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    Method for detecting an analyte in a sample
    3.
    发明授权
    Method for detecting an analyte in a sample 有权
    检测样品中分析物的方法

    公开(公告)号：US08580577B2

    公开(公告)日：2013-11-12

    申请号：US12662896

    申请日：2010-05-10

    申请人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    发明人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    IPC分类号： G01N33/566 ,  G01N33/53 ,  G01N33/543 ,  G01N33/554 ,  G01N21/00 ,  G01N21/76 ,  C12Q1/68

    CPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N33/54306 ,  C12Q2565/101 ,  C12Q2545/114 ,  C12Q2539/101 ,  C12Q2537/125

    摘要： The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a mask obtained from imaging the sample at the emission wavelength of the second reporter and applying this mask to an image of the sample used for detecting the detection probes.

    摘要翻译： 本发明涉及一种用于检测样品中分析物的方法，包括以下步骤：提供用第一报道分子标记的检测探针，该检测探针能够结合分析物，提供固体支持物，提供结合的捕获探针或 能够结合固体支持物，其捕获探针能够结合分析物，从而将分析物浓缩在固体支持物上，使样品与检测探针，固体支持物和捕获探针接触，并检测检测探针， 其中所述检测探针的检测是在存在猝灭探针的情况下进行的，所述猝灭探针结合不结合分析物的剩余检测探针，从而至少部分地猝灭所述剩余检测探针和/或固体支持物的第一报告物的发射被标记 与第二个记者不同的第二个记者，以第二个回购的发射波长成像样品 产生从第二报道器的发射波长处成像样品获得的掩模，并将该掩模应用于用于检测检测探针的样品的图像。

    Method for detecting an analyte in a sample
    4.
    发明申请
    Method for detecting an analyte in a sample 有权
    检测样品中分析物的方法

    公开(公告)号：US20110105348A1

    公开(公告)日：2011-05-05

    申请号：US12662896

    申请日：2010-05-10

    申请人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    发明人： Silke Hinnah ,  Dagmar Lambrü ,  Sonja Dröge ,  Stefan Jäger ,  Karsten Gall ,  Werner Stürmer ,  Michaela Schäfer

    IPC分类号： C40B30/04 ,  G01N33/53

    CPC分类号： C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6837 ,  G01N33/54306 ,  C12Q2565/101 ,  C12Q2545/114 ,  C12Q2539/101 ,  C12Q2537/125

    摘要： The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a mask obtained from imaging the sample at the emission wavelength of the second reporter and applying this mask to an image of the sample used for detecting the detection probes.

    摘要翻译： 本发明涉及一种用于检测样品中分析物的方法，包括以下步骤：提供用第一报道分子标记的检测探针，该检测探针能够结合分析物，提供固体支持物，提供结合的捕获探针或 能够结合固体支持物，其捕获探针能够结合分析物，从而将分析物浓缩在固体支持物上，使样品与检测探针，固体支持物和捕获探针接触，并检测检测探针， 其中所述检测探针的检测是在存在猝灭探针的情况下进行的，所述猝灭探针结合不结合分析物的剩余检测探针，从而至少部分地猝灭所述剩余检测探针和/或固体支持物的第一报告物的发射被标记 与第二个记者不同的第二个记者，以第二个回购的发射波长成像样品 产生从第二报道器的发射波长处成像样品获得的掩模，并将该掩模应用于用于检测检测探针的样品的图像。

    Cellular assay method for identifying pkc-0 inhibitors
    5.
    发明申请
    Cellular assay method for identifying pkc-0 inhibitors 审中-公开
    用于鉴定pkc-0抑制剂的细胞测定方法

    公开(公告)号：US20090170125A1

    公开(公告)日：2009-07-02

    申请号：US11919178

    申请日：2006-04-27

    申请人： Isabelle Heit ,  Monika Baudler ,  Sascha Dammeier ,  Michaela Schäfer ,  Hubert Paul ,  Gottfried Baier ,  Stephen Shaw

    发明人： Isabelle Heit ,  Monika Baudler ,  Sascha Dammeier ,  Michaela Schäfer ,  Hubert Paul ,  Gottfried Baier ,  Stephen Shaw

    IPC分类号： C12Q1/48 ,  G01N33/573 ,  C07K16/40

    CPC分类号： G01N33/5041 ,  C07K16/40 ,  C07K16/44 ,  G01N2333/9121

    摘要： The invention relates to a method for investigating the modulating effect of a test substance on a PKCθ-dependent signal transduction pathway or for finding a PKCθ modulator in a human or animal cell, including the steps (a) contacting the cell with the test substance or with the PKCθ modulator; (b) where appropriate inducing the kinase activity of PKCθ; (c) incubating the cell under conditions which bring about phosphorylation at least of a serine or threonine residue of PKCθ; (d) where appropriate lysing the cell; and (e) determining the phosphorylation content of the at least one serine or threonine residue of PKCθ.

    摘要翻译： 本发明涉及一种用于研究测试物质对PKCθ依赖性信号转导途径或在人或动物细胞中发现PKCθ调节剂的调节作用的方法，包括步骤（a）使细胞与测试物质接触或 与PKCtheta调制器; （b）适当诱导PKCθ的激酶活性; （c）在至少引起PKCθ的丝氨酸或苏氨酸残基的磷酸化的条件下孵育细胞; （d）适当时裂解细胞; 和（e）确定PKCθ的至少一个丝氨酸或苏氨酸残基的磷酸化含量。