以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质 含有碳源的培养基中的酸序列（a）或（b）：（a）从SEQ ID NO：2所示的氨基酸序列衍生的氨基酸序列，通过将位置130的至少一个氨基酸置换 ，325,477和481具有不同的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个 氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚酯共聚物。 根据本发明的制造方法，可以使用廉价的碳源作为原料，有效地制造由3HB和LA组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

    Method for production of polyester copolymer using recombinant microorganism
    2.
    发明授权
    Method for production of polyester copolymer using recombinant microorganism 有权
    使用重组微生物生产聚酯共聚物的方法

    公开(公告)号：US08563281B2

    公开(公告)日：2013-10-22

    申请号：US12989333

    申请日：2009-04-23

    申请人： Seiichi Taguchi ,  Kenji Tajima ,  Yasuharu Satoh ,  Ken'ichiro Matsumoto ,  Miwa Yamada ,  Shusei Obata ,  Hiromi Kambe ,  Katsunori Kohda ,  Katsuhiro Ohno ,  Takashi Shimamura

    发明人： Seiichi Taguchi ,  Kenji Tajima ,  Yasuharu Satoh ,  Ken'ichiro Matsumoto ,  Miwa Yamada ,  Shusei Obata ,  Hiromi Kambe ,  Katsunori Kohda ,  Katsuhiro Ohno ,  Takashi Shimamura

    IPC分类号： C12P7/62 ,  C12N1/20 ,  C12N1/00

    CPC分类号： C12P7/62

    摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.

    摘要翻译： 本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。 该方法可以包括培养表达：能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。 根据该制造方法，可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

    Method for producing polymer
    5.
    发明申请
    Method for producing polymer 审中-公开
    生产聚合物的方法

    公开(公告)号：US20090226988A1

    公开(公告)日：2009-09-10

    申请号：US12291779

    申请日：2008-11-13

    申请人： Kenji Tajima ,  Yasuharu Satou ,  Masanobu Munekata ,  Seiichi Taguchi ,  Tokuo Matsushima

    发明人： Kenji Tajima ,  Yasuharu Satou ,  Masanobu Munekata ,  Seiichi Taguchi ,  Tokuo Matsushima

    IPC分类号： C12P7/62 ,  C08G63/02

    CPC分类号： C12P7/625

    摘要： A method for producing a polymer including a chemical thioester exchange reaction for forming an acetyl-CoA by reacting an acetyl-thioester with CoA, a monomer-producing reaction for forming a (monomer precursor)-CoA derivative by reacting at least one monomer precursor compound with the acetyl-CoA and a polymerization reaction for forming the polymer comprising units of the monomer by polymerizing the (monomer precursor)-CoA derivative.

    摘要翻译： 一种制备聚合物的方法，所述聚合物包括通过使乙酰基 - 硫酯与CoA反应形成乙酰辅酶A的化学硫酯交换反应，用于形成（单体前体）-CoA衍生物的单体制备反应，使至少一种单体前体化合物 与乙酰辅酶A和通过聚合（单体前体）-CoA衍生物形成包含单体单元的聚合物的聚合反应。

    Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same
    7.
    发明申请
    Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same 失效
    能够产生聚羟基链烷酸酯，聚羟基链烷酸酯合成酶的微生物和编码它们的基因

    公开(公告)号：US20100035313A1

    公开(公告)日：2010-02-11

    申请号：US12459941

    申请日：2009-07-09

    申请人： Yasuharu Satou ,  Kenji Tajima ,  Masanobu Munekata ,  Tokuo Matsushima

    发明人： Yasuharu Satou ,  Kenji Tajima ,  Masanobu Munekata ,  Tokuo Matsushima

    IPC分类号： C12P7/62 ,  C12N1/00 ,  C12N1/20 ,  C07H21/00 ,  C12N15/63 ,  C12N9/00

    CPC分类号： C12P7/625 ,  C12N9/1029

    摘要： Problem to be Solved: To provide a new microorganism capable of producing a polyhydroxyalkanoate (PHA), a PHA synthase gene, an expression cassette including the gene, a vector including the expression cassette, a transformant transformed by the vector, a polypeptide having PHA synthase activity, a method for producing a PHA synthase and a method for producing a PHA.Solution: The new microorganism is capable of producing a polyhydroxyalkanoate comprising a 16S rRNA gene whose polynucleotide sequence shows 99% or more homology to a polynucleotide sequence represented by SEQ ID No: 1, having an optimum temperature of an activity temperature range for the growth and PHA production of the microorganism of at least 45° C. and being capable of growing at a pH range from 6 to 10.

    摘要翻译： 要解决的问题：提供能够产生聚羟基链烷酸酯（PHA），PHA合酶基因，包含该基因的表达盒，包含表达盒的载体，由载体转化的转化体的新微生物，具有PHA合酶的多肽 活性，生产PHA合成酶的方法和制备PHA的方法。 解决方案：新的微生物能够生产包含16S rRNA基因的聚羟基链烷酸酯，其多核苷酸序列与SEQ ID No：1所代表的多核苷酸序列具有99％或更高的同源性，具有生长活性温度范围的最适温度， PHA生产至少45℃的微生物，并能够在6至10的pH范围内生长。

    Methods for Purifying Amino Acids
    8.
    发明申请
    Methods for Purifying Amino Acids 有权
    纯化氨基酸的方法

    公开(公告)号：US20090130723A1

    公开(公告)日：2009-05-21

    申请号：US12282815

    申请日：2007-03-15

    申请人： Hideki Murata ,  Noboru Fujii ,  Kenji Tajima

    发明人： Hideki Murata ,  Noboru Fujii ,  Kenji Tajima

    IPC分类号： C12P13/24

    CPC分类号： C12P13/24 ,  B01J39/05

    摘要： In the method for separating and purifying histidine from a culture containing the amino acid, the culture containing histidine and microbial cells is charged onto the top of a column filled with a carrier particle whose particle size is 350 μm or more and which has an ability to adsorb histidine and then an eluent is passed through the column whereby accomplishing the separation and purification of histidine, and preferably in the step mentioned above, a strong acid cation exchange resin is employed as a carrier particle whereby accomplishing the separation and purification of histidine.

    摘要翻译： 在从含有氨基酸的培养物分离和纯化组氨酸的方法中，将含有组氨酸和微生物细胞的培养物装入填充有粒度为350μm以上的载体颗粒的柱的顶部，并且具有 吸附组氨酸，然后使洗脱液通过柱，从而完成组氨酸的分离和纯化，优选在上述步骤中，使用强酸阳离子交换树脂作为载体颗粒，从而完成组氨酸的分离和纯化。