利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.018 秒)

    Immobilization of active protein by cross-linking to inactive protein
    3.
    发明授权
    Immobilization of active protein by cross-linking to inactive protein 失效
    活性蛋白通过交联无活性蛋白质固定化

    公开(公告)号：US4464468A

    公开(公告)日：1984-08-07

    申请号：US369253

    申请日：1982-04-16

    申请人： Stratis Avrameas ,  Georges Broun ,  Eric Selegny ,  Daniel Thomas

    发明人： Stratis Avrameas ,  Georges Broun ,  Eric Selegny ,  Daniel Thomas

    IPC分类号： A61K38/00 ,  A61K39/44 ,  C12N11/02 ,  C07G7/00 ,  C12N11/06

    CPC分类号： A61K39/44 ,  A61K35/74 ,  A61K35/742 ,  A61K38/363 ,  A61K38/38 ,  A61K38/443 ,  A61K38/47 ,  A61K38/4826 ,  A61K38/51 ,  C12N11/02 ,  Y10S530/816

    摘要： A solution of an active protein substance and an inactive protein substance is reacted with a cross-linking agent, optionally in the presence of an inert carrier, under cross-linking conditions to produce articles comprising both active and inactive protein substances. The active protein substance comprises up to about 20 percent, e.g. from 1 to 20 percent by weight, based on the final weight of the total protein substance, whereas the cross-linking agent comprises from 0.5 to 8 percent by weight, based on the weight of the total treated mixture. The obtained articles are in the form of a solution or a suspension in aqueous medium, in the form of a film, in the form of a membrane, in the form of a fabric, in the form of a porous material, or in the form of a mass, such as granules, pills or tablets.

    摘要翻译： 将活性蛋白质物质和无活性蛋白质物质的溶液与交联剂（任选在惰性载体存在下）在交联条件下反应，以产生包含活性和非活性蛋白质物质的制品。 活性蛋白物质包含至多约20％，例如 基于总蛋白质物质的最终重量为1至20重量％，而交联剂占总处理混合物的重量的0.5至8重量％。 所获得的制品是以水溶液或悬浮液形式的水溶液或悬浮液形式的水溶液或悬浮液，形式为多孔材料形式的膜形式的薄膜形式，或形式为 的颗粒，丸剂或片剂。

    Method and composition for the detection and study of cellular activity or the like and means for applying such method
    4.
    发明授权
    Method and composition for the detection and study of cellular activity or the like and means for applying such method 失效
    用于检测和研究细胞活性等的方法和组合物以及应用这种方法的方法

    公开(公告)号：US4390620A

    公开(公告)日：1983-06-28

    申请号：US216911

    申请日：1980-12-16

    申请人： Guy-Alain Junter ,  Jean-Francois Lemeland ,  Eric Selegny ,  Jean-Claude Vingent

    发明人： Guy-Alain Junter ,  Jean-Francois Lemeland ,  Eric Selegny ,  Jean-Claude Vingent

    IPC分类号： C12Q1/00 ,  G01N27/416 ,  G01N33/487 ,  C12Q1/04 ,  C12Q1/06

    CPC分类号： C12Q1/004 ,  Y10S435/808 ,  Y10S435/817 ,  Y10S436/904

    摘要： The invention pertains to a method for the study of the behaviour or detection of cells, organites or cellular fractions, possibly present in a medium.It comprises providing said medium with the essential nutrients to promote the activity of the said cells, organites or cellular fractions, if any, as well as with an "electron transporter" such as lipoic acid which is liable of forming a redox system and of intervening in one of the metabolic routes involved by the above said activity, particularly growth of cells.The method of the invention then further comprises measuring and detecting a possible modification of the relative proportions of the oxidized and reduced form of the "electron transporter". The time required for such modification to possibly take place, as well as other parameters of the reaction can then be correlated to the presence in the medium concerned of a cellular activity or analogous.

    摘要翻译： 本发明涉及研究可能存在于培养基中的细胞，组织细胞或细胞部分的行为或检测方法。 其包括向所述培养基提供必需营养物质以促进所述细胞，组织细胞或细胞级分（如果有的话）的活性，以及​​与可能形成氧化还原体系的“电子转运蛋白”如硫辛酸和介入 在上述活性涉及的代谢途径之一，特别是细胞生长。 本发明的方法还包括测量和检测“电子转运体”的氧化还原形式的相对比例的可能修饰。 可能发生这种改变所需的时间以及反应的其它参数可以与在细胞活性或类似物相关的培养基中的存在相关。