公开(公告)号：US20160010066A1

公开(公告)日：2016-01-14

申请号：US14796406

申请日：2015-07-10

Applicant: Solazyme, Inc.

Inventor： David Davis ,  George Rudenko ,  Aravind Somanchi ,  Jason Casolari ,  Scott Franklin ,  Aren Ewing

IPC: C12N9/10 ,  C12P7/64

CPC classification number: C12N9/1029 ,  C12N15/82 ,  C12P7/64 ,  C12P7/6463 ,  C12P33/00 ,  C12Y203/01041

Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.

Abstract translation: 本发明涉及KASI / KASIV型的β-酮酸酰基ACP合酶基因和由这些基因编码的蛋白质。 这些基因可以包含在核酸构建体，载体或宿主细胞中。 基因产物的表达可以改变宿主细胞的脂肪酸分布。 可以将KAS基因与FATA或FATB硫酯酶基因组合以产生产生增加量的C 8 -C 16脂肪酸的细胞。 合适的宿主细胞包括植物或微藻的质体细胞。 公开了具有新基因的油性微藻宿主细胞。

公开(公告)号：US20140178950A1

公开(公告)日：2014-06-26

申请号：US14099704

申请日：2013-12-06

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Shane Brubaker ,  George N. Rudenko ,  Riyaz Bhat ,  Matthew Shoa-Azar ,  Aravind Somanchi ,  Aren Ewing

IPC: C12P7/64

CPC classification number: C12P7/6463 ,  C12N15/52

Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.

Abstract translation: 基因工程微生物，例如原型小球藻，产生微生物油的细胞可用作食品添加剂和可再生燃料和工业化学品的来源。 本发明公开了用于增加油类生物合成的脂质体生物合成基因，其可用于增加脂质生产和改变重组微生物（包括欧洲绿叶藻或原生动物）的重组微生物中的脂肪酸和三酰甘油轮廓。