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公开(公告)号：US09951342B2
公开(公告)日：2018-04-24
申请号：US14570994
申请日：2014-12-15
CPC分类号： C12N15/746 , A23C9/123 , A23C9/1238 , A23C2220/202 , A23Y2240/75 , C12N1/20 , C12N2795/00011 , C12Q1/70 , C12Q2525/151
摘要： The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.
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公开(公告)号：US20150093473A1
公开(公告)日：2015-04-02
申请号：US14570994
申请日：2014-12-15
CPC分类号： C12N15/746 , A23C9/123 , A23C9/1238 , A23C2220/202 , A23Y2240/75 , C12N1/20 , C12N2795/00011 , C12Q1/70 , C12Q2525/151
摘要： The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.
摘要翻译： 本发明提供了与调节细胞对靶核酸或其转录产物的抗性相关的方法和组合物。 在一些优选实施方案中，本发明提供了使用一种或多种cas基因或蛋白质调节细胞对靶核酸或其转录产物的抗性的组合物和方法。 在一些实施方案中，本发明提供了用于开发和使用菌株组合和起始培养旋转的方法和组合物。 在另外的实施方案中，本发明提供用于标记和/或鉴定细菌的方法。 在一些优选的实施方案中，本发明提供了使用CRISPR基因座确定噬菌体对细胞的潜在毒力的方法，以及使用CRISPR-cas调节噬菌体的遗传序列以增加毒力水平。 在另外的实施方案中，本发明提供用于开发和使用噬菌体作为生物防治剂的方法和组合物。
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公开(公告)号：US20120301575A1
公开(公告)日：2012-11-29
申请号：US13575922
申请日：2011-01-28
CPC分类号： C12N1/20 , A23C9/1209 , A23C9/123 , A23C9/1238 , A23C2220/202 , A23Y2220/00 , A23Y2220/15 , A23Y2220/35 , A23Y2240/75 , C12N9/1205 , C12N15/01 , C12N15/746 , C12Q1/14 , C12R1/225 , C12R1/46 , C12Y207/01006
摘要： The present invention relates to a bacterial cell with texturizing property, starter cultures comprising the cell, and dairy products fermented with the starter culture.
摘要翻译： 本发明涉及具有组织化特性的细菌细胞，包含该细胞的起始培养物和用起始培养物发酵的乳制品。
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公开(公告)号：US07981651B2
公开(公告)日：2011-07-19
申请号：US12253333
申请日：2008-10-17
CPC分类号： C12N9/0044 , A23C9/1234 , A23C2220/202 , A23L33/135 , A61K35/747 , A61K38/00 , A61K2035/11 , C07K14/335 , C12N9/0008 , C12N9/1051 , C12N9/13 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/2471 , C12N9/80 , C12N9/88 , C12N9/90 , C12Q1/689 , C12Y302/01023
摘要： Lactobacillus acidophilus NCFM nucleic acid molecules, polypeptides, fragments and variants thereof are provided in the current invention. In addition, fusion proteins, antigenic peptides, and antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and cells comprising the expression vectors. Methods for producing the polypeptides of the invention and methods for their use are further provided.
摘要翻译： 本发明提供了嗜酸乳杆菌NCFM核酸分子，多肽，其片段和变体。 此外，还包括融合蛋白，抗原肽和抗体。 本发明还提供含有本发明的核酸分子和包含表达载体的细胞的重组表达载体。 还提供了制备本发明多肽的方法及其使用方法。
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5.发明授权公开(公告)号：US07498162B2
公开(公告)日：2009-03-03
申请号：US10362249
申请日：2001-09-21
CPC分类号： A23C9/1234 , A23C9/1236 , A23C2220/202 , A23L29/065 , A23L33/10 , A23Y2220/43 , A23Y2240/41 , A23Y2300/00 , A61K35/745 , A61K35/747 , A61K2039/52 , C12N1/20
摘要： The present invention pertains to novel strains of lactic acid bacteria that are capable to reduce an individual's tendency to mount allergic reactions. In particular, the present invention relates to the generation of lactic acid bacteria strains that express proteins including tolerogenic peptides and the use thereof in reducing an individual's tendency to mount allergic reactions. The invention also pertains to food or pharmaceutical compositions containing said micro-organisms or active fractions thereof.
摘要翻译： 本发明涉及能够降低个体倾向于过敏反应的新型乳酸菌菌株。 特别地，本发明涉及表达包括耐受性肽的蛋白质的乳酸菌菌株的生成及其在减少个体倾向于过敏反应的用途中的用途。 本发明还涉及含有所述微生物或其活性级分的食品或药物组合物。
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公开(公告)号：US20080248553A1
公开(公告)日：2008-10-09
申请号：US12033655
申请日：2008-02-19
申请人： Wilbert Feike Henricus Sybesma , Jeroen Hugenholtz , Igor Mierau , Maria Johanna Catharina Starrenburg , Michiel Kleerebezem , Willem Meindert De Vos
发明人： Wilbert Feike Henricus Sybesma , Jeroen Hugenholtz , Igor Mierau , Maria Johanna Catharina Starrenburg , Michiel Kleerebezem , Willem Meindert De Vos
CPC分类号： A61K38/45 , A23C2220/202 , A23L29/065 , A23L33/15 , A23V2002/00 , A61K38/50 , C12N9/1235 , C12N9/48 , C12N9/78 , C12N15/52 , C12P17/182 , A23V2300/21
摘要： The invention provides a process of producing bio-available folate, i.e., folic acid having an increased proportion of monoglutamyl folate and a decreased proportion of polyglutamyl folate, by culturing food-grade microorganisms containing an active heterologous or homologous polyglutamyl hydrolase activity or containing increased activities of folate biosynthesis enzymes. The genes encoding the polyglutamyl hydrolase and the folate biosynthesis enzymes may be of various origin, e.g. from rodents or other mammals including man. Also provided is a foodstuff, especially a dairy product containing such monoglutamyl folate-producing microorganisms.
