公开(公告)号：US5641643A

公开(公告)日：1997-06-24

申请号：US542699

申请日：1995-10-13

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579 ,  C12Q1/37 ,  C08B37/18 ,  C12Q1/34

CPC分类号： C07H3/06 ,  C08B37/0024 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： 本发明涉及一种马蹄形褐飞虱细胞裂解物因子G活化抑制剂，其包含作为活性成分的含有至少一种由2至370（1-> 3）组成的聚（1-> 3）-β-D-葡萄糖苷结构部分的多糖苷 ）-β-D-葡萄糖苷结构单元，其结构彼此连续。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5155032A

公开(公告)日：1992-10-13

申请号：US474057

申请日：1990-05-01

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579

CPC分类号： C08B37/0024 ,  C07H3/06 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： PCT No.PCT / JP89 / 00903 Sec。 371日期1990年5月1日 102（e）日期1990年5月1日PCT提交1989年9月1日PCT公布。 公开号WO90 / 02951 日本3月22日，1990年。本发明涉及一种马蹄形蟹细胞裂解物因子G激活抑制剂，其包含含有至少一种由（2）〜（1→3）-β-D-葡萄糖苷结构部分组成的多糖苷的多糖苷作为活性成分 到具有连续结合的下式（IMAGE）的370（1-> 3）-β-D-葡萄糖苷结构单元。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5474984A

公开(公告)日：1995-12-12

申请号：US246920

申请日：1994-05-20

申请人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

发明人： Shigenori Tanaka ,  Jun Aketagawa ,  Makoto Ohki ,  Shoji Takahashi ,  Hiroshi Tamura ,  Yuko Shibata

IPC分类号： C07H3/06 ,  C08B37/00 ,  G01N33/579 ,  A61K31/715 ,  C08B37/18

CPC分类号： C07H3/06 ,  C08B37/0024 ,  G01N33/579

摘要： This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1.fwdarw.3)-.beta.-D-glucoside structure portion consisting of 2 to 370 (1.fwdarw.3)-.beta.-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

摘要翻译： 本发明涉及一种马蹄形褐飞虱细胞裂解物因子G活化抑制剂，其包含作为活性成分的含有至少一种由2至370（1-> 3）组成的聚（1-> 3）-β-D-葡萄糖苷结构部分的多糖苷 ）-β-D-葡萄糖苷结构单元，其结构彼此连续。 该抑制剂可用于抑制鲎试验中使用的马蹄蟹血小板裂解物中可能存在的因子G的活化。

公开(公告)号：US5876955A

公开(公告)日：1999-03-02

申请号：US774236

申请日：1996-12-27

申请人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

发明人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

IPC分类号： G01N33/579 ,  C12Q1/34 ,  C12N9/99 ,  C12Q1/37

CPC分类号： G01N33/579

摘要： An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

摘要翻译： 提供了一种内毒素稳定剂，其可用于将试样或参考标准品的内毒素活性长时间维持在稳定状态，并制备具有减小的间隙变化的内毒素参照标准物，其对于 很长一段时间的形式，特别是一个解决方案，并承受反复使用。 还提供了包含上述内毒素稳定剂和内毒素的内毒素组合物和使用该内毒素测定内毒素的方法。 所述内毒素稳定剂包括：（1）选自氨基醇，多元醇，非离子表面活性剂，多糖和螯合剂中的至少一种物质; （2）至少氨基醇和多元醇; （3）多元醇优选为甘油或其衍生物; （4）除了组分（1）至（3）之外的聚乙二醇作为有效成分; 或（5）至少聚蔗糖，以及碱土金属盐和/或聚乙二醇作为有效成分。 内毒素组合物包含上述内毒素稳定剂。 用于测定内毒素的方法包括向样品中加入上述内毒素稳定剂，从而稳定样品中内毒素的活性。

公开(公告)号：US5648230A

公开(公告)日：1997-07-15

申请号：US474722

申请日：1995-06-07

申请人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

发明人： Hiroshi Tamura ,  Shigenori Tanaka ,  Jun Aketagawa ,  Toshio Oda

IPC分类号： G01N33/579 ,  C12Q1/34

CPC分类号： G01N33/579

摘要： An endotoxin stabilizing agent is provided, which is useful in order to maintain the endotoxin activity of a specimen or a reference standard in a stable state for a prolonged period of time and to prepare an endotoxin reference standard having a reduced intervial variation, being stable for a long time in the form of, in particular, a solution and withstanding repeated use. An endotoxin composition comprising the above-mentioned endotoxin stabilizing agent and endotoxin and a method for assaying endotoxin by using the same are also provided. The endotoxin stabilizing agent comprises: (1) at least one substance selected from the group consisting of aminoalcohols, polyhydric alcohols, nonionic surfactants, polysucroses and chelating agents; (2) at least aminoalcohol and polyhydric alcohol; (3) with the polyhydric alcohol being preferably glycerol or its derivative; (4) polyethylene glycol as an effective ingredient in addition to the components (1) to (3); or (5) at least polysucrose, and an alkaline earth metal salt and/or polyethylene glycol as an effective ingredient. The endotoxin composition comprises the above-identified endotoxin stabilizing agent. The method for assaying endotoxin comprises adding the above-described endotoxin stabilizing agent to a specimen to thereby stabilize the activity of endotoxin in the specimen.

