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    Immortalized adult human colon epithelial cell line
    1.
    发明授权
    Immortalized adult human colon epithelial cell line 失效
    永生成人人结肠上皮细胞系

    公开(公告)号：US06194203B1

    公开(公告)日：2001-02-27

    申请号：US08839271

    申请日：1997-04-17

    申请人： Stéphanie Blum ,  Andrea Pfeifer ,  Yvonne Tromvoukis

    发明人： Stéphanie Blum ,  Andrea Pfeifer ,  Yvonne Tromvoukis

    IPC分类号： C12N508

    CPC分类号： C12N5/0679 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2503/02 ,  C12N2510/04

    摘要： Immortalized adult human colon epithelial cell line, which does not express tumour markers, which expresses metabolic markers specific for the non-immortalized human epithelial cells and metabolic differentiation markers specific for the non-immortalized epithelial cells of the human colon, and which is capable of adhering in vitro to the strain of lactic acid bacterium CNCM-1225. Serum-free culture medium characterized in that it comprises trace elements, vitamins consisting of vitamin C and retinoic acid, and hormones consisting of triiodothyronine, dexamethasone, hydrocortisone, bovine pituary gland extract, insulin, EGF and transferrin. Process for the immortalization of epithelial cells of the human colon, in which a culture of primary epithelial cells derived from the human colon is prepared, the culture is infected with a recombinant virus, the immortalized cells are cultured in the serum-free culture medium according to the invention. Process for identifying the mutagenic, toxic or beneficial effect of an agent on the metabolism of the cells of the intestinal tract, in which (1) an agent suspected of being a mutagenic, toxic or beneficial agent for the metabolism of the cells of the intestinal tract is reacted, cultured or brought into contact with a culture comprising a cell line according to the invention, and (2) the effects of the said agent on the said cell line are determined or measured. Use of the cells according to the invention as an active pharmaceutical agent. In particularly, cell line DSM ACC2258.

    摘要翻译： 永生化的成人人结肠上皮细胞系，其不表达肿瘤标志物，其表达对非永生化人上皮细胞特异性的代谢标志物和对人结肠非永生化上皮细胞特异性的代谢分化标记，其能够 体外附着于乳酸菌CNCM-1225菌株。 无血清培养基，其特征在于其包含微量元素，由维生素C和视黄酸组成的维生素，以及由三碘甲状腺原氨酸，地塞米松，氢化可的松，牛垂体腺提取物，胰岛素，EGF和转铁蛋白组成的激素。 制备人结肠上皮细胞永生化方法，其中制备了源自人结肠的原代上皮细胞的培养物，用重组病毒感染培养物，将永生化细胞在无血清培养基中培养，按照 涉及本发明。 用于鉴定药剂对肠道细胞代谢的诱变，毒性或有益作用的方法，其中（1）怀疑是诱导，有毒或有益的代谢肠细胞代谢的药剂 通道与包含根据本发明的细胞系的培养物反应，培养或接触，和（2）测定或测量所述试剂对所述细胞系的作用。 使用根据本发明的细胞作为活性药剂。 特别地，细胞系DSM ACC2258。

    Immortalized human colon epithelial cell line
    2.
    发明授权
    Immortalized human colon epithelial cell line 有权
    永生人结肠上皮细胞系

    公开(公告)号：US06395542B1

    公开(公告)日：2002-05-28

    申请号：US09593134

    申请日：2000-06-14

    申请人： Stéphanie Blum ,  Andrea Pfeifer ,  Yvonne Tromvoukis

    发明人： Stéphanie Blum ,  Andrea Pfeifer ,  Yvonne Tromvoukis

    IPC分类号： C12N508

    CPC分类号： C12N5/0679 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2503/02 ,  C12N2510/04

    摘要： Human epithelial colon immortalized cell line, which does not express tumour markers, which expresses metabolic markers specific for the non-immortalized human epithelial cells and metabolic differentiation markers specific for the non-immortalized epithelial cells of the human colon, and which is capable of adhering in vitro to the strain of lactic acid bacterium CNCM-1225. Serum-free culture medium characterized in that it comprises trace elements, vitamins consisting of vitamin C and retinoic acid, and hormones consisting of triiodothyronine, dexamethasone, hydrocortisone, bovine pituary gland extract, insulin, EGF and transferrin. Process for the immortalization of epithelial cells of the human colon, in which a culture of primary epithelial cells derived from the human colon is prepared, the culture is infected with a recombinant virus, the immortalized cells are cultured in the serum-free culture medium according to the invention. Process for identifying the mutagenic, toxic or beneficial effect of an agent on the metabolism of the cells of the intestinal tract, in which (1) an agent suspected of being a mutagenic, toxic or beneficial agent for the metabolism of the cells of the intestinal tract is reacted, cultured or brought into contact with a culture comprising a cell line according to the invention, and (2) the effects of the said agent on the said cell line are determined or measured. Use of the cells according to the invention as an active pharmaceutical agent.

