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    GENETICALLY ENGINEERED MICROBIAL STRAINS INCLUDING CHLORELLA PROTOTHECOIDES LIPID PATHWAY GENES
    4.
    发明申请
    GENETICALLY ENGINEERED MICROBIAL STRAINS INCLUDING CHLORELLA PROTOTHECOIDES LIPID PATHWAY GENES 有权
    遗传工程微生物菌株，包括氯霉素原生质体脂肪道路基因

    公开(公告)号：US20140178950A1

    公开(公告)日：2014-06-26

    申请号：US14099704

    申请日：2013-12-06

    Applicant: Solazyme, Inc.

    Inventor： Scott Franklin ,  Shane Brubaker ,  George N. Rudenko ,  Riyaz Bhat ,  Matthew Shoa-Azar ,  Aravind Somanchi ,  Aren Ewing

    IPC: C12P7/64

    CPC classification number: C12P7/6463 ,  C12N15/52

    Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.

    Abstract translation: 基因工程微生物，例如原型小球藻，产生微生物油的细胞可用作食品添加剂和可再生燃料和工业化学品的来源。 本发明公开了用于增加油类生物合成的脂质体生物合成基因，其可用于增加脂质生产和改变重组微生物（包括欧洲绿叶藻或原生动物）的重组微生物中的脂肪酸和三酰甘油轮廓。

    Tailored oils produced from recombinant oleaginous microorganisms
    5.
    发明授权
    Tailored oils produced from recombinant oleaginous microorganisms 有权
    由重组含油微生物产生的定制油

    公开(公告)号：US08633012B2

    公开(公告)日：2014-01-21

    申请号：US13650024

    申请日：2012-10-11

    Applicant: Solazyme, Inc.

    Inventor： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

    IPC: C12N1/13 ,  C12N1/12 ,  C12P7/64 ,  C12N15/56 ,  C12N9/18 ,  C07H21/04

    CPC classification number: C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

    Abstract translation: 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

    TAILORED OILS
    10.
    发明申请
    TAILORED OILS 审中-公开
    定制油

    公开(公告)号：US20150125914A1

    公开(公告)日：2015-05-07

    申请号：US14506491

    申请日：2014-10-03

    Applicant: SOLAZYME, INC.

    Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Jeffrey L. Moseley

    IPC: C12P7/64 ,  C11C1/00 ,  C11D9/00

    CPC classification number: C12P7/64 ,  C11C1/002 ,  C11D9/00 ,  C12P7/6409 ,  C12Y203/01041 ,  C12Y301/02014

    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

    Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP去饱和酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

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