公开(公告)号：US5641643A

公开(公告)日：1997-06-24

申请号：US542699

申请日：1995-10-13

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579 ,  C12Q1/37 ,  C08B37/18 ,  C12Q1/34

CPC分类号： C07H3/06 ,  C08B37/0024 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： 本发明涉及一种马蹄形褐飞虱细胞裂解物因子G活化抑制剂，其包含作为活性成分的含有至少一种由2至370（1-> 3）组成的聚（1-> 3）-β-D-葡萄糖苷结构部分的多糖苷 ）-β-D-葡萄糖苷结构单元，其结构彼此连续。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5474984A

公开(公告)日：1995-12-12

申请号：US246920

申请日：1994-05-20

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579 ,  A61K31/715 ,  C08B37/18

CPC分类号： C07H3/06 ,  C08B37/0024 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： 本发明涉及一种马蹄形褐飞虱细胞裂解物因子G活化抑制剂，其包含作为活性成分的含有至少一种由2至370（1-> 3）组成的聚（1-> 3）-β-D-葡萄糖苷结构部分的多糖苷 ）-β-D-葡萄糖苷结构单元，其结构彼此连续。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5155032A

公开(公告)日：1992-10-13

申请号：US474057

申请日：1990-05-01

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579

CPC分类号： C08B37/0024 ,  C07H3/06 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： PCT No.PCT / JP89 / 00903 Sec。 371日期1990年5月1日 102（e）日期1990年5月1日PCT提交1989年9月1日PCT公布。 公开号WO90 / 02951 日本3月22日，1990年。本发明涉及一种马蹄形蟹细胞裂解物因子G激活抑制剂，其包含含有至少一种由（2）〜（1→3）-β-D-葡萄糖苷结构部分组成的多糖苷的多糖苷作为活性成分 到具有连续结合的下式（IMAGE）的370（1-> 3）-β-D-葡萄糖苷结构单元。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US07851179B2

公开(公告)日：2010-12-14

申请号：US10584071

申请日：2004-12-22

申请人： Shigenori Tanaka ,  Shoji Takahashi

发明人： Shigenori Tanaka ,  Shoji Takahashi

IPC分类号： C12Q1/04

CPC分类号： G01N33/579 ,  G01N33/56911 ,  G01N2400/50

摘要： A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing a Limulus reagent to contact with a LAM-containing sample, a method for removing reactivity of LAM with a Limulus reagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using a Limulus reagent which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the described-described removing method; and the like.

摘要翻译： 一种用于测量LAM的方法和一种用于检测耐酸细菌的方法，其包括使鲎试剂与含LAM的样品接触的步骤，用于除去LAM与鲎试剂的反应性的方法，所述方法包括 至少一个允许预定物质与含LAM的样品共存的步骤; 用于测量含LAM的样品中的Et的方法和用于检测Et相关疾病的方法，其各自是使用鲎试剂测量内毒素的方法，该方法至少包括除去LAM与鲎的反应性的步骤 试剂通过上述去除方法; 用于测量含有LAM的样品中的BG的方法和用于检测真菌病的方法，其是使用鲎试剂测量BG的方法，所述方法至少包括通过所述除去的方法除去LAM与鲎试剂的反应性的步骤 方法; 等等。

公开(公告)号：US08703412B2

公开(公告)日：2014-04-22

申请号：US12905685

申请日：2010-10-15

申请人： Shigenori Tanaka ,  Shoji Takahashi

发明人： Shigenori Tanaka ,  Shoji Takahashi

IPC分类号： C12Q1/04 ,  C12Q1/06 ,  C12Q1/08 ,  G01N33/92

CPC分类号： G01N33/579 ,  G01N33/56911 ,  G01N2400/50

摘要： A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing a Limulus reagent to contact with a LAM-containing sample; a method for removing reactivity of LAM with a Limulus reagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the described-described removing method; and the like.

