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    Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
    1.
    发明授权
    Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage 失效
    基于质谱或质量分离和碱基特异性切割的核酸诊断

    公开(公告)号：US06994960B1

    公开(公告)日：2006-02-07

    申请号：US09424629

    申请日：1998-05-28

    申请人： Simon Foote ,  Colleen Elso ,  Richard Simpson ,  Gavin Reid

    发明人： Simon Foote ,  Colleen Elso ,  Richard Simpson ,  Gavin Reid

    IPC分类号： C12Q1/68 ,  C12N15/00 ,  C12N15/63 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12Q1/683 ,  C12Q2565/627

    摘要： A method of detecting a mutation or a difference of one or more nucleotides between a nucleic acid molecule to be tested and a reference nucleic acid molecule, said method comprising subjecting the test nucleic acid molecule to base specific cleavage to generate oligonucleotide fragments, separating the resulting oligonucleotide fragments based on mass by MALDI-ATOF MS and/or other equivalent procedure to produce a fingerprint of then oligonucleotide fragments comprising one or more peaks wherein a peak represents the mass of each fragment and identifying an altered peak relative to a reference nucleic acid molecule subjected to the same procedure wherein the presence of an altered peak is indicative of a difference of one or more nucleotides in said tested nucleic acid molecule.

    摘要翻译： 一种检测待测核酸分子与参考核酸分子之间的一个或多个核苷酸的突变或差异的方法，所述方法包括使测试核酸分子对特异性切割进行碱基产生寡核苷酸片段，分离所得的 基于质量的基于质粒的寡核苷酸片段通过MALDI-ATOF MS和/或其他等同方法产生包含一个或多个峰的寡核苷酸片段的指纹，其中峰表示每个片段的质量，并鉴定相对于参照核酸分子的改变的峰 进行相同的程序，其中改变的峰的存在指示所述测试的核酸分子中一个或多个核苷酸的差异。