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    Microarray hybridization assembly using a heat shrinkage bag for sealing the reaction region
    3.
    发明申请
    Microarray hybridization assembly using a heat shrinkage bag for sealing the reaction region 审中-公开
    微阵列杂交组件使用热收缩袋密封反应区域

    公开(公告)号：US20050170383A1

    公开(公告)日：2005-08-04

    申请号：US10996975

    申请日：2004-11-23

    申请人： Chien-Tsung Wu ,  Chao-Chi Pan ,  Wen-Yeh Liao ,  Chih-Wei Ho ,  Yi Chen ,  Shih-Chiao Tseng ,  Chia Wang ,  Shyh-Haur Su

    发明人： Chien-Tsung Wu ,  Chao-Chi Pan ,  Wen-Yeh Liao ,  Chih-Wei Ho ,  Yi Chen ,  Shih-Chiao Tseng ,  Chia Wang ,  Shyh-Haur Su

    IPC分类号： G01N33/53 ,  B01L3/00 ,  B01L9/00 ,  G01N33/543 ,  G01N37/00 ,  C12Q1/68 ,  C12M1/34

    CPC分类号： B01L9/527 ,  B01L3/5027 ,  B01L3/5635 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/046 ,  B01L2300/0636 ,  B01L2300/0822

    摘要： A microarray hybridization assembly for conducting hybridization assay analysis is disclosed. The microarray assembly comprises a slide, a slide cover, and a spacer disposed between the slide and slide cover. The slide cover is placed over the slide and the spacer maintains a gap between the parallel surfaces of the slide and slide cover to create a reaction region for hybridization reactions between attached probe molecules and target molecules of a hybridization solution. The slide assembly is placed into a heat shrinkage bag. The bag and slide assembly are then heated in a heat cycle sufficient to seal the bag around at least three sides of the slide assembly. An optional heat shrinkage cap can be fitted over the fourth side of the slide assembly and heated in a second heat cycle to seal the fourth side of the slide assembly after a hybridization solution is introduced into the reaction region of the slide assembly.

    摘要翻译： 公开了用于进行杂交测定分析的微阵列杂交组件。 微阵列组件包括滑动件，滑动盖和布置在滑动件和滑动盖之间的间隔件。 滑动盖被放置在载玻片上，间隔物在载玻片的平行表面和载玻片之间保持间隙，以产生用于杂交溶液的附着的探针分子和靶分子之间的杂交反应的反应区域。 将滑动组件放置在热收缩袋中。 然后在热循环中加热袋和滑动组件，足以将袋围绕滑动组件的至少三个侧面密封。 可选的热收缩帽可以安装在滑动组件的第四侧上，并且在将杂交溶液引入滑动组件的反应区域之后，在第二加热循环中加热以密封滑动组件的第四侧。