公开(公告)号：US08609346B2

公开(公告)日：2013-12-17

申请号：US11920167

申请日：2006-05-11

申请人： Shinobu Kitazume ,  Ritsuko Oka ,  Yasuhiro Hashimoto ,  Tamao Endo ,  Makoto Higuchi ,  Hiroyuki Arai ,  Takaomi Saido ,  Keiko Sato

发明人： Shinobu Kitazume ,  Ritsuko Oka ,  Yasuhiro Hashimoto ,  Yuji Sato ,  Tamao Endo ,  Makoto Higuchi ,  Hiroyuki Arai ,  Takaomi Saido

IPC分类号： G01N33/53 ,  G01N33/96

CPC分类号： G01N33/6896 ,  G01N2800/2821

摘要： It is an object of the present invention to detect a change in the metabolism of a glycoprotein having an α2,6-sialyl residue, which is contained in blood, so as to provide an agent and a method for diagnosing sporadic Alzheimer's disease. The present invention provides an agent for diagnosing Alzheimer's disease, which comprises lectin used for detecting an α2,6-sialyl residue-containing glycoprotein.

摘要翻译： 本发明的目的是检测含有血液中的具有α2,6-唾液酸残基的糖蛋白的代谢变化，从而提供用于诊断散发性阿尔茨海默病的药剂和方法。 本发明提供了用于诊断阿尔茨海默氏病的药剂，其包含用于检测含有α2,6-唾液酸残基的糖蛋白的凝集素。

公开(公告)号：US20110076704A1

公开(公告)日：2011-03-31

申请号：US11920167

申请日：2006-05-11

申请人： Shinobu Kitazume ,  Ritsuko Oka ,  Yasuhiro Hashimoto ,  Yuji Sato ,  Tamao Endo ,  Makoto Higuchi ,  Hiroyuki Arai ,  Takaomi Saido ,  Keiko Sato

发明人： Shinobu Kitazume ,  Ritsuko Oka ,  Yasuhiro Hashimoto ,  Yuji Sato ,  Tamao Endo ,  Makoto Higuchi ,  Hiroyuki Arai ,  Takaomi Saido ,  Keiko Sato

IPC分类号： G01N33/53 ,  C07K14/42 ,  C07K16/18

CPC分类号： G01N33/6896 ,  G01N2800/2821

摘要： It is an object of the present invention to detect a change in the metabolism of a glycoprotein having an α2,6-sialyl residue, which is contained in blood, so as to provide an agent and a method for diagnosing sporadic Alzheimer's disease. The present invention provides an agent for diagnosing Alzheimer's disease, which comprises lectin used for detecting an α2,6-sialyl residue-containing glycoprotein.

摘要翻译： 本发明的目的是检测血液中含有的具有α2,6-唾液酰残基的糖蛋白的代谢变化，从而提供用于诊断散发性阿尔茨海默病的药剂和方法。 本发明提供了用于诊断阿尔茨海默病的药剂，其包含用于检测含有α2,6-唾液酸残基的糖蛋白的凝集素。

公开(公告)号：US20130203184A1

公开(公告)日：2013-08-08

申请号：US13813073

申请日：2011-07-29

申请人： Shinobu Kitazume ,  Naoyuki Taniguchi ,  Yuriko Tachida ,  Takaomi Saido ,  Yasuhiro Hashimoto ,  Takashi Honda

发明人： Shinobu Kitazume ,  Naoyuki Taniguchi ,  Yuriko Tachida ,  Takaomi Saido ,  Yasuhiro Hashimoto ,  Takashi Honda

IPC分类号： G01N33/68

CPC分类号： G01N33/6896 ,  G01N2333/4709 ,  G01N2800/2821

摘要： The present invention provides a method of detecting soluble amyloid β precursor protein 770β derived from vascular endothelial cell, comprising the following steps: (1) a step of contacting a biological sample derived from a test subject suspected of having a disease accompanied by accumulation of amyloid β peptide with an antibody recognizing soluble amyloid β precursor protein β or an amyloid β precursor protein 770-specific antibody; and (2) a step of detecting soluble amyloid β precursor protein 770β in the complex formed in step (1).