摘要翻译： 本发明通过培养含有活性异源或同源的聚谷氨酸水解酶活性或含有增加的活性的食品级微生物提供了生产生物可用叶酸的方法，即具有增加的叶酸单谷氨酸比例增加的叶酸和降低的聚谷氨酸叶酸的比例 的叶酸生物合成酶。 编码聚谷氨酸水解酶和叶酸生物合成酶的基因可以是各种来源的，例如。 从啮齿动物或其他哺乳动物，包括人。 还提供了一种食品，特别是含有这种产生叶酸单谷氨酰胺的微生物的乳制品。
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公开(公告)号：US20070243303A1
公开(公告)日：2007-10-18
申请号：US10562601
申请日：2004-07-02
申请人： Christiaan Dan Hengst , Olivera Gajic , Oscar Kuipers , Jan Kok , Jan Sikkema , Johannes Geurts , Arjen Nauta
发明人： Christiaan Dan Hengst , Olivera Gajic , Oscar Kuipers , Jan Kok , Jan Sikkema , Johannes Geurts , Arjen Nauta
CPC分类号： C12N15/746 , A23C9/12 , A23C19/032 , A23C2220/202
摘要： The invention relates to methods and means for regulating gene expression and to CodY target sequences. CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. Also, CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus are disclosed. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.
摘要翻译： 本发明涉及调节基因表达和CodY靶序列的方法和手段。 CodY通过与相应基因上游的特定DNA序列结合来抑制其靶基因。 通过分析在DNA微阵列研究中鉴定的δ编码乳酸乳球菌MG1363衍生物中的去甲基化基因的上游序列来鉴定保守的靶位点。 此外，公开了来自其它革兰氏阳性菌如枯草芽孢杆菌和链球菌的CodY靶序列。 因此，本发明提供可用于不同应用中的抑制或解除基因表达的CodY靶序列。
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公开(公告)号：US06833260B1
公开(公告)日：2004-12-21
申请号：US09542121
申请日：2000-04-04
申请人： Frank E. Ruch
发明人： Frank E. Ruch
IPC分类号： C12N938
CPC分类号： C12R1/01 , A23C9/1206 , A23C2220/202 , C12N9/2471 , C12Y302/01023
摘要： The invention features methods and compositions for rapidly and effectively hydrolyzing lactose using recombinant lactic acid bacteria.
摘要翻译： 本发明的特征在于使用重组乳酸菌快速有效地水解乳糖的方法和组合物。
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9.发明申请公开(公告)号：US20040009490A1
公开(公告)日：2004-01-15
申请号：US10264213
申请日：2002-10-03
发明人： Matthew Glenn , Ilkka J. Havukkala , Mark Lubbers , James Dekker
IPC分类号： C12Q001/68 , C07H021/04 , C12P021/02 , C12N001/21 , C12N015/74 , C07K014/335
CPC分类号： A23C19/0323 , A23C2220/202 , A61K38/00 , A61K2039/52 , C07K14/335 , C07K2319/00
摘要： Novel polynucleotides isolated from Lactobacillus rhamnosus, as well as oligonucleotide probes and primers, genetic constructs comprising the polynucleotides, biological materials, including plants, microorganisms and multicellular organisms incorporating the polynucleotides, polypeptides expressed by the polynucleotides, and methods for using the polynucleotides and polypeptides are disclosed.
摘要翻译： 从鼠李糖杆菌分离的新型多核苷酸以及寡核苷酸探针和引物，包含多核苷酸的遗传构建体，生物材料，包括植物，包含多核苷酸的微生物和多细胞生物，由多核苷酸表达的多肽，以及使用多核苷酸和多肽的方法， 披露
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10.发明授权DNA encoding phage abortive infection protein from Lactococcus lactis and method of use thereof 失效编码乳酸乳球菌的噬菌体流产感染蛋白质的DNA及其使用方法
公开(公告)号：US5928688A
公开(公告)日：1999-07-27
申请号：US909425
申请日：1997-08-11
申请人： Sylvain Moineau , Barbara J. Holler , Peter A. Vandenbergh , Ebenezer R. Vedamuthu , Jeffrey K. Kondo
发明人： Sylvain Moineau , Barbara J. Holler , Peter A. Vandenbergh , Ebenezer R. Vedamuthu , Jeffrey K. Kondo
IPC分类号： A23C19/032 , C07K14/315 , C12N1/21 , C12N15/31 , C12N15/74 , A23C9/12 , C12N15/63
CPC分类号： C07K14/315 , A23C19/0323 , C12N15/746 , A23C2220/202 , A23Y2240/41
摘要： DNA encoding phage resistance protein which aborts infection by the phage, designated as AbiE. The DNA which is contained in a Lactococcus lactis deposited as NRRL-B-21443 and described in SEQ ID NO:1, is incorporated into a bacterium to encode the AbiE and provide phage resistance. Lactococcus and other bacteria encoding the AbiE are useful in industrial fermentations wherein phage are a problem.
摘要翻译： 编码噬菌体抗性蛋白质的DNA，其被称为AbiE的噬菌体中止感染。 包含在作为NRRL-B-21443并描述在SEQ ID NO：1中的乳酸乳球菌中包含的DNA被掺入到细菌中以编码AbiE并提供抗噬菌体。 乳球菌和编码AbiE的其他细菌可用于其中噬菌体是问题的工业发酵中。
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