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公开(公告)号：US20110113514A1
公开(公告)日：2011-05-12
申请号：US12909466
申请日：2010-10-21
IPC分类号： A01H5/10
CPC分类号： C12N15/8254
摘要： Disclosed herein are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase and a gene suppression molecule for lysine ketoglutarate reductase, the activity of which results in increased lysine in a plant seed.
摘要翻译： 本文公开了具有表达二氢吡啶二羧酸合酶的新型外源DNA构建体和赖氨酸酮戊二酸还原酶的基因抑制分子的转基因植物，其活性导致植物种子中赖氨酸增加。
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公开(公告)号：US09006414B2
公开(公告)日：2015-04-14
申请号：US12973821
申请日：2010-12-20
IPC分类号： C12N15/113 , C12N15/82 , C07H21/02 , C12N9/06 , C12N15/11
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N9/0028 , C12N15/111 , C12N15/8218 , C12N15/8251 , C12N15/8254 , C12N2310/11 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物，通过从重组DNA构建体转录，其以5'至3'的顺序包含可操作地连接到反义取向DNA元件和互补DNA元件的启动子元件。
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公开(公告)号：US08461418B2
公开(公告)日：2013-06-11
申请号：US12277196
申请日：2008-11-24
CPC分类号： A01H5/10 , A23K10/30 , A23L7/198 , C07K14/415 , C12N9/0028 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8254
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物通过从包含5'至3'顺序的重组DNA构建体转录起可操作地连接到多于一个反义取向DNA元件和一个或多个互补DNA元件的启动子元件。
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公开(公告)号：US20110126321A1
公开(公告)日：2011-05-26
申请号：US12973821
申请日：2010-12-20
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N9/0028 , C12N15/111 , C12N15/8218 , C12N15/8251 , C12N15/8254 , C12N2310/11 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物，通过从重组DNA构建体转录，其以5'至3'的顺序包含可操作地连接到反义取向DNA元件和互补DNA元件的启动子元件。
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公开(公告)号：US07855323B2
公开(公告)日：2010-12-21
申请号：US11057062
申请日：2005-02-10
IPC分类号： C12N15/82
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N9/0028 , C12N15/111 , C12N15/8218 , C12N15/8251 , C12N15/8254 , C12N2310/11 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物，通过重组DNA构建体的转录，其以5'至3'的顺序包含可操作地连接到反义取向DNA元件和互补DNA元件的启动子元件。
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公开(公告)号：US20090158463A1
公开(公告)日：2009-06-18
申请号：US12277196
申请日：2008-11-24
CPC分类号： A01H5/10 , A23K10/30 , A23L7/198 , C07K14/415 , C12N9/0028 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8254
摘要： Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
摘要翻译： 在转基因生物体的细胞中产生以反义取向RNA环形式的反义RNA RNA基因抑制剂。 植物通过从包含5'至3'顺序的重组DNA构建体转录起可操作地连接到多于一个反义取向DNA元件和一个或多个互补DNA元件的启动子元件。
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公开(公告)号：US20070192896A1
公开(公告)日：2007-08-16
申请号：US11513543
申请日：2006-08-31
CPC分类号： C12N15/8254 , C12N15/8218
摘要： Transgenic corn seed with high levels of free lysine resulting from a recombinant DNA construct having an endosperm specific promoter and an embryo specific promoter operably linked to gene suppression elements for suppressing the production of an endogenous lysine catabolite.
摘要翻译： 由具有胚乳特异性启动子的重组DNA构建体产生的具有高水平游离赖氨酸的转基因玉米种子和与抑制内源赖氨酸分解代谢产物产生的基因抑制元件可操作地连接的胚胎特异性启动子。
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公开(公告)号：US09000264B2
公开(公告)日：2015-04-07
申请号：US12832942
申请日：2010-07-08
申请人： Edwards M. Allen , Larry A. Gilbertson , Nancy M. Houmard , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
发明人： Edwards M. Allen , Larry A. Gilbertson , Nancy M. Houmard , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US09212370B2
公开(公告)日：2015-12-15
申请号：US12726673
申请日：2010-03-18
申请人： Edwards M. Allen , Larry A. Gilbertson , Sara E. Heisel , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
发明人： Edwards M. Allen , Larry A. Gilbertson , Sara E. Heisel , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00 , C12N15/113
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US07982105B2
公开(公告)日：2011-07-19
申请号：US11513543
申请日：2006-08-31
CPC分类号： C12N15/8254 , C12N15/8218
摘要： Transgenic corn seed with high levels of free lysine resulting from a recombinant DNA construct having an endosperm specific promoter and an embryo specific promoter operably linked to gene suppression elements for suppressing the production of an endogenous lysine catabolite.
摘要翻译： 由具有胚乳特异性启动子的重组DNA构建体产生的具有高水平游离赖氨酸的转基因玉米种子和与抑制内源赖氨酸分解代谢产物产生的基因抑制元件可操作地连接的胚胎特异性启动子。
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