摘要翻译： 本发明提供一种检测来自血管内皮细胞的可溶性淀粉样蛋白β前体蛋白770beta的方法，其包括以下步骤：（1）使来自疑似患有伴有淀粉样蛋白积累的疾病的受检对象的生物样品接触的步骤 具有识别可溶性淀粉样蛋白β前体蛋白β或淀粉样蛋白β前体蛋白770特异性抗体的抗体的β肽; 和（2）在步骤（1）中形成的络合物中检测可溶性淀粉样蛋白β前体蛋白770beta的步骤。

公开(公告)号：US20130034858A1

公开(公告)日：2013-02-07

申请号：US13582403

申请日：2011-03-03

申请人： Haruhisa Inoue ,  Shiho Kitaoka ,  Naoki Yahata ,  Nobuhisa Iwata ,  Takaomi Saido

发明人： Haruhisa Inoue ,  Shiho Kitaoka ,  Naoki Yahata ,  Nobuhisa Iwata ,  Takaomi Saido

IPC分类号： C12Q1/37 ,  G01N21/64 ,  C12Q1/68 ,  C12Q1/02

CPC分类号： G01N33/6896 ,  C12N5/0619 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/91 ,  C12N2503/02 ,  C12N2506/00 ,  C12N2506/45 ,  C12N2533/32 ,  G01N2333/4709 ,  G01N2333/96486 ,  G01N2800/2821

摘要： The present invention provides a method for detecting onset of, or the risk of development of, a protein misfolding disease, and a method for predicting the age of onset of a protein misfolding disease using nerve cells derived from iPS cells. The present invention also provides a kit to be used in these methods.

摘要翻译： 本发明提供了一种用于检测蛋白质错折叠疾病的发病或发展的风险的方法，以及使用源自iPS细胞的神经细胞来预测蛋白质错折叠疾病发病年龄的方法。 本发明还提供了用于这些方法的试剂盒。

公开(公告)号：US20090298775A1

公开(公告)日：2009-12-03

申请号：US12491120

申请日：2009-06-24

申请人： Takaomi Saido ,  Nobuhisa Iwata ,  Satoshi Tsubuki ,  Yoshie Takaki ,  Takashi Saito ,  Tadashi Nakaya

发明人： Takaomi Saido ,  Nobuhisa Iwata ,  Satoshi Tsubuki ,  Yoshie Takaki ,  Takashi Saito ,  Tadashi Nakaya

IPC分类号： A61K38/31 ,  A61K38/10 ,  A61K38/12

CPC分类号： C12Q1/37 ,  G01N33/6896 ,  G01N2333/96486 ,  G01N2500/00

摘要： The present invention provides a method of measuring the activity of neprilysin, etc. More specifically, the present invention provides a method of measuring the activity of neprilysin in nerve cells; a method of screening a protein, a peptide or a compound enhancing the activity or expression of neprilysin in nerve cells by measuring the activity of neprilysin; a method of enhancing the activity or expression of neprilysin; and so on. Thus, the compound enhancing the activity and/or expression of neprilysin, which is obtained by the screening method characterized by using the method of measuring the activity of neprilysin in accordance with the present invention, is useful as a preventive and/or therapeutic agent for Alzheimer's disease. The method of measuring the activity of the present invention can be used for presymptomatic diagnosis of Alzheimer's disease.

摘要翻译： 本发明提供了测定尿丙氨酸等的活性的方法。更具体地，本发明提供了测定神经细胞中尿丙氨酸蛋白酶活性的方法。 通过测量尿丙氨酸的活性来筛选蛋白质，肽或增强神经细胞中neprilysin的活性或表达的化合物的方法; 增强头孢氨苄的活性或表达的方法; 等等。 因此，通过使用根据本发明的测定尿丙氨酸的活性的方法的筛选方法获得的增强头孢氨苄的活性和/或表达的化合物可用作预防和/或治疗剂 阿尔茨海默氏病。 测定本发明活性的方法可用于阿尔茨海默病的症状诊断。

公开(公告)号：US09097727B2

公开(公告)日：2015-08-04

申请号：US13582403

申请日：2011-03-03

申请人： Haruhisa Inoue ,  Shiho Kitaoka ,  Naoki Yahata ,  Nobuhisa Iwata ,  Takaomi Saido

