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    Horseshoe crab amebocyte lysate factor G activation inhibitor
    2.
    发明授权
    Horseshoe crab amebocyte lysate factor G activation inhibitor 失效
    HORSESHOE CRAB AMEBOCYTE LYSATE因子G激活抑制剂

    公开(公告)号：US5155032A

    公开(公告)日：1992-10-13

    申请号：US474057

    申请日：1990-05-01

    申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

    发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

    IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579

    CPC分类号： C08B37/0024 ,  C07H3/06 ,  G01N33/579

    摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

    摘要翻译： PCT No.PCT / JP89 / 00903 Sec。 371日期1990年5月1日 102（e）日期1990年5月1日PCT提交1989年9月1日PCT公布。 公开号WO90 / 02951 日本3月22日，1990年。本发明涉及一种马蹄形蟹细胞裂解物因子G激活抑制剂，其包含含有至少一种由（2）〜（1→3）-β-D-葡萄糖苷结构部分组成的多糖苷的多糖苷作为活性成分 到具有连续结合的下式（IMAGE）的370（1-> 3）-β-D-葡萄糖苷结构单元。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

    Intramolecularly crosslinked (1.fwdarw.3)-.beta.-D-glucans
    4.
    发明授权
    Intramolecularly crosslinked (1.fwdarw.3)-.beta.-D-glucans 失效
    分子内交联（1-> 3）-β-D-葡聚糖

    公开(公告)号：US5574023A

    公开(公告)日：1996-11-12

    申请号：US369321

    申请日：1995-01-06

    申请人： Yuko Shibata ,  Jun Aketagawa ,  Shigenori Tanaka

    发明人： Yuko Shibata ,  Jun Aketagawa ,  Shigenori Tanaka

    IPC分类号： A61K31/715 ,  A61P35/00 ,  C08B37/00 ,  C12P19/04 ,  C12R1/645 ,  A01N43/04 ,  C07G3/00

    CPC分类号： C08B37/0024 ,  C12P19/04

    摘要： The invention stabilizes single helix conformation (1.fwdarw.3)-.beta.-D-glucans by intramolecular crosslinking and provides (1.fwdarw.3)-.beta.-D-glucans showing stable biological activities. Thus, intramolecularly crosslinked (1.fwdarw.3)-.beta.-D-glucans having a stable single helix conformation are produced by introducing a functional group into hydroxyl groups of (1.fwdarw.3)-.beta.-D-glucans, then causing the thus-obtained functional glucans to take a single helix conformation, and subjecting these, either as such or after binding them to a receptor therefor, to intramolecular crosslinking between the functional groups introduced, if necessary followed by releasing the receptor therefrom.

    摘要翻译： 本发明通过分子内交联稳定单螺旋构象（1-> 3）-β-D-葡聚糖，并提供显示稳定生物活性的（1-> 3）-β-D-葡聚糖。 因此，通过将官能团引入到（1-> 3）-β-D-葡聚糖的羟基中而产生具有稳定的单螺旋构象的分子内交联的（1-> 3）-β-D-葡聚糖， 获得的功能性葡聚糖以采取单一的螺旋构象，并且如果需要的话，将这些，或者将它们与它们的受体结合后进行引入的官能团之间的分子内交联，然后释放其受体。

    Method of preparing Limulus amoebocyte lysate
    5.
    发明授权
    Method of preparing Limulus amoebocyte lysate 失效
    制备鲎变形细胞裂解物的方法

    公开(公告)号：US5401647A

    公开(公告)日：1995-03-28

    申请号：US859411

    申请日：1992-05-27

    申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Yuko Shibata

    发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Yuko Shibata

    IPC分类号： G01N33/579 ,  C12N11/06 ,  C12N11/08 ,  C12N11/14 ,  C12Q1/37

    CPC分类号： G01N33/579 ,  Y10T436/25125

    摘要： Disclosed is a method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1.fwdarw.3)-.beta.-D-glucoside structural portion represented by the following formula [I] produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized: ##STR1## wherein n represents an integer of 2 to 370.