摘要翻译： 提供了一种内毒素稳定剂，其可用于将试样或参考标准品的内毒素活性长时间维持在稳定状态，并制备具有减小的间隙变化的内毒素参照标准物，其对于 很长一段时间的形式，特别是一个解决方案，并承受反复使用。 还提供了包含上述内毒素稳定剂和内毒素的内毒素组合物和使用该内毒素测定内毒素的方法。 所述内毒素稳定剂包括：（1）选自氨基醇，多元醇，非离子表面活性剂，多糖和螯合剂中的至少一种物质; （2）至少氨基醇和多元醇; （3）多元醇优选为甘油或其衍生物; （4）除了组分（1）至（3）之外的聚乙二醇作为有效成分; 或（5）至少聚蔗糖，以及碱土金属盐和/或聚乙二醇作为有效成分。 内毒素组合物包含上述内毒素稳定剂。 用于测定内毒素的方法包括向样品中加入上述内毒素稳定剂，从而稳定样品中内毒素的活性。

公开(公告)号：US5681710A

公开(公告)日：1997-10-28

申请号：US391097

申请日：1995-02-21

申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

IPC分类号： G01N33/579 ,  C12Q1/56 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/44

CPC分类号： G01N33/579 ,  G01N2400/22 ,  G01N2400/24

摘要： The present invention provides a reagent for determining (1.fwdarw.3)-.beta.-D-glucan comprising a lysate substantially free from any endotoxin-sensitive factor, which is obtained by contacting limulus amebocyte lysate optionally containing dextran with a polyamide or cellulose insoluble carrier, which makes it possible to rapidly and easily determine at a high accuracy (1.fwdarw.3)-.beta.-D-glucan of mycotic origin contained in the body fluid such as blood or urine.

摘要翻译： 本发明提供了一种用于测定（1→3）-β-D-葡聚糖的试剂，其包含基本上不含任何内毒素敏感因子的裂解物，所述裂解物通过任选地含有葡聚糖的鲎变形细胞裂解物与聚酰胺或纤维素不溶性载体 ，这使得可以以高精度（1→3）-β-D-葡聚糖快速和容易地测定包含在体液如血液或尿液中的真菌来源。

公开(公告)号：US5550030A

公开(公告)日：1996-08-27

申请号：US120469

申请日：1993-09-14

申请人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

发明人： Shigenori Tanaka ,  Hiroshi Tamura ,  Makoto Ohki

IPC分类号： G03C5/50 ,  G01N33/545 ,  G01N33/548 ,  G01N33/579 ,  G03C7/407 ,  C12Q1/37 ,  C12N11/08 ,  C12N11/12 ,  C12Q1/34

CPC分类号： G01N33/579

摘要： Disclosed are a reagent for endotoxin-specific assay which comprises an insoluble carrier having immobilized thereon at least an endotoxin-sensitive factor derived from a limulus amebocyte; a kit for endotoxin-specific assay containing said reagent and a substrate for activated factor C or a substrate for clotting enzyme; a method for assaying endotoxin comprising applying a sample solution to said reagent to cause endotoxin in the sample to react with factor C in said reagent and determining a change of a substrate; and a process for preparing said reagent which comprises physically or chemically immobilizing at least an endotoxin-sensitive factor derived from a limulus amebocyte on an insoluble carrier. Endotoxin in a sample, even turbid or colored, can be specifically assayed with ease and rapidness without the influence of a (1.fwdarw.3)-.beta.-glucan.