发明人： Haruhisa Inoue ,  Shiho Kitaoka ,  Naoki Yahata ,  Nobuhisa Iwata ,  Takaomi Saido

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/573 ,  G01N33/68 ,  C12N5/0793

CPC分类号： G01N33/6896 ,  C12N5/0619 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/91 ,  C12N2503/02 ,  C12N2506/00 ,  C12N2506/45 ,  C12N2533/32 ,  G01N2333/4709 ,  G01N2333/96486 ,  G01N2800/2821

摘要： The present invention provides a method for detecting onset of, or the risk of development of, a protein misfolding disease, and a method for predicting the age of onset of a protein misfolding disease using nerve cells derived from iPS cells. The present invention also provides a kit to be used in these methods.

摘要翻译： 本发明提供了一种用于检测蛋白质错折叠疾病的发病或发展的风险的方法，以及使用源自iPS细胞的神经细胞来预测蛋白质错折叠疾病发病年龄的方法。 本发明还提供了用于这些方法的试剂盒。

公开(公告)号：US07572574B2

公开(公告)日：2009-08-11

申请号：US10512588

申请日：2003-04-24

申请人： Takaomi Saido ,  Nobuhisa Iwata ,  Satoshi Tsubuki ,  Yoshie Takaki ,  Takashi Saito ,  Tadashi Nakaya

发明人： Takaomi Saido ,  Nobuhisa Iwata ,  Satoshi Tsubuki ,  Yoshie Takaki ,  Takashi Saito ,  Tadashi Nakaya

IPC分类号： C12Q1/00 ,  G01N33/53 ,  G01N33/567 ,  G01N33/542 ,  G01N33/537

CPC分类号： C12Q1/37 ,  G01N33/6896 ,  G01N2333/96486 ,  G01N2500/00

摘要： The present invention provides a method of measuring the activity of neprilysin, etc. More specifically, the present invention provides a method of measuring the activity of neprilysin in nerve cells; a method of screening a protein, a peptide or a compound enhancing the activity or expression of neprilysin nerve cells by measuring the activity of neprilysin; a method of enhancing the activity or expression of neprilysin; and so on. Thus, the compound enhancing the activity and/or expression of neprilysin, which is obtained by the screening method characterized by using the method of measuring the activity of neprilysin in accordance with the present invention, is useful as a preventive and/or therapeutic agent for Alzheimer's disease. The method of measuring the activity of the present invention can be used for presymptoms diagnosis of Alzheimer's disease.

摘要翻译： 本发明提供了测定尿丙氨酸等的活性的方法。更具体地，本发明提供了测定神经细胞中尿丙氨酸蛋白酶活性的方法。 通过测定尿丙氨酸的活性来筛选蛋白质，肽或增强neprilysin神经细胞活性或表达的化合物的方法; 增强头孢氨苄的活性或表达的方法; 等等。 因此，通过使用根据本发明的测定尿丙氨酸的活性的方法的筛选方法获得的增强头孢氨苄的活性和/或表达的化合物可用作预防和/或治疗剂 阿尔茨海默氏病。 测定本发明的活性的方法可用于阿尔茨海默病的症状诊断。

公开(公告)号：US07745688B2

公开(公告)日：2010-06-29

申请号：US11765730

申请日：2007-06-20

申请人： Takaomi Saido ,  Nobuhisa Iwata ,  Takashi Saito ,  Takahiro Suemoto ,  Jiro Takano

发明人： Takaomi Saido ,  Nobuhisa Iwata ,  Takashi Saito ,  Takahiro Suemoto ,  Jiro Takano

IPC分类号： A01K67/033 ,  G01N33/00

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2227/106 ,  A01K2267/0312

摘要： The present invention provides a non-human model mammal of Alzheimer's disease (AD) containing chimeric amyloid precursor protein (APP) gene capable of producing human amyloid β peptide (Aβ) or a living part thereof, characterized in that Aβ42/Aβ40 ratio at 8-weeks-old is about 7-fold or more (about 140-fold or more in homozygote) higher compared to that of a corresponding wild-type mammal. Moreover, the present invention provides the mammal or a living part thereof, further characterized in that the level of APP expression is not significantly different compared to the corresponding wild-type mammal, and a screening method for a prophylactic and/or therapeutic drug for AD, a biomarker in biological fluids and molecular imaging of amyloid deposition or other pathological changes in the brain for an early diagnosis of AD using the mammal or a living part thereof.

