公开(公告)号：US10370706B1

公开(公告)日：2019-08-06

申请号：US16111590

申请日：2018-08-24

申请人： Shuichi Hoshika ,  Nicole A Leal ,  Steven A Benner

发明人： Shuichi Hoshika ,  Nicole A Leal ,  Steven A Benner

IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12P19/34 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q1/686

摘要： This invention covers a new molecular recognition system, where duplexes of DNA-like molecules comprise segments built from nucleotides that carry only a small pyrimidine-like analog, and where the segments pair by pyrimidine analog:pyrimidine analog “skinny” pairing. This pairing retains hydrogen bonding complementarity. Further, this invention relates to processes for preparing those duplexes, and processes that use such duplexes as primer:template complexes for reactions catalyzed by DNA polymerases.

公开(公告)号：US20210238643A1

公开(公告)日：2021-08-05

申请号：US17188248

申请日：2021-03-01

申请人： Nicole A Leal ,  Steven A Benner

发明人： Nicole A Leal ,  Steven A Benner

IPC分类号： C12P19/34 ,  C07H21/02

摘要： This invention relates to nucleotide analogs and their derivatives (termed non-standard nucleotides) that, when incorporated into DNA and RNA, expand the number of nucleotides beyond the four found in standard DNA and RNA. The invention further relates to enzymatic processes that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives. The RNA polymerases of the instant invention transcribe DNA containing nonstandard nucleotides to give RNA containing nonstandard nucleotides, where certain of those nucleotides have nucleobases that do not present electron density to the minor groove.

公开(公告)号：US10934569B1

公开(公告)日：2021-03-02

申请号：US16226963

申请日：2018-12-20

申请人： Nicole A Leal ,  Steven A Benner

发明人： Nicole A Leal ,  Steven A Benner

IPC分类号： C12P19/34

摘要： This invention relates to processes that transcribe DNA molecules containing non-standard nucleotides using variants of T7 RNA polymerase to give RNA transcripts that contain their complementary non-standard nucleotides. Non-standard nucleotides pair during transcription using patterns of hydrogen bonding that are different from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.

公开(公告)号：US10654841B1

公开(公告)日：2020-05-19

申请号：US15786086

申请日：2017-10-17

申请人： Steven A Benner ,  Nicole A Leal

发明人： Steven A Benner ,  Nicole A Leal

IPC分类号： C07D413/14 ,  C12P19/34 ,  C07H21/04

摘要： This invention claims processes that comprise the appending of nucleotides having a 3′-ONH2 moiety to the 3′-ends of oligonucleotide primers using 3′-deoxynucleoside triphosphates of 2′-deoxynucleoside derivatives that have, instead of a 3′-OH moiety, a 3′-ONH2 moiety, where the nucleotides contain non-standard nucleobases.

公开(公告)号：US11117922B1

公开(公告)日：2021-09-14

申请号：US16679501

申请日：2019-11-11

申请人： Nicole A Leal ,  Steven A Benner

发明人： Nicole A Leal ,  Steven A Benner

IPC分类号： C07H21/04 ,  C12N15/66 ,  C12N15/10 ,  C07H19/20 ,  C07H19/10 ,  C07H19/12 ,  C07H19/14 ,  C07H19/048

摘要： This invention claims processes that append a single nucleotide having a 3′-ONH2 moiety to the 3′-ends of an oligonucleotide primer using 3′-deoxynucleoside triphosphates that have, instead of a 3′-OH moiety, a 3′-ONH2 moiety, where the nucleotides contain both standard and non-standard nucleobases, and where as a key claim limitation, substantially no hydroxylamine is present in the solutions used in the claimed processes.

公开(公告)号：US10829812B1

公开(公告)日：2020-11-10

申请号：US16216266

申请日：2018-12-11

申请人： Steven A Benner ,  Roberto Laos ,  Nicole A Leal ,  Zunyi Yang ,  Myong Jung Kim

发明人： Steven A Benner ,  Roberto Laos ,  Nicole A Leal ,  Zunyi Yang ,  Myong Jung Kim

IPC分类号： C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6806

摘要： This invention relates to processes that amplify oligonucleotide analogs that incorporate non-standard nucleobase analogs from an artificially expanded genetic information system. These pair in DNA duplexes via patterns of hydrogen bonds that differ from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.