    摘要翻译： 人类上皮结肠永生化细胞系，其不表达肿瘤标志物，其表达对非永生化人上皮细胞特异性的代谢标记和对人结肠非永生化上皮细胞特异的代谢分化标记，并且能够粘附 体外对乳酸菌CNCM-1225菌株进行体外试验。 无血清培养基，其特征在于其包含微量元素，由维生素C和视黄酸组成的维生素，以及由三碘甲状腺原氨酸，地塞米松，氢化可的松，牛垂体腺提取物，胰岛素，EGF和转铁蛋白组成的激素。 制备人结肠上皮细胞永生化方法，其中制备了源自人结肠的原代上皮细胞的培养物，用重组病毒感染培养物，将永生化细胞在无血清培养基中培养，按照 涉及本发明。 用于鉴定药剂对肠道细胞代谢的诱变，毒性或有益作用的方法，其中（1）怀疑是诱导，有毒或有益的代谢肠细胞代谢的药剂 通道与包含根据本发明的细胞系的培养物反应，培养或接触，和（2）测定或测量所述试剂对所述细胞系的作用。 使用根据本发明的细胞作为活性药剂。

    Serum-free culture medium for immortalized human colon epithelial cell line
    3.
    发明授权
    Serum-free culture medium for immortalized human colon epithelial cell line 有权
    用于永生化人结肠上皮细胞系的无血清培养基

    公开(公告)号：US06399381B1

    公开(公告)日：2002-06-04

    申请号：US09593135

    申请日：2000-06-14

    申请人： Stéphanie Blum ,  Andrea Pfeifer ,  Yvonne Tromvoukis

    发明人： Stéphanie Blum ,  Andrea Pfeifer ,  Yvonne Tromvoukis

    IPC分类号： C12N508

    CPC分类号： C12N5/0679 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2503/02 ,  C12N2510/04

    摘要： Human epithelial colon immortalized cell line, which does not express tumour markers, which expresses metabolic markers specific for the non-immortalized human epithelial cells and metabolic differentiation markers specific for the non-immortalized epithelial cells of the human colon, and which is capable of adhering in vitro to the strain of lactic acid bacterium CNCM-1225. Serum-free culture medium characterized in that it comprises trace elements, vitamins consisting of vitamin C and retinoic acid, and hormones consisting of triiodothyronine, dexamethasone, hydrocortisone, bovine pituary gland extract, insulin, EGF and transferrin. Process for the immortalization of epithelial cells of the human colon, in which a culture of primary epithelial cells derived from the human colon is prepared, the culture is infected with a recombinant virus, the immortalized cells are cultured in the serum-free culture medium according to the invention. Process for identifying the mutagenic, toxic or beneficial effect of an agent on the metabolism of the cells of the intestinal tract, in which (1) an agent suspected of being a mutagenic, toxic or beneficial agent for the metabolism of the cells of the intestinal tract is reacted, cultured or brought into contact with a culture comprising a cell line according to the invention, and (2) the effects of the said agent on the said cell line are determined or measured. Use of the cells according to the invention as an active pharmaceutical agent.

    摘要翻译： 人类上皮结肠永生化细胞系，其不表达肿瘤标志物，其表达对非永生化人上皮细胞特异性的代谢标记和对人结肠非永生化上皮细胞特异的代谢分化标记，并且能够粘附 体外对乳酸菌CNCM-1225菌株进行体外试验。 无血清培养基，其特征在于其包含微量元素，由维生素C和视黄酸组成的维生素，以及由三碘甲状腺原氨酸，地塞米松，氢化可的松，牛垂体腺提取物，胰岛素，EGF和转铁蛋白组成的激素。 制备人结肠上皮细胞永生化方法，其中制备了源自人结肠的原代上皮细胞的培养物，用重组病毒感染培养物，将永生化细胞在无血清培养基中培养，按照 涉及本发明。 用于鉴定药剂对肠道细胞代谢的诱变，毒性或有益作用的方法，其中（1）怀疑是诱导，有毒或有益的代谢肠细胞代谢的药剂 通道与包含根据本发明的细胞系的培养物反应，培养或接触，和（2）测定或测量所述药剂对所述细胞系的作用。 使用根据本发明的细胞作为活性药剂。