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公开(公告)号:US5141860A
公开(公告)日:1992-08-25
申请号:US412670
申请日:1989-09-26
申请人: Stephen Bornemann , John M. Cassells , Clive L. Combes , Jonathan S. Dordik , Andrew J. Hacking
发明人: Stephen Bornemann , John M. Cassells , Clive L. Combes , Jonathan S. Dordik , Andrew J. Hacking
摘要: Sucrose derivatives partially acylated in each ring can be obtained by treating a fully or partially acylated sucrose derivative with an enzyme having sucrose esterase activity capable of removing at least one acyl group from each ring, generally comprising or being a component of a lipase, esterase, amylase, .alpha.-galactosidase or protease preparation, the enzyme treatment being effected in an aqueous system. Sucralose can be obtained by using this method to obtain a sucrose 2,3,6,3',4'-penta ester which can then be chlorinated and de-esterfied. Some of the penta acetates, tetraacetates and mixed acetates/butyrates are new compounds.
摘要翻译: 通过用能够从每个环除去至少一个酰基的蔗糖酯酶活性的酶处理完全或部分酰化的蔗糖衍生物,通常包含或作为脂肪酶,酯酶的组分,可以获得在每个环中部分酰化的蔗糖衍生物, 淀粉酶,α-半乳糖苷酶或蛋白酶制剂,酶处理在水系统中进行。 可以通过使用该方法获得三氯蔗糖,得到蔗糖2,3,6,3',4'-五酯,然后可以将其氯化和脱酯化。 一些五乙酸酯,四乙酸酯和混合乙酸酯/丁酸酯是新的化合物。
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公开(公告)号:US4617269A
公开(公告)日:1986-10-14
申请号:US622853
申请日:1984-06-21
CPC分类号: C07H5/02 , C12N9/1051 , C12P19/12 , C12P19/18 , Y10S435/839
摘要: A process for preparing a fructoside, especially a fructosyl disaccharide, comprises reacting a fructosyl saccharide such as sucrose or raffinose with an alcohol or aldose in the presence of a fructosyl-transferase, especially one derived from B. subtilis NCIB 11811, 11872 or 11873. In particular, aldose is a compound of the formula ##STR1## in which A represents a hydrogen atom or the group CH.sub.2 X, where X represents a hydrogen atom or an alkoxy group, and the fructosyl disaccharide so formed is halogenated to provide a halosucrose or halogalactosucrose sweetener.
摘要翻译: 制备果糖苷,特别是果糖基二糖的方法包括在果糖基转移酶,特别是来自枯草芽孢杆菌NCIB 11811,11872或11873的一种存在下,使果糖基糖例如蔗糖或棉子糖与醇或醛糖反应。 特别地,醛糖是式(II)的化合物,其中A表示氢原子或CH2X基团,其中X表示氢原子或烷氧基,并且如此形成的果糖基二糖被卤化以提供 卤糖或卤糖乳糖甜味剂。
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公开(公告)号:US5270460A
公开(公告)日:1993-12-14
申请号:US893407
申请日:1992-06-03
摘要: Sucrose or derivatives thereof such as esters are acylated at the 4'- and/or the 6- position by reaction with a donor acyl ester, e.g. a reactive ester of an alkanoic acid or benzoic acid, in the presence of lipase. Sucrose 6,4' so produced can be chlorinated and deacylated to produce the sweetener sucralose.
摘要翻译: 蔗糖或其衍生物如酯通过与供体酰基酯反应而在4'-和/或6-位上被酰化。 在脂肪酶存在下的链烷酸或苯甲酸的反应性酯。 如此生产的蔗糖6,4'可以被氯化和脱酰基化以产生甜味剂三氯蔗糖。
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4.
公开(公告)号:US4490467A
公开(公告)日:1984-12-25
申请号:US437604
申请日:1982-10-29
CPC分类号: C12R1/38 , C12P19/04 , Y10S435/874
摘要: A process for producing polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues comprises growing a biologically pure culture of a Pseudomonas mendocina microorganism selected from the group consisting of NCIB 11687, 11688, and 11689 in an aqueous nutrient medium by submerged aerobic fermentation of an assimilable carbon source and recovering the polysaccharide. Biologically pure cultures of the organisms are another feature of the invention.
摘要翻译: 由D-甘露糖醛酸和L-古罗糖醛酸残基的部分乙酰化的可变嵌段共聚物组成的多糖的制备方法包括将水杨酸假单胞菌(Pseudomonas mendocina)微生物的生物学纯培养物生长在NCIB 11687,11688和11689中, 营养培养基通过浸没好氧发酵可同化碳源并回收多糖。 生物体的生物纯培养物是本发明的另一特征。
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公开(公告)号:US5128248A
公开(公告)日:1992-07-07
申请号:US412904
申请日:1989-09-26
摘要: Sucrose or derivatives thereof such as esters are acylated at the 4'- and/or the 6-position by reaction with a donor acyl ester, e.g. a reactive ester of an alkanoic acid or benzoic acid, in the presence of lipase. Sucrose 6,4'-diesters so produced can be chlorinated and deacylated to produce the sweetener sucralose.
摘要翻译: 蔗糖或其衍生物如酯通过与供体酰基酯反应而在4'-和/或6-位被酰化。 在脂肪酶存在下的链烷酸或苯甲酸的反应性酯。 如此生产的蔗糖6,4'-二酯可以被氯化和脱酰基化以产生甜味剂三氯蔗糖。
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6.
公开(公告)号:US4826962A
公开(公告)日:1989-05-02
申请号:US921370
申请日:1986-10-20
申请人: Elner B. Rathbone , Khizar S. Mufti , Riaz A. Khan , Peter S. J. Cheetham , Andrew J. Hacking , Jonathan S. Dordick
发明人: Elner B. Rathbone , Khizar S. Mufti , Riaz A. Khan , Peter S. J. Cheetham , Andrew J. Hacking , Jonathan S. Dordick
IPC分类号: A23G3/38 , A23G3/34 , C07H1/00 , C07H3/04 , C07H5/02 , C12P19/00 , C12P19/14 , C12P19/20 , C12R1/645 , C12R1/685
CPC分类号: C07H5/02
摘要: The novel chlorinated sugar O-.alpha.-D-6-chloro-6-deoxygalactopyranosyl-(1.fwdarw.6)-.alpha.-D-4-chloro-4-deoxygalactopyranosyl-(1.fwdarw.2)-.beta.-D-1, 6-dichloro-1, 6-dideoxyfructofuranoside (TCR) can be used to prepare sucralose by incubating the TCR in solution in the presence of an enzyme serving to remove the 6-chloro-6-deoxygalactosyl moiety from the 6-position, especially an enzyme derived from a strain of Mortierella vinacea, Circinella muscae or Aspergillus niger. TCR is prepared by treating raffinose with thionyl chloride in the presence of triphenylphosphine oxide.
摘要翻译: 新型氯化糖O-α-D-6-氯-6-脱氧半乳糖吡喃糖基 - (1-> 6)-α-D-4-氯-4-脱氧半乳糖吡喃糖基 - (1-> 2)-β-D-1, 6-二氯-1,6-二脱氧呋喃果糖苷(TCR)可用于制备三氯蔗糖,通过在用于从6-位除去6-氯-6-脱氧半乳糖基部分的酶存在下,在溶液中温育TCR,特别是 衍生自高山被孢霉菌株,,蛇科或黑曲霉的酶。 通过在三苯基氧化膦存在下用亚硫酰氯处理棉子糖来制备TCR。
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