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25 条记录 (耗时 0.016 秒)

    Enzymatic Processes for Synthesizing RNA Containing Certain Non-Standard Nucleotides
    1.
    发明申请
    Enzymatic Processes for Synthesizing RNA Containing Certain Non-Standard Nucleotides 有权

    公开(公告)号：US20210238643A1

    公开(公告)日：2021-08-05

    申请号：US17188248

    申请日：2021-03-01

    申请人： Nicole A Leal ,  Steven A Benner

    发明人： Nicole A Leal ,  Steven A Benner

    IPC分类号： C12P19/34 ,  C07H21/02

    摘要： This invention relates to nucleotide analogs and their derivatives (termed non-standard nucleotides) that, when incorporated into DNA and RNA, expand the number of nucleotides beyond the four found in standard DNA and RNA. The invention further relates to enzymatic processes that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives. The RNA polymerases of the instant invention transcribe DNA containing nonstandard nucleotides to give RNA containing nonstandard nucleotides, where certain of those nucleotides have nucleobases that do not present electron density to the minor groove.

    Nucleoside triphosphates with stable aminoxy groups
    8.
    发明授权
    Nucleoside triphosphates with stable aminoxy groups 有权

    公开(公告)号：US10472383B2

    公开(公告)日：2019-11-12

    申请号：US15460475

    申请日：2017-03-16

    申请人： Steven A Benner

    发明人： Steven A Benner

    IPC分类号： C07H19/04 ,  C07H19/20 ,  C07H19/10

    摘要： This invention claims aqueous compositions that comprise triphosphates of 2′-deoxynucleoside derivatives that have, instead of a 3′-OH moiety, a 3′-ONH2 moiety; wherein said compositions contain less than 0.5 mole percent contaminating triphosphate having a 3′-OH moiety, as well as processes for providing such compositions. The compositions further must contain insubstantial amounts of hydroxylamine.

    Processes for point of care detection of DNA and RNA
    9.
    发明授权
    Processes for point of care detection of DNA and RNA 有权

    公开(公告)号：US10106837B1

    公开(公告)日：2018-10-23

    申请号：US14613960

    申请日：2015-02-04

    申请人： Steven A Benner

    发明人： Steven A Benner

    IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6844

    摘要： This invention provides a single process that collects any and all target nucleic acids from a sample that can contain any of a number of biological species, including bacteria, viruses, or fungi, where the first step renders the sample nonhazardous and releases nucleic acids from cell structures, compartments, and molecular complexes that may hold them, the second step concentrates the target nucleic acids from even very highly dilute states, in the third step releases these in a form that, following dilution, makes them compatible with processes that amplify nucleic acids, both standard PCR as well as isothermal amplification protocols. The pre-amplification process involves ammonia solutions no more toxic than those found in the commercial product Windex, requires no electrical power, requires materials no more complex than water desalinization resin and salt, and can be used by inexperienced individuals.

    Molecular recognition systems with pyrimidine analog pairing
    10.
    发明授权
    Molecular recognition systems with pyrimidine analog pairing 有权

    公开(公告)号：US10059737B1

    公开(公告)日：2018-08-28

    申请号：US15461073

    申请日：2017-03-16

    申请人： Steven A Benner ,  Shuichi Hoshika

    发明人： Steven A Benner ,  Shuichi Hoshika

    IPC分类号： C07H21/04

    CPC分类号： C07H21/04

    摘要： This invention is for a new molecular recognition system, where DNA-like molecules comprising segments built from nucleotides that carry only a small which comprises process by which one molecule interacts with a specific second molecule, or by which a portion of a single molecule interacts specifically with another portion of the same molecule. Further, this invention relates to molecular recognition that follows simple rules. Further, this invention relates to molecular systems that are linear biopolymers that are analogs of DNA and RNA, in that they are built from a small set of building blocks that are linked together by phosphate groups, where the building blocks comprise a sugar (ribose, 2′-deoxyribose, or an analog) attached to a heterocycle. The molecular recognition that they display differs from that displayed by DNA and RNA, in that the rules governing molecular recognition break the rules of size complementarity followed in molecular recognition displayed between and within strands of DNA and RNA.