摘要翻译： 公开了一种用于内毒素特异性测定的试剂，其包含其上固定有至少一种源自鲎变形细胞的内毒素敏感因子的不溶性载体; 含有所述试剂和活化因子C的底物或用于凝固酶的底物的内毒素特异性测定试剂盒; 一种用于测定内毒素的方法，包括将样品溶液施加到所述试剂以使样品中的内毒素与所述试剂中的因子C反应并确定底物的变化; 以及制备所述试剂的方法，其包括在不溶性载体上物理或化学地固定来自鲎变形细胞的至少一种内毒素敏感因子。 样品中的内毒素，甚至浑浊或有色，可以在不受（1-> 3）-β-葡聚糖的影响的情况下容易且快速地进行特异性测定。

公开(公告)号：US5389547A

公开(公告)日：1995-02-14

申请号：US58306

申请日：1993-05-10

申请人： Shigenori Tanaka ,  Hiroshi Tamura

发明人： Shigenori Tanaka ,  Hiroshi Tamura

IPC分类号： C12Q1/04 ,  C12Q1/37 ,  G01N33/531 ,  G01N33/579 ,  G01N33/00

CPC分类号： G01N33/579 ,  Y10T436/10 ,  Y10T436/14 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, .beta.-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulus reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.

摘要翻译： 试剂通过简单的处理变性或消除干扰生化反应的因素，而不需要分离任何变性产物沉淀物。 该试剂使得可以以高灵敏度快速有效地测定血液来源的样品中的β-葡聚糖和内毒素。 该试剂含有己二胺化合物和碱金属氢氧化物或碱金属氢氧化物作为主要成分。 还提供了一种使用试剂测定与鲎试剂特异性反应的物质的方法，至少包含试剂和鲎试剂的测定试剂，以及基于通过测定方法获得的结果诊断感染性疾病的方法。

公开(公告)号：US5378610A

公开(公告)日：1995-01-03

申请号：US859398

申请日：1992-05-28

申请人： Shigenori Tanaka ,  Hiroshi Tamura

发明人： Shigenori Tanaka ,  Hiroshi Tamura

IPC分类号： G01N33/579 ,  C12Q1/34 ,  C12Q1/00 ,  C12Q1/02

CPC分类号： G01N33/579

摘要： The present invention has been made in order to solve the problems in conventional methods of assaying an endotoxin in a specimen such as plasma or serum by the limulus test, which requires a complicated pretreatment procedure such as centrifugation for removing denatured precipitates formed with an acid treatment. According to the assay method of the present invention, an endotoxin adsorbed by proteins, lipids and platelets can be efficiently liberated simply by adding a mixed aqueous solution having a specific composition according to the present invention without any separation procedure and thus a sample solution of good qualities can be prepared. After adding the mixed aqueous solution containing a specific surfactant, a compound having an imidazolyl group or an amino group and an alkaline earth metal salt and an alkaline metal hydroxide to the specimen, followed by addition of the limulus amoebocyte lysate components, the endotoxin can be easily, quickly and precisely assayed. In particular, gram-negative bacterial septicemia, which can be hardly diagnosed, can be quickly assayed thereby.

摘要翻译： PCT No.PCT / JP91 / 01309 Sec。 371日期：1992年5月28日 102（e）日期1992年5月28日PCT 1991年9月27日PCT公布。 出版物WO92 / 06382 日期为1992年4月16日。本发明是为了解决通过鲎试验来测定血浆或血清等标本中的内毒素的常规方法中的问题，需要复杂的预处理步骤，例如离心分离 用酸处理形成变性沉淀。 根据本发明的测定方法，可以简单地通过添加根据本发明的具有特定组成的混合水溶液而无需任何分离程序，有效地释放被蛋白质，脂质和血小板吸附的内毒素，因此，可以有良好的样品溶液 品质可以做好准备。 将含有特定表面活性剂的混合水溶液，具有咪唑基或氨基的化合物和碱土金属盐和碱金属氢氧化物加入到样品中，然后加入鲎变形细胞裂解物组分，内毒素可以是 容易，快速和准确地测定。 特别地，可以快速测定可以很难诊断的革兰氏阴性细菌性败血症。

公开(公告)号：US06306577B1

公开(公告)日：2001-10-23

申请号：US09290091

申请日：1999-04-12

申请人： Hiroshi Tamura ,  Shigenori Tanaka

发明人： Hiroshi Tamura ,  Shigenori Tanaka

IPC分类号： C12Q100

CPC分类号： C12Q1/00 ,  G01N33/579 ,  G01N2333/90219 ,  G01N2333/90245 ,  Y02A90/26 ,  Y10S435/963

摘要： A method for measuring an enzyme reaction to determine an amount of a substance involved in the enzyme reaction, which comprises measuring a time course of a parameter of the enzyme reaction, measuring a time required for the parameter of the enzyme reaction to change from a first threshold value to a second threshold value, and correlating the measured time to an amount of the substance involved in the enzyme reaction.

摘要翻译： 一种测定酶反应以测定酶反应参与物质的量的方法，该方法包括测定酶反应参数的时间过程，测定酶反应参数所需的时间从第一 阈值至第二阈值，并且将测量的时间与酶反应中涉及的物质的量相关联。