摘要翻译： 一种用于测量LAM的方法和一种用于检测耐酸细菌的方法，其至少包括允许鲎试剂与含LAM的样品接触的步骤; 用于除去LAM与鲎试剂的反应性的方法，该方法至少包括允许预定物质与含LAM的样品共存的步骤; 用于测量含LAM的样品中的Et的方法和用于检测Et相关疾病的方法，其各自是使用鲎试剂测量内毒素的方法，该方法至少包括除去LAM与鲎的反应性的步骤 试剂通过上述去除方法; 用于测量含有LAM的样品中的BG的方法和用于检测真菌病的方法，该方法是使用鲎试剂测量BG的方法，该方法至少包括通过上述描述的方法除去LAM与鲎试剂的反应性的步骤 去除方法; 等等。

公开(公告)号：US20110033939A1

公开(公告)日：2011-02-10

申请号：US12905685

申请日：2010-10-15

申请人： Shigenori Tanaka ,  Shoji Takahashi

发明人： Shigenori Tanaka ,  Shoji Takahashi

IPC分类号： G01N33/92

CPC分类号： G01N33/579 ,  G01N33/56911 ,  G01N2400/50

摘要： A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing a Limulus reagent to contact with a LAM-containing sample; a method for removing reactivity of LAM with a Limulus reagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the described-described removing method; and the like.

摘要翻译： 一种用于测量LAM的方法和一种用于检测耐酸细菌的方法，其至少包括允许鲎试剂与含LAM的样品接触的步骤; 用于除去LAM与鲎试剂的反应性的方法，该方法至少包括允许预定物质与含LAM的样品共存的步骤; 用于测量含LAM的样品中的Et的方法和用于检测Et相关疾病的方法，其各自是使用鲎试剂测量内毒素的方法，该方法至少包括除去LAM与鲎的反应性的步骤 试剂通过上述去除方法; 用于测量含有LAM的样品中的BG的方法和用于检测真菌病的方法，该方法是使用鲎试剂测量BG的方法，该方法至少包括通过上述描述的方法除去LAM与鲎试剂的反应性的步骤 去除方法; 等等。

公开(公告)号：US07638340B2

公开(公告)日：2009-12-29

申请号：US11993214

申请日：2006-06-21

申请人： Shigenori Tanaka ,  Shoji Takahashi

发明人： Shigenori Tanaka ,  Shoji Takahashi

IPC分类号： G01N33/92

CPC分类号： G01N33/579 ,  G01N2400/50 ,  Y10T436/25125

摘要： A method of eliminating the reactivity of lipoarabinomannan contained in a sample to a Limulus reagent including at least the step of allowing the sample containing lipoarabinomannan to coexist together with a metal salt or a buffer; and a method of assaying an endotoxin and a method of detecting an endotoxin-associated disease by using the above-described method.

摘要翻译： 一种消除样品中所含的阿拉伯糖甘露聚糖对鲎试剂的反应性的方法，包括至少包含使含有阿拉伯聚糖的样品与金属盐或缓冲液共存的步骤; 以及通过使用上述方法检测内毒素的方法和检测内毒素相关疾病的方法。

公开(公告)号：US20090117661A1

公开(公告)日：2009-05-07

申请号：US11993214

申请日：2006-06-21

申请人： Shigenori Tanaka ,  Shoji Takahashi

发明人： Shigenori Tanaka ,  Shoji Takahashi

IPC分类号： G01N33/92 ,  C07H15/06

CPC分类号： G01N33/579 ,  G01N2400/50 ,  Y10T436/25125

摘要： A method of eliminating the reactivity of lipoarabinomannan contained in a sample to a Limulus reagent including at least the step of allowing the sample containing lipoarabinomannan to coexist together with a metal salt or a buffer; and a method of assaying an endotoxin and a method of detecting an endotoxin-associated disease by using the above-described method.