摘要翻译： 本发明提供了含有能够产生人淀粉样蛋白的嵌合淀粉样蛋白前体蛋白（APP）基因的非人模型的阿尔茨海默病（AD）哺乳动物。 肽（A＆bgr）或其活性部分，其特征在于8周龄的A / B比值与约7倍或更多（纯合子中约140倍或更多）相比较， 相应的野生型哺乳动物。 此外，本发明提供了哺乳动物或其生物体部分，其特征还在于，与相应的野生型哺乳动物相比，APP表达水平没有显着差异，以及用于AD的预防和/或治疗药物的筛选方法 生物流体中的生物标志物和淀粉样蛋白沉积的分子成像或脑中的其它病理变化，以便使用哺乳动物或其生物部分进行AD的早期诊断。

公开(公告)号：US07122374B1

公开(公告)日：2006-10-17

申请号：US10406980

申请日：2003-04-04

申请人： Takaomi Saido ,  Takashi Kuda

发明人： Takaomi Saido ,  Takashi Kuda

IPC分类号： C12N5/20 ,  C07K16/18 ,  C12P21/08 ,  A61K39/395

CPC分类号： C07K16/18

摘要： AβN3pE-42 is a β amyloid protein that accumulates specifically as a major constituent of senile plaque in the brains of both sporadic and familial Alzheimer's disease patients. The invention provides antibodies that specifically recognize AβN3pE-42 and can be expected to have a strong β amyloid-removing action. Particularly, humanized antibodies against AβN3pE-42 are useful to treat human neurodegenerative diseases. Further, since AβN3pE-42 is localized in the brain, the antibodies of the invention can avoid side effects such as kidney disorders caused by the formation of antigen-antibody complex in the blood. An agent for gene therapy using a vector in which a cDNA encoding a protein comprises the antigen-binding region of the antibody can be an efficient therapeutic drug for removing β amyloid from the brain.

摘要翻译： Abeta N3pE-42是一种β淀粉样蛋白，其在散发性和家族性阿尔茨海默病患者的大脑中特异性地积聚作为老年斑的主要成分。 本发明提供了特异性识别Abeta N3pE-42的抗体，并且可以预期具有强的β淀粉样蛋白去除作用。 特别地，针对Abeta N3pE-42的人源化抗体可用于治疗人神经变性疾病。 此外，由于Abeta N3pE-42位于脑中，所以本发明的抗体可以避免副作用，例如血液中形成抗原 - 抗体复合物引起的肾脏疾病。 使用其中编码蛋白质的cDNA包含抗体的抗原结合区域的载体的基因治疗剂可以是从脑中去除β淀粉样蛋白的有效治疗药物。

公开(公告)号：US20060008842A1

公开(公告)日：2006-01-12

申请号：US10512588

申请日：2003-04-24

申请人： Takaomi Saido ,  Nobuhisa Iwata ,  Satoshi Tsubuki ,  Yoshie Takaki ,  Takashi Saito

发明人： Takaomi Saido ,  Nobuhisa Iwata ,  Satoshi Tsubuki ,  Yoshie Takaki ,  Takashi Saito

IPC分类号： G01N33/53

CPC分类号： C12Q1/37 ,  G01N33/6896 ,  G01N2333/96486 ,  G01N2500/00

摘要： The present invention provides a method of measuring the activity of neprilysin, etc. More specifically, the present invention provides a method of measuring the activity of neprilysin in nerve cells; a method of screening a protein, a peptide or a compound enhancing the activity or expression of neprilysin nerve cells by measuring the activity of neprilysin; a method of enhancing the activity or expression of neprilysin; and so on. Thus, the compound enhancing the activity and/or expression of neprilysin, which is obtained by the screening method characterized by using the method of measuring the activity of neprilysin in accordance with the present invention, is useful as a preventive and/or therapeutic agent for Alzheimer's disease. The method of measuring the activity of the present invention can be used for presymptoms diagnosis of Alzheimer's disease.