公开(公告)号：US20210238643A1

公开(公告)日：2021-08-05

申请号：US17188248

申请日：2021-03-01

申请人： Nicole A Leal ,  Steven A Benner

发明人： Nicole A Leal ,  Steven A Benner

IPC分类号： C12P19/34 ,  C07H21/02

摘要： This invention relates to nucleotide analogs and their derivatives (termed non-standard nucleotides) that, when incorporated into DNA and RNA, expand the number of nucleotides beyond the four found in standard DNA and RNA. The invention further relates to enzymatic processes that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives. The RNA polymerases of the instant invention transcribe DNA containing nonstandard nucleotides to give RNA containing nonstandard nucleotides, where certain of those nucleotides have nucleobases that do not present electron density to the minor groove.

公开(公告)号：US11117922B1

公开(公告)日：2021-09-14

申请号：US16679501

申请日：2019-11-11

申请人： Nicole A Leal ,  Steven A Benner

发明人： Nicole A Leal ,  Steven A Benner

IPC分类号： C07H21/04 ,  C12N15/66 ,  C12N15/10 ,  C07H19/20 ,  C07H19/10 ,  C07H19/12 ,  C07H19/14 ,  C07H19/048

摘要： This invention claims processes that append a single nucleotide having a 3′-ONH2 moiety to the 3′-ends of an oligonucleotide primer using 3′-deoxynucleoside triphosphates that have, instead of a 3′-OH moiety, a 3′-ONH2 moiety, where the nucleotides contain both standard and non-standard nucleobases, and where as a key claim limitation, substantially no hydroxylamine is present in the solutions used in the claimed processes.

公开(公告)号：US10829812B1

公开(公告)日：2020-11-10

申请号：US16216266

申请日：2018-12-11

申请人： Steven A Benner ,  Roberto Laos ,  Nicole A Leal ,  Zunyi Yang ,  Myong Jung Kim

发明人： Steven A Benner ,  Roberto Laos ,  Nicole A Leal ,  Zunyi Yang ,  Myong Jung Kim

IPC分类号： C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6806

摘要： This invention relates to processes that amplify oligonucleotide analogs that incorporate non-standard nucleobase analogs from an artificially expanded genetic information system. These pair in DNA duplexes via patterns of hydrogen bonds that differ from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.

公开(公告)号：US10370706B1

公开(公告)日：2019-08-06

申请号：US16111590

申请日：2018-08-24

申请人： Shuichi Hoshika ,  Nicole A Leal ,  Steven A Benner

发明人： Shuichi Hoshika ,  Nicole A Leal ,  Steven A Benner

IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12P19/34 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q1/686

摘要： This invention covers a new molecular recognition system, where duplexes of DNA-like molecules comprise segments built from nucleotides that carry only a small pyrimidine-like analog, and where the segments pair by pyrimidine analog:pyrimidine analog “skinny” pairing. This pairing retains hydrogen bonding complementarity. Further, this invention relates to processes for preparing those duplexes, and processes that use such duplexes as primer:template complexes for reactions catalyzed by DNA polymerases.

公开(公告)号：US10934569B1

公开(公告)日：2021-03-02

申请号：US16226963

申请日：2018-12-20

申请人： Nicole A Leal ,  Steven A Benner

发明人： Nicole A Leal ,  Steven A Benner

IPC分类号： C12P19/34

摘要： This invention relates to processes that transcribe DNA molecules containing non-standard nucleotides using variants of T7 RNA polymerase to give RNA transcripts that contain their complementary non-standard nucleotides. Non-standard nucleotides pair during transcription using patterns of hydrogen bonding that are different from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.

公开(公告)号：US10654841B1

公开(公告)日：2020-05-19

申请号：US15786086

申请日：2017-10-17

申请人： Steven A Benner ,  Nicole A Leal

发明人： Steven A Benner ,  Nicole A Leal

IPC分类号： C07D413/14 ,  C12P19/34 ,  C07H21/04

摘要： This invention claims processes that comprise the appending of nucleotides having a 3′-ONH2 moiety to the 3′-ends of oligonucleotide primers using 3′-deoxynucleoside triphosphates of 2′-deoxynucleoside derivatives that have, instead of a 3′-OH moiety, a 3′-ONH2 moiety, where the nucleotides contain non-standard nucleobases.

公开(公告)号：US20110117554A1

公开(公告)日：2011-05-19

申请号：US12927430

申请日：2010-11-15

申请人： Steven Albert Benner ,  Daniel Hutter ,  Nicole A. Leal

发明人： Steven Albert Benner ,  Daniel Hutter ,  Nicole A. Leal

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q2525/113 ,  C12Q2527/125

摘要： Specific sequences of DNA are often detected by a process that comprises a step where the sequence to be detected (the “analyte”) binds to give a duplex with a DNA molecule or analog that is complementary in the Watson-Crick sense to some portion of the analyte in an aqueous “assay environment” that may contain buffer, salt, and/or detergent. Such purely aqueous systems cannot be exposed indefinitely to the environment, however, as the water in the system will evaporate. Further, such systems often support the growth of bacteria and other organisms, destroying their effectiveness. This invention provides for compositions of matter and processes that use them that comprise assay mixtures containing more than 40% formamide. This mixture remains a liquid at equilibrium with water in environments normally inhabited by humans. This invention also provides for mixtures containing formamides that include detergents. Formamide is usually regarded as a denaturant for duplex formation, destabilizing the binding that is key to detection. This invention therefore also provides for materials that form duplexes in formamide-water mixtures.

摘要翻译： DNA的特定序列通常通过包括待检测序列（“分析物”）结合以产生与Watson-Crick意义上互补的DNA分子或类似物的双链体的方法来检测。 可以含有缓冲液，盐和/或洗涤剂的水性“测定环境”中的分析物。 这种纯水系统不能无限期地暴露于环境中，然而，系统中的水将蒸发。 此外，这种系统通常支持细菌和其他生物的生长，破坏其有效性。 本发明提供了使用它们的物质和方法的组合物，其包含含有超过40％甲酰胺的测定混合物。 该混合物在通常由人类居住的环境中保持与水平衡的液体。 本发明还提供含有包含洗涤剂的甲酰胺的混合物。 甲酰胺通常被认为是双相形成的变性剂，破坏了检测关键的结合。 因此，本发明还提供了在甲酰胺 - 水混合物中形成双链体的材料。