    摘要翻译： PCT No.PCT / JP91 / 01308 371日期：1992年5月27日 102（e）日期1992年5月27日PCT 1991年9月27日PCT PCT。 公开号WO92 / 06381 日期：1992年4月16日。公开是一种制备基本上不含因子G的鲎变形细胞裂解物的方法，其包括使鲎变形细胞裂解物与其上含有（1-> 3）-β-D-葡萄糖苷结构部分的不溶性载体接触 由通过解聚和/或分馏碳水化合物链产生的下式[I]表示的化合物：其中n表示2至370的整数。

    Endotoxin stabilizing agent, endotoxin composition and method for assaying endotoxin
    6.
    发明授权
    Endotoxin stabilizing agent, endotoxin composition and method for assaying endotoxin 失效
    内毒素稳定剂，内毒素组成和测定内毒素的方法

    公开(公告)号：US5876955A

    公开(公告)日：1999-03-02

    申请号：US774236

    申请日：1996-12-27

    申请人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

    发明人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

    IPC分类号： G01N33/579 ,  C12Q1/34 ,  C12N9/99 ,  C12Q1/37

    CPC分类号： G01N33/579

    摘要： An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

    摘要翻译： 提供了一种内毒素稳定剂，其可用于将试样或参考标准品的内毒素活性长时间维持在稳定状态，并制备具有减小的间隙变化的内毒素参照标准物，其对于 很长一段时间的形式，特别是一个解决方案，并承受反复使用。 还提供了包含上述内毒素稳定剂和内毒素的内毒素组合物和使用该内毒素测定内毒素的方法。 所述内毒素稳定剂包括：（1）选自氨基醇，多元醇，非离子表面活性剂，多糖和螯合剂中的至少一种物质; （2）至少氨基醇和多元醇; （3）多元醇优选为甘油或其衍生物; （4）除了组分（1）至（3）之外的聚乙二醇作为有效成分; 或（5）至少聚蔗糖，以及碱土金属盐和/或聚乙二醇作为有效成分。 内毒素组合物包含上述内毒素稳定剂。 用于测定内毒素的方法包括向样品中加入上述内毒素稳定剂，从而稳定样品中内毒素的活性。

    Endotoxin stabilizing agent, endotoxin composition and method for assaying endotoxin
    7.
    发明授权
    Endotoxin stabilizing agent, endotoxin composition and method for assaying endotoxin 失效
    内毒素稳定剂，内毒素组成和测定内毒素的方法

    公开(公告)号：US5648230A

    公开(公告)日：1997-07-15

    申请号：US474722

    申请日：1995-06-07

    申请人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

    发明人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

    IPC分类号： G01N33/579 ,  C12Q1/34

    CPC分类号： G01N33/579

    摘要： An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

    摘要翻译： 提供了一种内毒素稳定剂，其可用于将试样或参考标准品的内毒素活性长时间维持在稳定状态，并制备具有减小的间隙变化的内毒素参照标准物，其对于 很长一段时间的形式，特别是一个解决方案，并承受反复使用。 还提供了包含上述内毒素稳定剂和内毒素的内毒素组合物和使用该内毒素测定内毒素的方法。 所述内毒素稳定剂包括：（1）选自氨基醇，多元醇，非离子表面活性剂，多糖和螯合剂中的至少一种物质; （2）至少氨基醇和多元醇; （3）多元醇优选为甘油或其衍生物; （4）除了组分（1）至（3）之外的聚乙二醇作为有效成分; 或（5）至少聚蔗糖，以及碱土金属盐和/或聚乙二醇作为有效成分。 内毒素组合物包含上述内毒素稳定剂。 用于测定内毒素的方法包括向样品中加入上述内毒素稳定剂，从而稳定样品中内毒素的活性。

    Reagent for determining (1.fwdarw.3)-.beta.-D-glucan
    8.
    发明授权
    Reagent for determining (1.fwdarw.3)-.beta.-D-glucan 失效
    测定（1-> 3）-β-D-葡聚糖的试剂

    公开(公告)号：US5681710A

    公开(公告)日：1997-10-28

    申请号：US391097

    申请日：1995-02-21

    申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

    发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

    IPC分类号： G01N33/579 ,  C12Q1/56 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/44

    CPC分类号： G01N33/579 ,  G01N2400/22 ,  G01N2400/24

    摘要： The present invention provides a reagent for determining (1.fwdarw.3)-.beta.-D-glucan comprising a lysate substantially free from any endotoxin-sensitive factor, which is obtained by contacting limulus amebocyte lysate optionally containing dextran with a polyamide or cellulose insoluble carrier, which makes it possible to rapidly and easily determine at a high accuracy (1.fwdarw.3)-.beta.-D-glucan of mycotic origin contained in the body fluid such as blood or urine.