摘要翻译： 一种消除样品中所含的阿拉伯糖甘露聚糖对鲎试剂的反应性的方法，包括至少包含使含有阿拉伯聚糖的样品与金属盐或缓冲液共存的步骤; 以及通过使用上述方法检测内毒素的方法和检测内毒素相关疾病的方法。

公开(公告)号：US20070154979A1

公开(公告)日：2007-07-05

申请号：US10584071

申请日：2004-12-22

申请人： Shigenori Tanaka ,  Shoji Takahashi

发明人： Shigenori Tanaka ,  Shoji Takahashi

IPC分类号： C12Q1/04

CPC分类号： G01N33/579 ,  G01N33/56911 ,  G01N2400/50

摘要： A method for measuring LAM and a method for detecting an acid-fast bacterium, which comprise at least a step of allowing a Limulus reagent to contact with a LAM-containing sample, a method for removing reactivity of LAM with a Limulus reagent, which comprises at least a step of allowing a predetermined substance to coexist with a LAM-containing sample; a method for measuring Et in a LAM-containing sample and a method for detecting an Et-related disease, which each is a method for measuring an endotoxin using a Limulus reagent, which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the above-described removing method; a method for measuring BG in a LAM-containing sample and a method for detecting mycosis, which is a method for measuring BG using a Limulus reagent which comprises at least a step of removing reactivity of LAM with a Limulus reagent by the described-described removing method; and the like.

摘要翻译： 一种用于测量LAM的方法和一种用于检测耐酸细菌的方法，其包括使鲎试剂与含LAM的样品接触的步骤，用于除去LAM与鲎试剂的反应性的方法，所述方法包括 至少一个允许预定物质与含LAM的样品共存的步骤; 用于测量含LAM的样品中的Et的方法和用于检测Et相关疾病的方法，其各自是使用鲎试剂测量内毒素的方法，该方法至少包括除去LAM与鲎的反应性的步骤 试剂通过上述去除方法; 用于测量含有LAM的样品中的BG的方法和用于检测真菌病的方法，其是使用鲎试剂测量BG的方法，所述方法至少包括通过所述除去的方法除去LAM与鲎试剂的反应性的步骤 方法; 等等。

公开(公告)号：US20090208995A1

公开(公告)日：2009-08-20

申请号：US12307890

申请日：2007-07-06

申请人： Hiroshi Tamura ,  Shoji Takahashi

发明人： Hiroshi Tamura ,  Shoji Takahashi

IPC分类号： C12Q1/26 ,  C12P21/06 ,  C12N9/00 ,  C12N7/00 ,  C12N5/06 ,  C07H21/04

CPC分类号： C12Q1/56 ,  C07K14/43509 ,  C12N9/6402 ,  C12N9/6408 ,  C12N2799/026 ,  G01N33/579 ,  Y02P20/582

摘要： Objects of the present invention are to provide a DNA fragment encoding a limulus-derived pro-clotting enzyme, a virus harboring the DNA fragment, a cell harboring the virus, a method of producing the pro-clotting enzyme by use of the cell, and means for assaying an endotoxin or (1→3)-β-D-glucan employing the enzyme, wherein these elements are capable of producing an endotoxin or (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily, at low cost, and on a large scale. In the present invention, for example, a DNA fragment encoding a protein having an amino acid sequence defined by SEQ ID NO: 4 is selected as a nucleic acid fragment encoding a limulus-derived pro-clotting enzyme, and the corresponding recombinant pro-clotting enzyme. Use of the enzyme can provide a high-sensitivity method and kit for detecting (1→3)-β-D-glucan and an endotoxin, utilizing a cascade reaction system in a horseshoe crab lysate.

摘要翻译： 本发明的目的是提供编码鲎来源的凝血酶的DNA片段，携带DNA片段的病毒，携带病毒的细胞，使用该细胞产生促凝酶的方法，以及 用于使用酶测定内毒素或（1-> 3）-β-D-葡聚糖的方法，其中这些元件能够产生令人满意质量的内毒素或（1-> 3）-β-D-葡聚糖测定试剂， 稳定地，以低成本，大规模。 在本发明中，例如，选择编码具有SEQ ID NO：4所定义的氨基酸序列的蛋白质的DNA片段作为编码鲎来源的凝血酶的核酸片段，将相应的重组凝血酶 酶。 使用酶可以提供用于检测（1→3）-β-D-葡聚糖和内毒素的高灵敏度方法和试剂盒，利用马蹄蟹溶解产物中的级联反应系统。