    摘要翻译： 本发明提供了一种用于测定（1→3）-β-D-葡聚糖的试剂，其包含基本上不含任何内毒素敏感因子的裂解物，所述裂解物通过任选地含有葡聚糖的鲎变形细胞裂解物与聚酰胺或纤维素不溶性载体 ，这使得可以以高精度（1→3）-β-D-葡聚糖快速和容易地测定包含在体液如血液或尿液中的真菌来源。

    Reagent for endotoxin-specific assay
    9.
    发明授权
    Reagent for endotoxin-specific assay 失效
    内毒素特异性检测试剂

    公开(公告)号：US5550030A

    公开(公告)日：1996-08-27

    申请号：US120469

    申请日：1993-09-14

    申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

    发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

    IPC分类号： G03C5/50 ,  G01N33/545 ,  G01N33/548 ,  G01N33/579 ,  G03C7/407 ,  C12Q1/37 ,  C12N11/08 ,  C12N11/12 ,  C12Q1/34

    CPC分类号： G01N33/579

    摘要： Disclosed are a reagent for endotoxin-specific assay which comprises an insoluble carrier having immobilized thereon at least an endotoxin-sensitive factor derived from a limulus amebocyte; a kit for endotoxin-specific assay containing said reagent and a substrate for activated factor C or a substrate for clotting enzyme; a method for assaying endotoxin comprising applying a sample solution to said reagent to cause endotoxin in the sample to react with factor C in said reagent and determining a change of a substrate; and a process for preparing said reagent which comprises physically or chemically immobilizing at least an endotoxin-sensitive factor derived from a limulus amebocyte on an insoluble carrier. Endotoxin in a sample, even turbid or colored, can be specifically assayed with ease and rapidness without the influence of a (1.fwdarw.3)-.beta.-glucan.

    摘要翻译： 公开了一种用于内毒素特异性测定的试剂，其包含其上固定有至少一种源自鲎变形细胞的内毒素敏感因子的不溶性载体; 含有所述试剂和活化因子C的底物或用于凝固酶的底物的内毒素特异性测定试剂盒; 一种用于测定内毒素的方法，包括将样品溶液施加到所述试剂以使样品中的内毒素与所述试剂中的因子C反应并确定底物的变化; 以及制备所述试剂的方法，其包括在不溶性载体上物理或化学地固定来自鲎变形细胞的至少一种内毒素敏感因子。 样品中的内毒素，甚至浑浊或有色，可以在不受（1-> 3）-β-葡聚糖的影响的情况下容易且快速地进行特异性测定。

    Method of measuring lipoarabinomannan and application thereof
    10.
    发明授权
    Method of measuring lipoarabinomannan and application thereof 有权
    测量脂多巴胺甘露聚糖的方法及其应用

    公开(公告)号：US07851179B2

    公开(公告)日：2010-12-14

    申请号：US10584071

    申请日：2004-12-22

    申请人： Shigenori Tanaka ,  Shoji Takahashi

    发明人： Shigenori Tanaka ,  Shoji Takahashi

    IPC分类号： C12Q1/04

    CPC分类号： G01N33/579 ,  G01N33/56911 ,  G01N2400/50

    摘要： A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing a Limulus reagent to contact with a LAM-containing sample, a method for removing reactivity of LAM with a Limulus reagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using a Limulus reagent which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the described-described removing method; and the like.

    摘要翻译： 一种用于测量LAM的方法和一种用于检测耐酸细菌的方法，其包括使鲎试剂与含LAM的样品接触的步骤，用于除去LAM与鲎试剂的反应性的方法，所述方法包括 至少一个允许预定物质与含LAM的样品共存的步骤; 用于测量含LAM的样品中的Et的方法和用于检测Et相关疾病的方法，其各自是使用鲎试剂测量内毒素的方法，该方法至少包括除去LAM与鲎的反应性的步骤 试剂通过上述去除方法; 用于测量含有LAM的样品中的BG的方法和用于检测真菌病的方法，其是使用鲎试剂测量BG的方法，所述方法至少包括通过所述除去的方法除去LAM与鲎试剂的反应性的步骤 方法; 等等。